Current Status and Future of Micromanipulation-Assisted Fertilization in Animals and Human.

Goto, Kazufumi

doi:10.1262/jrd.43.107

Cited by 5 publications

(4 citation statements)

References 141 publications

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“…In the present study, we compared the abilities of dead, immotile and motile sperm to decondense and to form MPN. No significant difference was found, which supports the suggestion by Goto (1997) that sperm viability is not necessary for ICSI as long as the sperm's DNA and centriole are intact. Immobilization of sperm by mechanical treatment before ICSI is considered to be important for obtaining a higher fertilization rate in humans (Payne 1995), although some researchers reported opposite results (Lacham-Kaplan and Trounson 1994;Liu et al 1994).…”

Section: Discussionsupporting

confidence: 86%

“…Because normal fertilization and pregnancy rates similar to those with IVF can be achieved with ICSI in humans, the technique has been widely applied to overcome severe male infertility. ICSI could also have potential value in the conservation of endangered species, and useful applications in farm animals, such as using cytometrically sorted X-and Y-bearing spermatozoa, which are limited in number and low in motility, to produce sex-predetermined embryos through ICSI (Gordon 1994;Goto 1997).…”

Section: Introductionmentioning

confidence: 99%

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Effects of bull, sperm type and sperm pretreatment on male pronuclear formation after intracytoplasmic sperm injection in cattle

Wei

Fukui²

1999

Reprod. Fertil. Dev.

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This study investigated the effects of the bull, sperm type (dead, immotile or motile) and sperm pretreatment (i.e. mechanical (tail-cutting or tail-scoring) or chemical (heparin, heparin + caffeine, calcium ionophore A23187 or dithiothreitol)) on male pronuclear formation after intracytoplasmic sperm injection (ICSI) in cattle. Three experiments were conducted. In Experiment 1, spermatozoa from three bulls (A, B and C) were used for both ICSI and in vitro fertilization (IVF). The results were that sperm from bull B yielded a higher penetration/male pronuclear formation rate than that of bull C when used for IVF (89.6% v 25.6%, P<0.01). However, when injected into oocytes by ICSI, sperm from bull C had a higher male pronuclear formation rate than that of bull B (34.6% v. 16.1%, P<0.05). The effects of sperm type and mechanical pretreatment were examined in Experiment 2. No significant difference was found in the male pronuclear formation rate when the three types of sperm were injected into oocytes. Tail-scored sperm achieved a higher male pronuclear rate than that of non-mechanically treated ones (38.2% v. 13.2%, P<0.005). In Experiment 3, chemical pretreatments were tested and compared. Higher male pronuclear rates, compared with the control, were obtained when sperm were pretreated with heparin + caffeine, calcium ionophore A23187 and dithiothreitol (48.2%, 62.5% and 64.5% v. 25.0%, P<0.05, 0.005 and 0.005, respectively). These results indicate that (1) there is a bull variation in male pronuclear formation with ICSI, and the male pronuclear rate by ICSI is not coincident with the results by IVF, (2) immobilization of a spermatozoon by tail-scoring before ICSI can improve the formation of the male pronucleus, and (3) an appropriate chemical pretreatment of spermatozoa is necessary to achieve a higher rate of male pronuclear formation.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Effects of bull, sperm type and sperm pretreatment on male pronuclear formation after intracytoplasmic sperm injection in cattle

Wei

Fukui²

1999

Reprod. Fertil. Dev.

