Fertile transgenic barley by particle bombardment of immature embryos

Ritala, Anneli; Aspegren, Kristian; Kurten, Ulrika; Salmenkallio-Marttila, Marjatta; Mannonen, Leena; Hannus, Riitta; Kauppinen, Veli; Teeri, Teemu H.; Enari, Tor-Magnus

doi:10.1007/bf00020170

Cited by 122 publications

(38 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, alternative transformation methods such as gene delivery by electrophoresis (Ahokas 1989), biolistic particle bombardment, electroporation and micro-injection were developed. Among the first reports on stable transformation of barley were those published by teams of researchers at the Institute of Biotechnology, UH, and the VTT Technological Research Centre Finland (VTT) who used particle bombardment as the method for transformation (Ritala et al 1993(Ritala et al , 1994. Later, successful methods for genetic transformation of barley were also reported with A. tumefaciens (Tingay et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Elomaa¹,

Joensuu²,

Korpelainen³

et al. 2008

AFSci

View full text Add to dashboard Cite

Plants bind solar energy to organic matter via photosynthesis and assimilation of carbon dioxide from the atmosphere and comprise the major source of nutrition and bioenergy. Plant biotechnology contributes to solution of important constraints in food and feed production and creates new technologies and applications for the sustainable use of plant resources. Genome-wide approaches such as massive parallel sequencing and microarrays to study gene expression, molecular markers for selection of important traits in breeding, characterization of genetic diversity with the aforementioned approaches, and somatic hybridization and genetic transformation are important tools in plant biotechnology. In this paper, studies carried out on enhanced resistance to viruses and tolerance of cold stress in potato, genetic modification of flower pigmentation and morphology in gerbera, production of edible vaccines in transgenic barley seeds, and expression of heterologous proteins for pharmaceutical purposes from vector viruses were chosen to exemplify the general utility of biotechnological approaches and also how plant biotechnology research has developed on cultivated plants at University of Helsinki. The studies reveal cellular and genetic mechanisms and provide scientific information that can be used for widening the uses of crop plants. They can also be used to detect any putative risks associated with the use of the biotechnological application in agriculture and horticulture and to develop practises which reduce any inadvertent negative consequences that plant production may have to the environment.

show abstract

Section: Introductionmentioning

confidence: 99%

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Elomaa¹,

Joensuu²,

Korpelainen³

et al. 2008

AFSci

View full text Add to dashboard Cite

show abstract

“…In fact, from a practical point of view, agronomically superior cultivars adapted to the region should only be used despite their recalcitrance to tissue culture methods in order to achieve gains in terms of production and quality. A few successful reports are available for commercially important barley varieties including the Finnish elite cultivar Kymppi (Ritala et al 1994 ;Nuutila et al 1999 ); several German spring cultivars (Koprek et al 1996 ;Sharma et al 2006 ); the North American cultivars, Harrington, Galena and Conlon (Cho et al 1998 ;Zhang et al 1999 ;Manoharan and Dahleen 2002 ;Manoharan et al 2006 ;Tobias et al 2007 ); the elite Australian barley cultivars, Schooner, Chebec and Sloop Murray et al 2004 ); and a Bulgarian winter barley (Kamenarova et al 2007 ). However, in all these studies transformation effi ciency was reported to be low.…”

Section: Genotype Dependencymentioning

confidence: 92%

“…The feasibility of microprojectile bombardment to transfer and express foreign DNA in barley cells was demonstrated by Mendel et al ( 1989 ) and Kartha et al ( 1989 ). Consequently, the generation of fertile, transgenic barley was achieved using different targets like immature embryos (Wan and Lemaux 1994 ;Ritala et al 1994 ;Hagio et al 1995 ;Koprek et al 1996 ;Jensen et al 1996 ), microspores (Jähne et al 1994 ;Leckband and Lörz 1998 ;Shim et al 2009 ), embryogenic callus from immature embryos (Cho et al 1998 ;Manoharan and Dahleen 2002 ) and mature embryos (Um et al 2007 ) as well as in vitro shoot meristematic cultures derived from germinated seedlings (Zhang et al 1999 ).…”

