Fermentation of hexoses and pentoses from sugarcane bagasse hydrolysates into ethanol by Spathaspora hagerdaliae

Rech, Fernanda Roberta; Fontana, Roselei Claudete; Rosa, Carlos A.; Camassola, Marli; Ayub, Marco Antônio Záchia; Dillon, Aldo José Pinheiro

doi:10.1007/s00449-018-2016-y

Cited by 19 publications

(5 citation statements)

References 26 publications

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“…It seems that glucose repression is less strict in our strains than it is in S. cerevisiae , as it was previously reported (da Silva et al, 2019; Leite, Leite, Pereira, de Barros, & de Morais, 2016), allowing both sugars to be co‐consumed. This is an interesting result because the co‐utilization of glucose and d ‐xylose is not common, but it is essential for the conversion of lignocellulose to ethanol to be economically viable (Rech et al, 2019). For example, genetically modified strains of S. cerevisiae are able to consume d ‐xylose only after glucose utilization because of its repressive effect on the metabolism of secondary carbon sources (Scalcinati et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Fermentation profiles of the yeast Brettanomyces bruxellensis in d‐xylose and l‐arabinose aiming its application as a second‐generation ethanol producer

Silva

Ribeiro

Teles

et al. 2020

Yeast

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The yeast Brettanomyces bruxellensis is able to ferment the main sugars used in firstgeneration ethanol production. However, its employment in this industry is prohibitive because the ethanol productivity reached is significantly lower than the observed for Saccharomyces cerevisiae. On the other hand, a possible application of B. bruxellensis in the second-generation ethanol production has been suggested because this yeast is also able to use D-xylose and L-arabinose, the major pentoses released from lignocellulosic material. Although the latter application seems to be reasonable, it has been poorly explored. Therefore, we aimed to evaluate whether or not different industrial strains of B. bruxellensis are able to ferment D-xylose and Larabinose, both in aerobiosis and oxygen-limited conditions. Three out of nine tested strains were able to assimilate those sugars. When in aerobiosis, B. bruxellensis cells exclusively used them to support biomass formation, and no ethanol was produced. Moreover, whereas L-arabinose was not consumed under oxygen limitation, D-xylose was only slightly used, which resulted in low ethanol yield and productivity. In conclusion, our results showed that D-xylose and L-arabinose are not efficiently converted to ethanol by B. bruxellensis, most likely due to a redox imbalance in the assimilatory pathways of these sugars. Therefore, despite presenting other industrially relevant traits, the employment of B. bruxellensis in second-generation ethanol production depends on the development of genetic engineering strategies to overcome this metabolic bottleneck.

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Section: Resultsmentioning

confidence: 99%

Fermentation profiles of the yeast Brettanomyces bruxellensis in d‐xylose and l‐arabinose aiming its application as a second‐generation ethanol producer

Silva

Ribeiro

Teles

et al. 2020

Yeast

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“…passalidarum. 57,58 The genome mining resulted in two putative XR genes, where SpXYL1. strains wherein DXD and XLA are involved in gluconic acid production.…”

Section: Xylose Isomerase (Xi) Pathwaymentioning

confidence: 99%

Valorisation of xylose to renewable fuels and chemicals, an essential step in augmenting the commercial viability of lignocellulosic biorefineries

Narisetty

Cox

Bommareddy

et al. 2022

Sustainable Energy Fuels

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“…This use will depend on the microorganisms with capacity to metabolise this pentose. Several yeast species stand out as capable candidates for producing many of those products [ 18 , 19 , 20 , 21 ], such as the yeast Meyerozyma caribbica [ 22 ]. This is the new epithet of Pichia caribbica and the teleomorph of Candida fermentati (Division Ascomycota, class Saccharomycetes, order Saccharomycetales, family Debaryomycetaceae—Index Fungorum).…”

Section: Introductionmentioning

confidence: 99%

Meyerozyma caribbica Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

Alencar

Freitas

Guimarães

et al. 2023

JoF

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The production of fuels and other industrial products from renewable sources has intensified the search for new substrates or for the expansion of the use of substrates already in use, as well as the search for microorganisms with different metabolic capacities. In the present work, we isolated and tested a yeast from the soil of sugarcane irrigated with vinasse, that is, with high mineral content and acidic pH. The strain of Meyerozyma caribbica URM 8365 was able to ferment glucose, but the use of xylose occurred when some oxygenation was provided. However, some fermentation of xylose to ethanol in oxygen limitation also occurs if glucose was present. This strain was able to produce ethanol from molasses substrate with 76% efficiency, showing its tolerance to possible inhibitors. High ethanol production efficiencies were also observed in acidic hydrolysates of each bagasse, sorghum, and cactus pear biomass. Mixtures of these substrates were tested and the best composition was found for the use of excess plant biomass in supplementation of primary substrates. It was also possible to verify the production of xylitol from xylose when the acetic acid concentration is reduced. Finally, the proposed metabolic model allowed calculating how much of the xylose carbon can be directed to the production of ethanol and/or xylitol in the presence of glucose. With this, it is possible to design an industrial plant that combines the production of ethanol and/or xylitol using combinations of primary substrates with hydrolysates of their biomass.

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Fermentation of hexoses and pentoses from sugarcane bagasse hydrolysates into ethanol by Spathaspora hagerdaliae

Cited by 19 publications

References 26 publications

Fermentation profiles of the yeast Brettanomyces bruxellensis in d‐xylose and l‐arabinose aiming its application as a second‐generation ethanol producer

Fermentation profiles of the yeast Brettanomyces bruxellensis in d‐xylose and l‐arabinose aiming its application as a second‐generation ethanol producer

Valorisation of xylose to renewable fuels and chemicals, an essential step in augmenting the commercial viability of lignocellulosic biorefineries

Meyerozyma caribbica Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

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