Optical trapping and manipulation of neutral particles using lasers

Ashkin, A.

doi:10.1073/pnas.94.10.4853

Cited by 1,303 publications

(776 citation statements)

References 153 publications

Supporting

Mentioning

768

Contrasting

Unclassified

Order By: Relevance

“…For example, the speed was measured at 7-8 mm s 21 at room temperature for a channel whose geometry was of 400 mm width, 80 mm height and 10 mm length, indicating that the evaporation rate was 0.224-0.256 nL s 21 . In this case, it might take 20-30 min to evaporate the entire medium in the channel based on the channel dimension.…”

Section: Surface Tension-driven Capillary Flow and Subsequent Generatmentioning

confidence: 99%

“…Recently, laminar flow patterning, 15 pre-patterning with adhesive ligands, 16,17 and immobilization inside hydrogels 18 have been used to immobilize anchorage-dependent cells within microchannels. An alternative approach to patterning cells is based on cell-capturing or cell-trapping including hydrodynamic confinement, 19 negative dielectrophoresis, 20 optical tweezers, 21 and microwells etched at the tip of a fiber-optic bundle. 22 These methods, however, would have some limitations for easy, cheap, high-throughput microscopic studies of single cells.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pumpless, selective docking of yeast cells inside a microfluidic channel induced by receding meniscus

et al. 2006

View full text Add to dashboard Cite

We present a simple cell docking method induced by receding meniscus to capture non-adherent yeast cells onto microwells inside a microfluidic channel. Microwells were fabricated either by capillary moulding of UV curable polyurethane acrylate (PUA) onto glass substrate or direct replica moulding of poly(dimethyl siloxane) (PDMS). A cell suspension of the budding yeast, Saccharomyces cerevisiae, was introduced into the microfluidic channel by surface tension driven capillary flow and a receding meniscus was subsequently generated by evaporation. As the meniscus progressed, one to multiple yeast cells were spontaneously captured onto microwells by lateral capillary force created at the bottom of the meniscus. Using this cell-based platform, we observed the response of yeast cells upon stimulation by a mating pheromone (a-factor) by monitoring the expression of green fluorescent protein (GFP) with time. It was observed that a-factor triggered the expression of GFP at 60 min after stimulation and the fluorescence intensity was sustained for an additional 60 min without changes.

show abstract

Section: Surface Tension-driven Capillary Flow and Subsequent Generatmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pumpless, selective docking of yeast cells inside a microfluidic channel induced by receding meniscus

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Dielectric particles, such as polystyrene beads or bacteria, are attracted to the center of a tightly focused laser beam and can be trapped there. 47,48 The force exerted on the object depends on the power of the laser, the dimensions of the object, and the difference in index of refraction between the object and the surrounding medium. The large range in forces that can be applied (100 fN-100 pN) makes optical trapping techniques suitable for the investigation of the effect of force on biochemical processes.…”

Section: Mechanical Manipulation Of Individual Dna Moleculesmentioning

confidence: 99%

Honey, I shrunk the DNA: DNA length as a probe for nucleic‐acid enzyme activity

Oijen

2006

Biopolymers

View full text Add to dashboard Cite

The replication, recombination, and repair of DNA are processes essential for the maintenance of genomic information and require the activity of numerous enzymes that catalyze the polymerization or digestion of DNA. This review will discuss how differences in elastic properties between single‐ and double‐stranded DNA can be used as a probe to study the dynamics of these enzymes at the single‐molecule level. © 2006 Wiley Periodicals, Inc. Biopolymers 85: 144–153, 2007.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

show abstract

“…A bulk effect is required in the absence of tangential forcing. Radiation pressure is nonetheless known to push suspended particles, as demonstrated by optical levitation [74], because light momentum changes direction when it is elastically scattered by the particles. This momentum change results in a scattering force applied to the particle.…”

Section: Light-induced Bulk Flowmentioning

confidence: 99%

Laser microfluidics: fluid actuation by light

Delville¹,

Vincent²,

Schroll³

et al. 2009

J. Opt. A: Pure Appl. Opt.

View full text Add to dashboard Cite

The development of microfluidic devices is still hindered by the lack of robust fundamental building blocks that constitute any fluidic system. An attractive approach is optical actuation because light field interaction is contactless and dynamically reconfigurable, and solutions have been anticipated through the use of optical forces to manipulate microparticles in flows. Following the concept of "optical chip" advanced from the optical actuation of suspensions, we propose in this survey new routes to extend this concept to microfluidic two-phase flows. First, we investigate the destabilization of fluid interfaces by the optical radiation pressure and the formation of liquid jets. We analyze the droplet shedding from the jet tip and the continuous transport in laser-sustained liquid channels. In a second part, we investigate a dissipative light-flow interaction mechanism consisting in heating locally two immiscible fluids to produce thermocapillary stresses along their interface. This opto-capillary coupling is implemented in adequate microchannel geometries to manipulate two-phase flows and propose a contactless optical toolbox including valves, droplet sorters and switches, droplet dividers or droplet mergers. Finally, we discuss radiation pressure and opto-capillary effects in the context of the "optical-chip" where flows, channels and operating functions would all be performed optically on the same device.

show abstract

Optical trapping and manipulation of neutral particles using lasers

Cited by 1,303 publications

References 153 publications

Pumpless, selective docking of yeast cells inside a microfluidic channel induced by receding meniscus

Pumpless, selective docking of yeast cells inside a microfluidic channel induced by receding meniscus

Honey, I shrunk the DNA: DNA length as a probe for nucleic‐acid enzyme activity

Laser microfluidics: fluid actuation by light

Contact Info

Product

Resources

About