Transformasi Genetik Kedelai dengan Gen Proteinase Inhibitor II Menggunakan Teknik Penembakan Partikel

Abstract

Genetic Transformation of Soybean Using the ProteinaseInhibitor II Gene by the Particle Bombardment Technique. Saptowo J. Pardal, G.A. Wattimena, H. Aswidinnoor, and M. Herman. An experiment was conducted at the Molecular Biology and Genetic Engineering Laboratory of BB-Biogen, Bogor with an objective to obtain transgenic soybean plants containing the proteinase inhibitor II (pinII) gene. The experiment consisted of three steps, i.e., optimalization of the soybean transformation technique using the gus gene; transformation of soybean using the pinII gene, and molecular analysis of the transformed soybean plants. Two type of explants (young embryo and cotyledon) were bombarded with pRQ6 plasmid containing the gus gene with the following treatment: Helium gas pressure (1100 psi and 1300 psi), shoot distance (5 and 7 cm), and number of bombardment (1x and 2x). The result of gus assay indicated that the best bombardment was done on young cotyledon explants with 1100 psi Helium pressure, shoot distance 5 cm, and 1x bombardment. Transformation of the soybean explant using the pinII gene (inside the pTWa plasmid) was conducted using the best bombardment treatment from the first activity. Two plants from c.v. Wilis (WP 1 , WP 2 ) and three plants from c.v. Tidar (TP 1 , TP 2 , TP 3 ) were recovered from regeneration and selection of the transformed explants. Molecular analysis of the regenerated plants using the PCR technique showed that only WP 2 contained the pinII gene. This plant was fertile and will be used for further evaluation.

Analisis Molekuler Gen Partenokarpi DefH9-RI-iaaM pada Progeni Tomat Transgenik

“…Berdasarkan hasil penelitian Dai et al (2001), integrasi gen insert relatif lebih stabil pada tanaman padi transgenik hasil transformasi menggunakan Agrobacterium daripada melalui penembakan partikel (particle bombardment). Hasil yang sama juga ditunjukkan pada penelitian Pardal et al (2005), yaitu transformasi tanaman kedelai dengan gen pinII menggunakan Agrobacterium lebih stabil dibanding dengan penembakan partikel.…”