Feline infectious peritonitis: isolation of a coronavirus

OReilly, Kathleen; Fishman, Baruch; Hitchcock, L M

doi:10.1136/vr.104.15.348

Cited by 30 publications

(24 citation statements)

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“…Fcwf ceils and a strain of FIPV adapted to this line of macrophage-like cells were studied since they constitute a reproducible in vitro system. FIPV has been propagated also in other cell lines, however, which have not been qualified as macrophage-like (O'Reilly et al, 1979;Black, 1980;Hitchcock et al, 1981 ;Evermann et al, 1981), and some not even of feline origin (H. E. L. Jacobse-Geels & M. C. Horzinek, unpublished observations). Pedersen (1976) was the first to report growth of FIPV in explanted autochthonous peritoneal cells and Weiss & Scott (1981) demonstrated viral antigen in cultivated buffy coat cells of experimentally infected cats.…”

Section: Discussionmentioning

confidence: 99%

Expression of Feline Infectious Peritonitis Coronavirus Antigens on the Surface of Feline Macrophage-like Cells

Jacobse-Geels

Horzinek

1983

Journal of General Virology

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SUMMARYGrowth of feline infectious peritonitis virus in a continuous feline celt line is described and evidence for the macrophage-like character of these cells is presented. Under one-step growth conditions, cytopathic changes and giant cell formation were observed 12 h after infection; more than 99~ of the virus remained cell-associated 15 h after infection. Viral proteins at the surface of infected cells were detected by immunofluorescence. The exposed antigens were localized on four proteins with molecular weights of 225.5K, 175K, 138K and 25K using radioiodination followed by immunoprecipitation. Another viral polypeptide of 44K (the nucleocapsid protein) was only labelled when the cell membranes had been disrupted. Expression of viral antigens on the cell surface may be a significant factor in the immune pathogenesis of feline infectious peritonitis.

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Section: Discussionmentioning

confidence: 99%

Expression of Feline Infectious Peritonitis Coronavirus Antigens on the Surface of Feline Macrophage-like Cells

Jacobse-Geels

Horzinek

1983

Journal of General Virology

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“…The disease FIP was first described in 1963 as a syndrome in cats characterized by immune-mediated vasculitis and pyogranulomatous inflammatory reactions [24]. In 1978, a virus was identified as the etiologic agent, and in 1979, it was classified as a coronavirus labeled ''feline infectious peritonitis virus'' (FIPV) [25]. FIP has become an increasingly important disease for veterinarians and must now be considered to account for most infectious disease-related deaths in pet cats, thus taking over this title in recent years from feline leukemia virus (FeLV) infection, which is decreasing in prevalence and importance.…”

Section: Etiologymentioning

confidence: 99%

Feline infectious peritonitis

Hartmann

2005

Veterinary Clinics of North America: Small Animal Practice

145

251

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The article discusses feline infectious peritonitis (FIP), an important disease frequently seen in veterinary practice. FIP causes many problems to the veterinarian as it can be difficult to definitively diagnose the disease, as there is no effective treatment, and as prophylactic interventions are not very successful. Although intense research has created a lot of new knowledge about this disease in the last years, there are still many unanswered questions. The objective of this article is to review recent knowledge and to increase understanding of the complex pathogenesis of FIP.

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“…Pedersen & Boyle, 1980;Ward, 1970;Hoshino & Scott, 1978;Osterhaus et al, 1978). O'Reilly et al (1979) succeeded in culturing FIP virus in monolayer cultures of feline embryonic lung (FEL) cells, and a complete account of the in vitro culture method has been reported (Hitchcock et al, 1981). This paper describes in detail, for the first time, the ultrastructure of FIP virus in cultured FEL cells.…”

Section: Introductionmentioning

confidence: 99%

“…FIP virus is fastidious in its choice of host cells and has until recently (Evermann et al, 1981;Hitchcock et al, 1981;Black, 1980;O'Reilly et al, 1979) defied attempts to grow it in tissue culture. For this reason FIP virus has been extensively studied only in animals and in organ cultures (e.g.…”

Section: Introductionmentioning

confidence: 99%

The Ultrastructure of Feline Infectious Peritonitis Virus in Feline Embryonic Lung Cells

Beesley

Hitchcock

1982

Journal of General Virology

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SUMMARYThe ultrastructure of feline infectious peritonitis virus in cultured feline embryonic lung cells is reported. Feline embryonic lung cells were infected with feline infectious peritonitis virus and studied by transmission electron microscopy. The virus was not apparent in the cultured cells until 24 h after infection when it occurred in the endoplasmic reticulum, perinuclear space, Golgi apparatus, free in the cytoplasm, in large vacuoles in the cytoplasm and outside the cell membrane. The virus possessed typical coronavirus morphology and was produced by budding into the endoplasmic reticulum. There was no evidence to indicate that this virus budded through the cell membrane. Multinucleate giant cells were formed by infection of the cultured cells with the virus. The host cells were destroyed by the virus and phagocytosed by apparently healthy cells.

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Feline infectious peritonitis: isolation of a coronavirus

Cited by 30 publications

References 0 publications

Expression of Feline Infectious Peritonitis Coronavirus Antigens on the Surface of Feline Macrophage-like Cells

Expression of Feline Infectious Peritonitis Coronavirus Antigens on the Surface of Feline Macrophage-like Cells

Feline infectious peritonitis

The Ultrastructure of Feline Infectious Peritonitis Virus in Feline Embryonic Lung Cells

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