Amylase enzyme from Bacillus subtilis S8‐18: A potential desizing agent from the marine environment

Abstract

The present study is aimed at developing an economical medium for the production of α-amylase from Bacillus subtilis S8-18, a marine sediment isolate from Palk Bay, with various agricultural by-products that are cheap and rich in starch. These products include wheat bran, wheat husk, rice bran, rice husk, and potato peel and are used to replace soluble starch present in the Luria Bertani (LB) broth (synthetic medium). The rice husk was found to be the best to influence enzyme production significantly (61,186 IU mL⁻¹) when compared with the yield of 30,026 IU mL⁻¹ obtained by commercial starch. Hence, LB broth containing rice husk was considered an economical medium. In addition, the effect of various nutritional and physiological factors on enzyme production was also investigated. Furthermore, the desizing efficiency of α-amylases produced by synthetic and economical media was evaluated through various assays like reducing sugar estimation, weight loss assay, drop absorbency assay, scanning electron microscopy, and Fourier transform infrared analyses. In addition, a commercial α-amylase from B. subtilis was also used in desizing analyses for comparative purposes. It revealed that the α-amylase from the economical medium was as effective in desizing the cotton fabrics as that of the commercial enzyme and much superior to the enzyme produced through the synthetic medium.

α‐Amylase and cellulase production by novel halotolerant Bacillus sp.PM06 isolated from sugarcane pressmud

“…Similar results were observed in yeast cells Cryptococcus gilvescens for amylase activity [34] and in fungus Ganoderma applanatumMR-56 for cellulase activity [35]. Among the different nitrogen sources tested, ammonium chloride favored maximum growth, enzyme production of both α-amylase and cellulase compared with the control and other nitrogen sources [25]. Maximum enzyme production was observed at 48 H for both the enzymes [12,33].…”

“…The best carbon and nitrogen source for growth and enzyme production was identified. The strain showed maximum hydrolytic activity of α-amylase and cellulase when starch or CMC was used as a carbon source [25,33] and not produced with other carbon sources suggesting the inducible nature of Bacillus sp.PM06 enzyme. Similar results were observed in yeast cells Cryptococcus gilvescens for amylase activity [34] and in fungus Ganoderma applanatumMR-56 for cellulase activity [35].…”

“…To identify the best carbon source for growth and extracellular α-amylase and cellulase production of the isolate Bacillus sp.PM06, the basal medium was supplemented with (10 g/L) of different carbon sources (glucose, sucrose, mannose, fructose, maltose, soluble starch, and CMC). In order to study the effect of different nitrogen sources, basal medium with 10 g/L starch or CMC was supplemented with different organic nitrogen sources (1 g/L) (beef extract, tryptone, peptone, yeast extract, urea, and inorganic nitrogen sources like ammonium chloride, ammonium sulfate, and ammonium phosphate) [5,24,25]. At regular intervals, samples were collected for growth and enzyme production measurement.…”

Optimizing alpha-amylase from Bacillus amyloliquefaciens on bread waste for effective industrial wastewater treatment and textile desizing through response surface methodology

“…Consequently, enzymatic desizing has become a prevalent eco-friendly technique in textile processing 31 , 32 . It gently degrades starch-based sizing agents via enzymes like amylase, cellulase, maltase, and dextranase 33 without damaging the fabric, unlike harsh chemical desizing 29 . In this study, alpha-amylase concentration significantly impacted the desizing of textiles, with 347.5 U/mL exhibiting maximal efficacy.…”

Application of Response surface methodology in industrial wastewater treatment using Alpha-amylase from Bacillus amyloliquificiens grown on bread waste

“…Consequently, enzymatic desizing has become a prevalent ecofriendly technique in textile processing 31,32 . It gently degrades starch-based sizing agents via enzymes like amylase, cellulase, maltase, and dextranase 33 without damaging the fabric, unlike harsh chemical desizing 29 . In this study, alpha-amylase concentration significantly impacted the desizing of textiles, with 347.5 U/mL exhibiting maximal efficacy.…”