Cryobanking of aquatic species

Martínez‐Páramo, S.; Horváth, Ákos; Labbé, Catherine; Zhang, Tiantian; Robles, Vanesa; Herráez, M.P.; Suquet, Marc; Adams, Serean L.; Viveiros, Ana Tereza de Mendonça; Tiersch, Terrence R.; Cabrita, Elsa

doi:10.1016/j.aquaculture.2016.05.042

Cited by 187 publications

(170 citation statements)

References 245 publications

(314 reference statements)

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“…Within the field of ex situ conservation, germplasm cryopreservation has been repeatedly suggested as a new and important tool to for species conservation (Ballou, 1992;Jewgenow et al, 2017;Martinez-Paramo et al, 2017). Within the field of ex situ conservation, germplasm cryopreservation has been repeatedly suggested as a new and important tool to for species conservation (Ballou, 1992;Jewgenow et al, 2017;Martinez-Paramo et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…One of the ways to do so is through germplasm cryopreservation. Perhaps not surprisingly, aside from research in humans, studies on germplasm cryopreservation have largely focused on advances in technology for livestock (e.g., Morrell & Mayer, 2017;Sieme & Oldenhof, 2015;Singh, Mal, & Singla, 2017) and aquaculture management (e.g., reviews by Asturiano, Cabrita, & Horváth, 2017;Martinez-Paramo et al, 2017;Magnotti et al, 2018). Perhaps not surprisingly, aside from research in humans, studies on germplasm cryopreservation have largely focused on advances in technology for livestock (e.g., Morrell & Mayer, 2017;Sieme & Oldenhof, 2015;Singh, Mal, & Singla, 2017) and aquaculture management (e.g., reviews by Asturiano, Cabrita, & Horváth, 2017;Martinez-Paramo et al, 2017;Magnotti et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Applying cryopreservation to anuran conservation biology

Poo

Hinkson

2019

Conservat Sci and Prac

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Novel conservation methods have become increasingly important in protecting our remaining biodiversity in the face of unprecedented rates of species declines. One method of maintaining genetic and species diversity is through germplasm cryopreservation. However, our knowledge of cryopreservation relies heavily on studies in domestic or aquaculture species. Moreover, most studies are narrowly focused on a single species. Consequently, the broader application of these methods to wildlife conservation is often unknown or assumed. Here, we examine the response of four anuran species from two families (Anaxyrus fowleri, Anaxyrus baxteri, Lithobates pipiens, and Lithobates sevosus) to three sperm cryopreservation treatments (5, 10, and 15% N,N-dimethylformamide with 10% trehalose). Within each family, we selected a congeneric species pair with one common, nonthreatened species and one endangered species. We found that endangered species had significantly lower initial sperm quality, though recovery rate of sperm after cryopreservation was not significantly different between nonthreatened and endangered species. Overall, responses to the three treatments were consistent across species, with 5% DMFA with 10% trehalose producing the highest recovery rates in all four species. In addition, cryopreserved sperm were used to successfully produce hatchlings in both of the nonthreatened species. Our results demonstrate that standardized methods developed for anurans can be applied more widely across families and can be transferred from model species to species of conservation concern.These findings form a basis for further exploration into cryopreservation as an effective tool for wildlife conservation in amphibians. K E Y W O R D Samphibian, assisted reproductive technology, Bufonidae, cryobiology, Ranidae, sperm motility

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Applying cryopreservation to anuran conservation biology

Poo

Hinkson

2019

Conservat Sci and Prac

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“…Common carp ( Cyprinus carpio ) is one of the most important cultured freshwater fish in the world (FAO, ; Horváth, Tamàs, & Seagrave, ). Sperm cryopreservation was frequently studied in carp because of economical (commercial production) and recreation value (Martínez‐Páramo et al, ). However, carp sperm cryopreservation remains a problematic issue, mainly due to the low replicability of published protocols which limits the practical utilization of cryopreservation methods (Bernath et al, ; Tiersch, ).…”

Section: Introductionmentioning

confidence: 99%

The comparison of two different extenders for the improvement of large‐scale sperm cryopreservation in common carp (Cyprinus carpio)

Várkonyi

Bokor

Molnár

et al. 2018

Reprod Domestic Animals

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Contents In our study, a traditionally used (Grayling, already used in cyprinid species) and a newly tested (Pike) extender was tested to avoid sperm agglutination phenomenon following thawing during carp sperm cryopreservation. A large‐scale (elevated volume of sperm) freezing method in a controlled‐rate freezer using 5 ml straw and 10 ml cryotube was also systematically established. In all experiments, the sperm cryopreserved in using Grayling extender (except only one sample) showed an agglutination phenomenon (damaged and intact cells adhered to each other) after thawing where Pike extender resulted the regular cell suspension. No significant difference was observed between the two cryopreserved groups (Pike and Grayling extender) in all motility parameters using the 0.5 ml straw and the polystyrene box. Similarly, motility parameters did not show a significant difference in the two frozen groups with the 5 ml straw, also in the polystyrene box. A significantly higher progressive motility (pMOT, Grayling: 54% ± 8%, Pike: 37% ± 5%), straight line velocity (VSL, Grayling: 50 ± 5 µm/s, Pike: 39 ± 4 µm/s) and beat cross frequency (BCF, Grayling: 20 ± 1 Hz, Pike: 17 ± 1 Hz) was observed in the case of the grayling extender by the 5 ml straw cryopreserved in a controlled‐rate freezer (CRF) compare to the pike extender. A significantly higher VSL (Grayling: 45 ± 3 µm/s, Pike: 38 ± 4 µm/s) was observed by the grayling extender using the 10 ml cryotube than with the pike extender. Despite the randomly occurring differences in a few parameters, our new controlled freezing method using the newly tested Pike extender, the 5 ml straw or the 10 ml cryotube can be a good solution for the preservation of elevated volume of carp sperm.

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“…; Martínez‐Páramo et al . ). In addition, DNA damage is related to an increase in mutagenic alterations in genes associated with embryonic development (Valcarce et al .…”

Section: Effects Of Cryopreservation On Dna Quality and Gene Expressionmentioning

confidence: 97%

Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development

Figueroa

Lee‐Estévez

Valdebenito

et al. 2018

Reviews in Aquaculture

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Sperm DNA quality and gene expression are crucial for early embryo development. Abnormal genomic processes can cause irreversible damage to totipotent cells, thereby altering the capacity for cell differentiation. Cryopreservation is a complex procedure that exposes cells to extreme conditions; it is therefore important to determine whether cryopreservation affects genomic stability. Despite this, few studies have focused on the effects of cryopreservation on the genomic stability of fish sperm. Thus, information is lacking to determine how the cryopreservation of fish sperm affects embryo quality and subsequently aquaculture. The aim of this review was to compile background information pertaining to the study of genomic stability in cryopreserved sperm. In this way, we hope to characterize more clearly the embryonic development of fish that are of biological and economic interest. We also discuss the potential use of antioxidants in cryopreservation to help preserve DNA integrity, gene expression and embryo quality. There is a current need to evaluate the use of potential biomarkers of genomic integrity to establish the effects of cryopreservation on the DNA quality of fish gametes and embryos.

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Cryobanking of aquatic species

Cited by 187 publications

References 245 publications

Applying cryopreservation to anuran conservation biology

Applying cryopreservation to anuran conservation biology

The comparison of two different extenders for the improvement of large‐scale sperm cryopreservation in common carp (Cyprinus carpio)

Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development

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