Fertilization of bovine oocytes induced solely with combined laser microbeam and optical tweezers

Clement-Sengewald, A.; Schütze, Karin; Ashkin, A.; Palma, G.A.; Kerlen, Gertrude; Brem, Gottfried

doi:10.1007/bf02065947

Cited by 57 publications

(30 citation statements)

References 10 publications

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“…UV drilling of channels in the zona pellucida of oocytes was shown to assist sperm penetration (101). Tweezers was used to insert selected sperm into channels to effect fertilization (102,103). However, fertilization efficiency and questions of possible genetic damage must be further studied.…”

Section: ⁄2␣ٌementioning

confidence: 99%

Optical trapping and manipulation of neutral particles using lasers

Ashkin¹

1997

Proc. Natl. Acad. Sci. U.S.A.

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1,321

774

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The techniques of optical trapping and manipulation of neutral particles by lasers provide unique means to control the dynamics of small particles. These new experimental methods have played a revolutionary role in areas of the physical and biological sciences. This paper reviews the early developments in the field leading to the demonstration of cooling and trapping of neutral atoms in atomic physics and to the first use of optical tweezers traps in biology. Some further major achievements of these rapidly developing methods also are considered.

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Section: ⁄2␣ٌementioning

confidence: 99%

Optical trapping and manipulation of neutral particles using lasers

Ashkin¹

1997

Proc. Natl. Acad. Sci. U.S.A.

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1,321

774

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“…For example, monozygotic twinning [19] and early embryonic demise [20] have been attributed to overly narrow artificial gaps in the zona pellucida, whereas over-large gaps could not only cause polyspermy but also could easily permit embryo loss during intra-uterine replacement. Lasers have been proposed as the tool most likely to produce precisely sized gaps, and there have been reports of their successful use for PZD [21][22][23]. However, its efficacy and safety remain to be solved in more detail.…”

Section: Partial Zona Dissection (Pzd)mentioning

confidence: 99%

Current Status and Future of Micromanipulation-Assisted Fertilization in Animals and Human.

Goto

1997

Journal of Reproduction and Development

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“…Lasers have been used not only for assisted hatching, but also for sperm immobilization and selection, and for embryo biopsy for preimplantation genetic diagnosis as well. In earlier papers it has been shown that nanosecond lasers may be successfully applied for embryo microsurgery [12,13]. At the present time picosecond [14] and femtosecond (fs) lasers [15][16][17] are mainly used.…”

Section: Introductionmentioning

confidence: 99%

Noncontact laser microsurgery of three-dimensional living objects for use in reproductive and regenerative medicine

Ситников

Ilina

Kosheleva

et al. 2018

J. Phys.: Conf. Ser.

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Abstract. Laser microsurgery has enabled us to make highly precise and delicate processing of living biological specimens. We present the results of using femtosecond (fs) laser pulses in assisted reproductive technologies. Femtosecond laser dissection of outer shells of embryos (so-called laser-assisted hatching) as well as laser-mediated detachment of the desired amount of trophectoderm cells (so-called embryo biopsy) required for preimplantaion genetic diagnosis were successfully performed. The parameters of laser radiation were optimized so as to efficiently perform embryo biopsy and preserve the viability of the treated embryos. Effects of application of fs-laser radiation in the infrared (1028 nm) and visible (514 nm) wavelength ranges were studied. We also applied laser microsurgery to develop a new simple reproducible model for studying repair and regeneration in vitro. Nanosecond laser pulses were applied to perform localized microdissection of cell spheroids. After microdissection, the edges of the wound surface opened, the destruction of the initial spheroid structure was observed in the wound area, with surviving cells changing their shape into a round one. It was shown that the spheroid form partially restored in the first six hours with subsequent complete restoration within seven days due to remodeling of surviving cells.

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Fertilization of bovine oocytes induced solely with combined laser microbeam and optical tweezers

Abstract: The results indicate that a combined UV-laser microbeam and optical tweezers trap can be used successfully for "noncontact" microinsemination procedures.

Cited by 57 publications

References 10 publications

Optical trapping and manipulation of neutral particles using lasers

Optical trapping and manipulation of neutral particles using lasers

Current Status and Future of Micromanipulation-Assisted Fertilization in Animals and Human.

Noncontact laser microsurgery of three-dimensional living objects for use in reproductive and regenerative medicine

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