New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen

Judycka, Sylwia; Szczepkowski, M.; Ciereszko, Andrzej; Dietrich, G.J.

doi:10.1016/j.cryobiol.2015.02.005

Cited by 36 publications

(15 citation statements)

References 27 publications

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“…It is also observed that its suitability in semen extenders could differ according to the molecular weight, composition or structure of the sugar and the type of buffer used (An et al, 2000;Fernández-Santos et al, 2007). Moreover, the beneficial effect of sugar could be influenced in a dose-dependent manner; sugar concentration out of the range of optimal concentration may produce a dramatic decrease in post-thaw sperm quality, such as the case in rainbow trout and Siberian sturgeon (Judycka et al, 2015(Judycka et al, , 2020. It is, therefore, recommended that the type and concentration of sugar for cryopreservation need to be according to the species.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of different sugar extenders and large volume cryotube for sperm cryopreservation of brown‐marbled grouper ( Epinephelus fuscoguttatus )

Yang

Fan

Chen

et al. 2022

Aquaculture Research

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Brown‐marbled grouper (Epinephelus fuscoguttatus) is a large‐bodied coral reef fish with high ecological and economic value in South‐East Asia. Previous studies have developed cryopreservation protocols in 0.25‐ or 0.5‐ml straws, but this small volume may limit the utilization of cryopreserved sperm for large‐scale artificial fertilization, considering the high fecundity of brown‐marbled grouper. To aid its commercial application, the main objective of the present study was to further elaborate cryopreservation protocol in this species, by evaluating the cryoprotective efficacy of different sugar extenders (glucose, sucrose or trehalose) and the feasibility of a 5‐ml cryotube for cryopreservation. After frozen in a simple media containing 10% DMSO and 0.3‐M sugar extender using a 0.5‐ml straw, there was a deterioration in sperm motility, viability, mitochondrial membrane potential (MMP) and ATP content compared with fresh semen, but fertilization success of cryopreserved samples was not compromised at the ratio of 10,000 spermatozoa per egg. Besides, the type of sugar extender had no obvious effect on post‐thaw sperm quality and fertility, thus it is suggested that glucose, sucrose or trehalose are fully interchangeable for the cryopreservation of brown‐marbled grouper semen. By employing a faster freezing rate (freezing height: 2 cm vs. 3 cm) and longer thawing time (40°C, 90 s vs. 40°C, 7 s), samples cryopreserved in a 5‐ml cryotube exhibited similar post‐thaw motility parameters and fertility with those in 0.5‐ml straw. Overall, our results demonstrate the effectiveness of new sugar extenders and the applicability of a 5‐ml cryotube for sperm cryopreservation in brown‐marbled grouper, which would allow for better flexibility for large‐scale artificial fertilization in hatchery practice.

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Section: Discussionmentioning

confidence: 99%

Evaluation of different sugar extenders and large volume cryotube for sperm cryopreservation of brown‐marbled grouper ( Epinephelus fuscoguttatus )

Yang

Fan

Chen

et al. 2022

Aquaculture Research

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“…Judycka et al . () suggested the 0.1 M glucose as external CPA, that stabilizes the sperm cell membrane, in conjunction with 15% MeOH as an alternative extender for cryopreservation of Siberian sturgeon ( Acipenser baerii ) sperm.…”

Section: Discussionmentioning

confidence: 99%

“…Cabrita, Anel & Herráez ; Horvath, Wayman, Urbányi, Ware, Dean & Tiersch ; Vuthiphandchai et al . ; Boryshpolets, Dzyuba, Rodina, Alavi, Gela & Linhart ; Alavi, Hatef, Psenicka, Kaspar, Boryshpolets, Dzyuba, Cosson, Bondarenko, Rodina, Gela & Linhart ; Kutluyer, Kayım, Öğretmen, Büyükleblebici & Tuncer ; Judycka, Szczepkowski, Ciereszko & Dietrich ). The efficiency of CPAs used in sturgeon sperm cryopreservation has been found to be species‐specific (Alavi et al .…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Persian sturgeon (Acipenser persicus) sperm: effects of cryoprotectants, antioxidant, membrane stabilizer, equilibration time and dilution ratio on sperm motility and fertility

