Fermentative Production of Pyranone Derivate I from Marine Vibrio sp. SKMARSP9: Isolation, Characterization and Bioactivity Evaluation

Abstract

Pyranone derivative I was isolated from fermented broth of isolated marine bacterial strain Vibrio sp. SKMARSP9. The compound I was characterized, and evaluated for its antimicrobial properties. The isolated strain was identified based on 16S rRNA based phylogenetic analysis. The molecular analysis data suggested that this strain is closely related to Vibrio ruber, Vibrio sp. MSSRF10 and Vibrio rhizosphaerae. The best fermentative growth of this isolate was achieved under halophilic conditions and grew efficiently at 30°C in the presence of 12 % NaCl. The compound I production by this strain is associated with growth. The unpurified extract is hydrophobic in nature, and released only during late growth phase. The extract was purified and characterized by spectral data using NMR, DEPT, and ESI-MS. The purity of I was 97 % which was confirmed by HPLC. The pyranone derivative I exhibited [50 % antioxidant activity and broad spectrum antimicrobial properties against gram negative and gram positive strains. Molecular docking analysis revealed that this pyranone derivative I may be a potential candidate at pharmaceutical sector.

Studies on wound healing potential of red pigment isolated from marine Bacterium Vibrio sp.

“…Of the three compounds the second pure compound was obtained as a red colour liquid and was found to exhibit significant biological actions. These spectroscopic analyses led to the assignment of structure for the new compound as pyranone benzoate derivative i.e., Ethyl 4-(6-oxo tetra hydro-2H-pyron-2carbonyl) benzoate where reported in our earlier (Krishna et al, 2015).…”

“…The conversion of the raw data to this information is called assigning the spectrum. The 'H-NMR spectra of the purified Red pigment was analyzed at Indian institute of chemical technology (IICT), HYDERABAD Andhra Pradesh, INDIA (Krishna et al, 2015).…”

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“…Seawater and soil samples were collected from coastal locations of Nellore, Visakha Patnam and Bapatla, under aseptic conditions and were processed within 1-2 hours after collection [11,12]. The isolated bacteria were screened and identified by molecular characterization (using 16s rRNA sequencing), as per our previous study [13].…”

Wound healing Activities of the bioactive compounds from Micrococcus sp. OUS9 isolated from marine water

“…The bacterial culture DNA sample, which was amplified using the 16S rRNA primers main forward (5′-TCACGGAGTTT-GATCCTG-3) and reverse (5′-GCGGCTGCACGTA GTT-3′) primers, was then sequenced to generate 16S rRNA gene sequence. The resulting sequence was then evaluated using the BLAST similarity search program and contrasted with nucleotide in the “GenBank“ (NCBI) database ( Krishna et al, 2015 ). A phylogenetic tree was acquired with maximum probability demonstrating the evolutionary relationships between the chosen sequences ( Giovannoni et al, 1990 ).…”