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“…In addition, ICSI has become an important method to study the mechanisms of fertilization and early events of egg activation in mammals (Yanagimachi 2005). ICSI involves the fertilization of oocytes in metaphase II stage (MII) by direct injection of a spermatozoon (Goto 1997). This procedure is nearly as effective as in vitro fertilization (IVF) in producing offspring in humans (Palermo et al 1992) and mice (Kimura & Yanagimachi 1995), and it has been a valuable tool for conservation purposes in species where other assisted reproductive technologies are not available or are not optimized (Perry et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Defective sperm head decondensation undermines the success of ICSI in the bovine

et al. 2017

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The efficiency of intracytoplasmic sperm injection (ICSI) in the bovine is low compared to other species. It is unknown whether defective oocyte activation and/or sperm head decondensation limit the success of this technique in this species. To elucidate where the main obstacle lies, we used homologous and heterologous ICSI and parthenogenetic activation procedures. We also evaluated whether maturation negatively impacted the early stages of activation after ICSI. Here we showed that injected bovine sperm are resistant to nuclear decondensation by bovine oocytes and this is only partly overcome by exogenous activation. Remarkably, when we used heterologous ICSI,-matured mouse eggs were capable of mounting calcium oscillations and displaying normal PN formation following injection of bovine sperm, although -matured mouse oocytes were unable to do so. Together, our data demonstrate that bovine sperm are especially resistant to nuclear decondensation by-matured oocytes and this deficiency cannot be simply overcome by exogenous activation protocols, even by inducing physiological calcium oscillations. Therefore, the inability of a suboptimal ooplasmic environment to induce sperm head decondensation limits the success of ICSI in the bovine. Studies aimed to improve the cytoplasmic milieu of -matured oocytes and to replicate the molecular changes associated with capacitation and acrosome reaction will deepen our understanding of the mechanism of fertilization and improve the success of ICSI in this species.

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“…Intracytoplasmic sperm injection (ICSI) has become a leading tool used to study sperm function in fertilization and embryonic development in humans and animals (Yanagimachi, ). ICSI involves the fertilization of an egg in metaphase II by direct injection of a spermatozoon with intact plasma and acrosomal membranes (Goto, ). This procedure is nearly as effective as in vitro fertilization (IVF) at producing offspring in humans (Palermo, Joris, Devroey, & Van Steirteghem, ; Van Steirteghem et al, ) and mice (Kimura & Yanagimachi, ; Lacham‐Kaplan & Trounson, ).…”

Section: Introductionmentioning

confidence: 99%

Sperm capacitation pretreatment positively impacts bovine intracytoplasmic sperm injection

Águila

Zambrano

Arias

et al. 2017

Molecular Reproduction Devel

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The efficiency of intracytoplasmic sperm injection (ICSI) in bovines is low compared to other species due in part to inadequate egg activation and sperm nucleus decondensation after injection. We hypothesized that this low efficiency is due to the lack of complete sperm capacitation, so we evaluated the effects of isobutylmethylxanthine (IBMX) and methyl-β-cyclodextrin (MβCD) on bovine sperm capacitation and on the preimplantation developmental potential of bovine embryos generated by ICSI. Treatment with IBMX and MβCD decreased sperm viability (between 13-30%); nevertheless, 0.4 mM IBMX and 1 mM MβCD increased (p < 0.05) capacitation metrics-that is, acrosome exocytosis, intracellular calcium level, plasma membrane fluidity, and tyrosine phosphorylation-compared to the control. After ICSI, embryos injected with IBMX- and MβCD-treated sperm showed similar cleavage to the untreated group (range 82-88%). Pronucleus formation rate was higher with MβCD-pretreatment (54%) compared to the control group (25%), and blastocyst rate was significantly improved with MβCD-pretreatment (24%) compared to the IBMX (18%) and control (17%) groups. Importantly, embryo quality-as assessed by the total number of cells, cell allocation, and apoptotic cell index-was not affected by the sperm treatments. In conclusion, MβCD pretreatment of sperm improved the efficiency of blastocyst production in bovine ICSI.

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Current Status and Future of Micromanipulation-Assisted Fertilization in Animals and Human.

Cited by 5 publications

References 141 publications

Effects of bull, sperm type and sperm pretreatment on male pronuclear formation after intracytoplasmic sperm injection in cattle

Effects of bull, sperm type and sperm pretreatment on male pronuclear formation after intracytoplasmic sperm injection in cattle

Defective sperm head decondensation undermines the success of ICSI in the bovine

Sperm capacitation pretreatment positively impacts bovine intracytoplasmic sperm injection

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