2015

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Three experiments were performed to develop protocols for cryopreservation of Persian sturgeon Acipenser persicus, sperm. In the first experiment, sperm from six males was individually split in three subsamples and cryopreserved using Modified Tsvetkova's extender (mT) supplemented with dimethyl sulfoxide (DMSO), methanol (MeOH), glycerol (Gly) and ethylene glycol (EG) at concentration of 5%, 10%, 15% and 20%. In the second set of experiments, the effects of six equilibration times (0, 5, 10, 20, 40 and 60 min) and dilution ratios (volume sperm: volume extender 1:0.5, 1:1, 1:2, 1:3, 1:5 and 1:10) and the additive advantage of bovine serum albumin (BSA; 0, 2.5, 5 and 10 mg mL À1 ) and ascorbic acid (0, 2.5, 5 and 10 U mL À1 ), on the post-thaw survival of sperm (triplicate set of six fish) were evaluated. Then, sperm was diluted in 1:1 mT extender with 10 mg mL À1 BSA with selected cryoprotectants (15% MeOH and 10% DMSO) for 5 min. After a month of storage in liquid nitrogen, post-thawed sperm motility; fertilization and hatching rate and viability of derived larvae were measured (Exp.3). Evaluation of cryoprotectants efficiency showed that MeOH 15% and DMSO 10% were suitable for cryopreservation of Persian sturgeon sperm. Gly and EG resulted in very low post-thaw motility rates even at lowest concentration. No significant difference was observed among the four different equilibration times (0, 5, 10, 20 min) (P > 0.05) although higher equilibration times than 20 min resulted low post-thaw motility (P < 0.05). The motility of frozen-thawed sperm did not significantly change when dilution ratio was increased from 1:0.5 to 1:3 (P > 0.05). However, higher dilution ratios (1:5 and 1:10) reduced the percentage of motile sperm. Supplementation of the cryoprotectant solution with 10 mg mL À1 BSA significantly improved post-thaw motility (P < 0.05), but ascorbic acid did not improve post-thaw motility (P > 0.05). The results of experiment 3 showed that the highest fertilization (30.2 AE 5.75) and hatching rates (28.2 AE 5.25) were observed when samples were frozen with 15% MeOH (P > 0.05). Our study indicates that the use of mT extender consisting of 10 mg mL À1 BSA in 15% MeOH diluted with sperm at 1:1 ratio for 5 min can be recommended cryopreservation method for Persian sturgeon sperm.

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“…1) but there is an increasing interest in designing cryopreservation protocols for species from tropical and subtropical areas (Maria et al, 2015; Viveiros and Godinho, 2009). Traditional empirical studies in a factorial experimental design testing a range of freezing rates, cryoprotectants or extenders are still conducted in order to develop cryopreservation protocols for species such as Atlantic salmon (Dziewulska et al, 2011), beluga sturgeon (Aramli et al, 2015), Siberian sturgeon (Judycka et al, 2015), perch (Bernáth et al, 2015) or tambaqui (Maria et al, 2015; Varela Junior et al, 2015). Nevertheless, the evaluation of sperm quality after freezing/thawing has evolved, allowing a better understanding of the mechanisms linking to the compromised quality during the cryopreservation process.…”

Section: Fish Sperm Cryopreservationmentioning

confidence: 99%

Cryobanking of aquatic species

et al. 2017

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This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production.

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New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen

Cited by 36 publications

References 27 publications

Evaluation of different sugar extenders and large volume cryotube for sperm cryopreservation of brown‐marbled grouper ( Epinephelus fuscoguttatus )

Evaluation of different sugar extenders and large volume cryotube for sperm cryopreservation of brown‐marbled grouper ( Epinephelus fuscoguttatus )

Cryopreservation of Persian sturgeon (Acipenser persicus) sperm: effects of cryoprotectants, antioxidant, membrane stabilizer, equilibration time and dilution ratio on sperm motility and fertility

Cryobanking of aquatic species

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