Fermentative Production and Thermostability Characterization of Amylase fromAspergillusSpecies and Its Application Potential Evaluation in Desizing of Cotton Cloth

Abstract

The production of extracellular amylase was investigated employing our laboratory isolate, Aspergillus niger sp. MK 07 and effect of process variables on enzyme production, was studied in a fermentor. It was found that amylase production was maximum when the fermentor volume was maintained at 70%, rate of agitation at 250 rpm, air supply at 2.5 vvm, inoculum concentration of 10%, and a pH of 5.0. Highest enzyme production obtained under all optimized conditions was 1734 U/mL with sucrose as carbon substrate and corn steep liquor as nitrogen source. Enzyme purification studies by ammonium sulphate precipitation and Sephadex G-100 chromatography was evaluated for obtaining purified enzyme. Thermostability of amylase were evaluated with varying concentrations from 0.2 to 0.5 M concentrations of calcium chloride and the highest activity obtained was 3115 U/mL with 0.3 M calcium chloride at 55°C. Effect of temperature and pH on the activity of purified enzyme was evaluated and the purified enzyme showed an activity till 75°C and a pH of 6.5. Application potential of partially purified alpha amylase on desizing of cotton cloth was evaluated with varying enzyme concentrations from 50 to 500 U/mL and the highest desizing activity was found to be at 300 U/mL.

Amylase enzyme from Bacillus subtilis S8‐18: A potential desizing agent from the marine environment

“…An α‐amylase from Aspergillus sp. has been reported as an effective desizer for cotton fabrics ; the optimum enzyme concentration was found to be 300 U mL −1 Min −1 and further increase in the concentration had no significant effect on the desizing of the cloth. This might be because the appropriate enzyme to substrate ratio is essential to obtain optimal results .…”

Pilot-scale production of a highly thermostable α-amylase enzyme fromThermotoga petrophilacloned intoE. coliand its application as a desizer in textile industry

“…The total starch present in the grey fabric was calculated by hydrolyzing the starch with H 2 SO 4 (0.1 N). [33][34][35][36][37][38] Optimization of the cotton cloth desizing. The effect of different parameters, such as pH (4-9), temperature of incubation (50-100 C), enzyme concentration (50-300 U ml À1 min À1 ), time of incubation (30-120 min), effect of agitation rate (50-250 rpm), effect of various metal ions (10 mM) (Mg 2+ , Cu 2+ , Ca 2+ , Co 2+ , Na 1+ , K 1+ , NH 4…”

Amylase Production by some Local Fungal Isolates Using some Agricultural by -Products Via Solid State Fermentation

“…Potato dextrose starch agar medium (PDSA) (Chimata et al, 2011):Potatoes (sliced) 200.0g , dextros 10.0g , soluble starch 10.0g , agar 20.0g and distilled water 1000ml . pH was adjusted to 6.5 .…”

“…The fungal isolates were carried out using Potato dextrose starch agar medium (PDSA) of Chimata et al, (2011) .Fungal isolates were identified by the Regional Center of Mycology and Biotechnology ,Al Azhar University ,Cairo ,Egypt .It was grown at 30°C and stored at 4°C.…”