Section: Development Of Dna Delivery Techniquesmentioning

confidence: 98%

Barley

Schulze¹

2013

Alien Gene Transfer in Crop Plants, Volume 2

View full text Add to dashboard Cite

Barley ( Hordeum vulgare L.) is a widely adapted cereal crop with an extremely wide geographic distribution throughout the world. It fi nds great use for animals as a feed and for humans as a grain, especially as the source for malt for the brewing industry. In recent times, there is considerable interest in the nutritional properties of barley due to the discovery of the cholesterol-lowering effect of β-glucan, a cell wall polysaccharide. Exploitation of genetic diversity in the primary and secondary gene pool of barley using DNA-based technologies has yielded interspecifi c crosses with improved grain properties, malting quality and resistance to biotic and abiotic stresses. The signifi cant achievements regarding introgression of alien genes include the genes Rym14(Hb) , Rym16(Hb) and Ryd4(Hb) which were introgressed from Hordeum bulbosum conferring resistance to BaMMV, BaYMV and BYDV in barley. Signifi cant advances in genetic engineering of barley have been obtained, and strategies for establishment of regenerative cell and tissue culture systems as well as for development of DNA delivery techniques have been formulated. Lately, a huge potential has been realised in barley grains to produce pharmaceutical proteins like oral vaccines, growth supplements and food additives which are being exploited in a commercial scale. Nevertheless, several problems still remain like the strong genotype dependency of barley transformation protocols, transformation effi ciency, transgene stability and public acceptance. The review focuses on all these issues and elaborates achievements made in the last two decades in genetic enhancement of barley using different alien gene transfer approaches.

show abstract

“…Transformasi genetik melalui penembakan partikel dengan gen pelapor (reporter gene) telah dilaporkan pada tanaman pepaya (Fitch et al 1990), padi (Christou et al 1991), rumput (Zhong et al 1991), sorgum (Casas et al 1993), barley (Ritala et al 1994) dan bit gula (Bower et al 1996).…”

Section: Pendahuluanunclassified

Transformasi Genetik Kedelai dengan Gen Proteinase Inhibitor II Menggunakan Teknik Penembakan Partikel

et al. 2016

View full text Add to dashboard Cite

Genetic Transformation of Soybean Using the ProteinaseInhibitor II Gene by the Particle Bombardment Technique. Saptowo J. Pardal, G.A. Wattimena, H. Aswidinnoor, and M. Herman. An experiment was conducted at the Molecular Biology and Genetic Engineering Laboratory of BB-Biogen, Bogor with an objective to obtain transgenic soybean plants containing the proteinase inhibitor II (pinII) gene. The experiment consisted of three steps, i.e., optimalization of the soybean transformation technique using the gus gene; transformation of soybean using the pinII gene, and molecular analysis of the transformed soybean plants. Two type of explants (young embryo and cotyledon) were bombarded with pRQ6 plasmid containing the gus gene with the following treatment: Helium gas pressure (1100 psi and 1300 psi), shoot distance (5 and 7 cm), and number of bombardment (1x and 2x). The result of gus assay indicated that the best bombardment was done on young cotyledon explants with 1100 psi Helium pressure, shoot distance 5 cm, and 1x bombardment. Transformation of the soybean explant using the pinII gene (inside the pTWa plasmid) was conducted using the best bombardment treatment from the first activity. Two plants from c.v. Wilis (WP 1 , WP 2 ) and three plants from c.v. Tidar (TP 1 , TP 2 , TP 3 ) were recovered from regeneration and selection of the transformed explants. Molecular analysis of the regenerated plants using the PCR technique showed that only WP 2 contained the pinII gene. This plant was fertile and will be used for further evaluation.

show abstract

Fertile transgenic barley by particle bombardment of immature embryos

Cited by 122 publications

References 24 publications

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Barley

Transformasi Genetik Kedelai dengan Gen Proteinase Inhibitor II Menggunakan Teknik Penembakan Partikel

Contact Info

Product

Resources

About