Femtosecond laser multi-patterning of zirconia for screening of cell-surface interactions

Stanciuc, Ana-Maria; Flamant, Quentin; Sprecher, Christoph M.; Alini, Mauro; Anglada, M.; Peroglio, Marianna

doi:10.1016/j.jeurceramsoc.2017.08.019

Cited by 44 publications

(28 citation statements)

References 29 publications

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“…The type of tissue formed at this interface Femtosecond (fs) laser surface modification is used as a debris-or contaminant-free technique that can confer automatized, reproducible, fast time processed surfaces with increased roughness and stable characteristics. Due to low thermal loading and the ability to maintain the bulk properties of the material after fs laser processing, the disadvantages of other previously described surface modification techniques could be overcome [33], and high-quality microstructures and machining precision could be obtained as an exciting alternative to those obtained by conventional surface treatments of Zirconia surfaces [34][35][36][37][38][39][40].…”

Section: Introductionmentioning

confidence: 99%

Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro

Sima

Bonciu

Baciu³

et al. 2020

Nanomaterials

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Osseous implantology’s material requirements include a lack of potential for inducing allergic disorders and providing both functional and esthetic features for the patient’s benefit. Despite being bioinert, Zirconia ceramics have become a candidate of interest to be used as an alternative to titanium dental and cochlear bone-anchored hearing aid (BAHA) implants, implying the need for endowing the surface with biologically instructive properties by changing basic parameters such as surface texture. Within this context, we propose anisotropic and isotropic patterns (linear microgroove arrays, and superimposed crossline microgroove arrays, respectively) textured in zirconia substrates, as bioinstructive interfaces to guide the cytoskeletal organization of human mesenchymal stem cells (hMSCs). The designed textured micro-nano interfaces with either steep ridges and microgratings or curved edges, and nanoroughened walls obtained by direct femtosecond laser texturing are used to evaluate the hMSC response parameters and osteogenic differentiation to each topography. Our results show parallel micro line anisotropic surfaces are able to guide cell growth only for the steep surfaces, while the curved ones reduce the initial response and show the lowest osteogenic response. An improved osteogenic phenotype of hMSCs is obtained when grown onto isotropic grid/pillar-like patterns, showing an improved cell coverage and Ca/P ratio, with direct implications for BAHA prosthetic development, or other future applications in regenerating bone defects.

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Section: Introductionmentioning

confidence: 99%

Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro

Sima

Bonciu

Baciu³

et al. 2020

Nanomaterials

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“…On Zirconia, Stanciuc et al developed arrays of pits with different spacing, diameter and depth to evaluate human mesenchymal stem cells migration (hMSCs). Successful fabrication by femtosecond laser was achieved and the pits with 30 μm of diameter and 10 μm deep could be prone to induce stem cell commitment to the osteoblastic lineage based on the morphological evaluation of the cells [41].…”

Section: Discussionmentioning

confidence: 99%

Femtosecond laser microstructuring of alumina toughened zirconia for surface functionalization of dental implants

Carvalho

Grenho

Fernandes

et al. 2020

Ceramics International

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The continuous need for high-performance implants that can withstand mechanical loads while promoting implant integration into bone has focused recent research on the surface modification of hard ceramics. Their properties of biocompatibility, high mechanical and fatigue resistance and aesthetic color have contributed to its succefull applications in dentistry. Alumina toughened Zirconia (ATZ) has been gaining attention as a material for dental implants applications due to its advanced mechanical properties and minimal degradation at body temperature. Still, in order to improve tissue response to this bioinert material, additional modifications are desirable. Improving the surface functionality of this ceramic could lead to enhanced implant-tissue interaction and subsequently, a successful implant integration. In this work, microtopographies were developed on the surface of Alumina toughened Zirconia using an ultrafast laser methodology, aiming at improving the cellular response to this ceramic. Microscale grooves and grid-like geometries were produced on ATZ ceramics by femtosecond laser ablation, with a pulse width of 150 fs, wavelength of 800 nm and repetition rate of 1 kHz. The variation of surface topography, roughness, chemistry and wettability with different laser processing parameters was examined. Cell-surface interactions were evaluated for 7 days on both microstructured surfaces and a non-treated control with pre-osteoblasts, MC3T3-E1 cells. Both surface topographies showed to improve cell response, with increased metabolic activity when compared to the untreated control and modulating cell morphology up to 7 days. The obtained results suggest that femtosecond laser texturing may be a suitable non-contact methodology for creating tunable micro-scale surface topography on ATZ ceramics to enhance the biological response.

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“…A few studies have indicated that increased osteoblast proliferation on the nanostructured surfaces coincided with an increase in alkaline-phosphatase synthesis, increased Ca-containing mineral deposition, and higher immunostaining of osteocalcin and osteopontin [19].Recently, it has been demonstrated that osteointegration can be accelerated if the titanium surface is pre-treated to have a specific topography containing both micro-and nano-scale features [13]. In vivo experiments have shown that titanium and zirconium implants exhibit good osteointegration and that both elements have a high degree of bone-implant contact [20,21].The aim of this paper is to study the relationship between laser processing and osteoblast-like cell response on titanium-zirconium multilayer thin films. The formation of an ordered surface line-texture with micro and nanometer features was achieved by optimizing the laser parameters, including applied laser fluences, number of pulses, and scanning rate.…”

mentioning

confidence: 94%

“…Recently, it has been demonstrated that osteointegration can be accelerated if the titanium surface is pre-treated to have a specific topography containing both micro-and nano-scale features [13]. In vivo experiments have shown that titanium and zirconium implants exhibit good osteointegration and that both elements have a high degree of bone-implant contact [20,21].…”

mentioning

confidence: 99%

Laser-Assisted Surface Texturing of Ti/Zr Multilayers for Mesenchymal Stem Cell Response

et al. 2019

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The formation of an ordered surface texture with micro and nanometer features on Ti/Zr multilayers is studied for better understanding and improvement of cell integration. Nanocomposite in form 30×(Ti/Zr)/Si thin films was deposited by ion sputtering on Si substrate for biocompatibility investigation. Surface texturing by femtosecond laser processing made it possible to form the laser-induced periodic surface structure (LIPSS) in each laser-written line. At fluence slightly above the ablation threshold, beside the formation of low spatial frequency-LIPSS (LSFL) oriented perpendicular to the direction of the laser polarization, the laser-induced surface oxidation was achieved on the irradiated area. Intermixing between the Ti and Zr layers with the formation of alloy in the sub-surface region was attained during the laser processing. The surface of the Ti/Zr multilayer system with changed composition and topography was used to observe the effect of topography on the survival, adhesion and proliferation of the murine mesenchymal stem cells (MSCs). Confocal and SEM microscopy images showed that cell adhesion and their growth improve on these modified surfaces, with tendency of the cell orientation along of LIPSS in laser-written lines.Coatings 2019, 9, 854 2 of 12 originates in easy formation of outer Ti-oxide layer with a negative charge at physiological pH and protects against the metallic components dissolving in biological fluids [7]. One promising candidate for alloying is Zr, which act as a neutral element when dissolved in Ti and can improve mechanical properties of alloys [7]. From the biomedical point of view, Zr is fully soluble in both allotropic phases of Ti, improving the mechanical strength, corrosion resistance, and biocompatibility of Ti alloys [8]. Zirconium (Zr) has received special attention as an alloying element in Ti-based alloys, because it acts like non-toxic and non-allergenic elements, thereby avoiding stress shielding effects and implant failure [9]. Binary Ti-Zr alloy has exhibited a good combination of mechanical properties, with the advantage of easy manufacturing, in comparison to multicomponent, Ti-based alloys. Additionally, Zr with the lower elastic modulus (~80 GPa) can contribute to reducing stress shielding, which is the reason for the biomechanical incompatibility between the pure Ti-based implant and the bone [10].Currently, more sophisticated and versatile methods/tools for surface engineering and synthesis of nanoscale facilities for biomedical applications are needed. In traditional chemical and physical methods, although the microstructure can be controlled to some extent, the specific shape and size in some particular applications cannot be precisely controlled. Most of the traditional methods have disadvantages, such as low efficiency, high cost and difficult-to-machine. By contrast, laser processing can easily form and control desired complicated topography with high resolution and high economic efficiency [11,12]. Techniques based on laser surface modification have adva...

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Femtosecond laser multi-patterning of zirconia for screening of cell-surface interactions

Cited by 44 publications

References 29 publications

Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro

Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro

Femtosecond laser microstructuring of alumina toughened zirconia for surface functionalization of dental implants

Laser-Assisted Surface Texturing of Ti/Zr Multilayers for Mesenchymal Stem Cell Response

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