Expression of Feline Infectious Peritonitis Coronavirus Antigens on the Surface of Feline Macrophage-like Cells

Jacobse-Geels, H. E. L.; Horzinek, Marian C.

doi:10.1099/0022-1317-64-9-1859

Cited by 48 publications

(41 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It differs from serotype II FCoV in that it hardly grows in tissue culture (Jacobse-Geels & Horzinek, 1983); however, oral administration of faeces or gut homogenates from shedding cats leads to intestinal infection and monocyte-associated viraemia (Meli et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

Sites of feline coronavirus persistence in healthy cats

Kipar

Meli

Baptiste

et al. 2010

Journal of General Virology

122

149

View full text Add to dashboard Cite

Feline coronavirus (FCoV) is transmitted via the faecal-oral route and primarily infects enterocytes, but subsequently spreads by monocyte-associated viraemia. In some infected cats, virulent virus mutants induce feline infectious peritonitis (FIP), a fatal systemic disease that can develop in association with viraemia. Persistently infected, healthy carriers are believed to be important in the epidemiology of FIP, as they represent a constant source of FCoV, shed either persistently or intermittently in faeces. So far, the sites of virus persistence have not been determined definitely. The purpose of this study was to examine virus distribution and viral load in organs and gut compartments of specified-pathogen-free cats, orally infected with non-virulent type I FCoV, over different time periods and with or without detectable viraemia. The colon was identified as the major site of FCoV persistence and probable source for recurrent shedding, but the virus was shown also to persist in several other organs, mainly in tissue macrophages. These might represent additional sources for recurrent viraemia.

show abstract

Section: Resultsmentioning

confidence: 99%

Sites of feline coronavirus persistence in healthy cats

Kipar

Meli

Baptiste

et al. 2010

Journal of General Virology

122

149

View full text Add to dashboard Cite

show abstract

“…FIPV infection, however, can only be mediated by fAPN but not by porcine or human APN (Delmas et al, 1993 ;Kolb et al, 1997). Consequently, the feline cell lines CRFK and (Ringold et al, 1975), Felis catus whole fetus cells (FCWF ; Jacobse-Geels & Horzinek, 1983) and MRC5 human embryonic lung fibroblasts (all cultivated as described previously ; Kolb et al, 1997) were infected with FIPV (strain 79-1146, propagated on FCWF cells) at an m.o.i. of 5 and labelled metabolically with […”

Section: Author For Correspondence : Andreas Kolb Present Address : mentioning

confidence: 99%

Characterization of determinants involved in the feline infectious peritonitis virus receptor function of feline aminopeptidase N.

Hegyi

Kolb²

1998

Journal of General Virology

View full text Add to dashboard Cite

Feline aminopeptidase N (fAPN) is a major cell surface receptor for feline infectious peritonitis virus (FIPV), transmissible gastroenteritis virus (TGEV), human coronavirus 229E (HCV 229E) and canine coronavirus (CCV). By using chimeric molecules assembled from porcine, human and feline APN we have analysed the determinants involved in the coronavirus receptor function of fAPN. Our results show that amino acids 670-840 of fAPN are critically involved in its FIPV and TGEV receptor function whereas amino acids 135-297 are essential for the HCV 229E receptor function. We also demonstrate that a chimeric molecule assembled from human and porcine APN is able to act as a receptor for FIPV. This is surprising as neither human nor porcine APN by themselves mediate FIPV infection. These results suggest that different determinants in the APN protein are involved in mediating the coronavirus receptor function.The Coronaviridae are enveloped viruses with a large nonsegmented RNA genome of positive polarity. On the basis of serological and genetic data they are separated into three different groups (Siddell, 1995). Some members of group 1 [i.e. transmissible gastroenteritis virus (TGEV), feline infectious peritonitis virus (FIPV) and human coronavirus (HCV) 229E] have been demonstrated to utilize aminopeptidase N (APN) as cell surface receptor (Delmas et al., 1992 ;Yeager et al., 1992 ;Tresnan et al., 1996). APN is a type II glycoprotein, belongs to the family of membrane-bound metalloproteases (Olsen et al., 1988) and is expressed in a variety of tissues (Delmas et al., 1994).Although human APN (hAPN) and porcine APN (pAPN) have a sequence similarity of 78 % at the amino acid level,

show abstract

“…Serotype I FCoV isolates have been numerically superior [22] and predominantly distributed among cats [9]. These differences in antigenic properties are generally consistent with viral growth ability in vitro [2,22]; serotype II FCoVs propagate more readily than serotype I FCoVs in feline cell cultures such as Felis catus whole fetus-4 (fcwf-4) [13] and Crandell feline kidney (CRFK) cells. In addition, serotype II FCoVs are more efficiently neutralized by antiserum against canine coronavirus (CCV), indicating their closer antigenic relatedness with CCV.…”

Section: Methodsmentioning

confidence: 72%

Antigenic and Plaque Variations of Serotype II Feline Infectious Peritonitis Coronaviruses.

Mochizuki

Mitsutake

Miyanohara

et al. 1997

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Three feline coronavirus (FCoV) isolates KUK-H, M91-266, and M91-267 were examined to elucidate their biological and antigenic properties as well as disease potential in cats. Immune stainings of virus-infected cells by using FCoV type-specific monoclonal antibodies indicated that their antigenic specificity was serotype II. However, antigenic variations among these serotype II FCoVs were detected by neutralization assay with hyperimmune antisera against FCoVs and canine coronaviruses, and with experimentally infected cat sera; there were two subtypes in serotype II FCoVs. The isolates efficiently grew in fcwf-4 cell culture showing lytic CPE enough to form distinct plaques; when measured 48 hr after infection, plaque sizes of both M91-266 and M91-267 were approximately 1 mm in diameter, and a mixture of small (less than 1mm in diameter) and large (approximately 3 mm in diameter) plaques were produced in the case of KUK-H. Strains KUK-H, M91-266 and M91-267 produced feline infectious peritonitis (FIP) in 50%, 67% and 89% of experimentally inoculated kittens, respectively. Furthermore, 80% of the kittens inoculated with the small plaque former of KUK-H developed FIP accompanied by more prominent clinical signs as well as pathological changes when compared with 28.6% of kittens inoculated with the large plaque former. These results suggest that serotype II FIPVs producing smaller size of plaques are more virulent than those producing larger size of plaques. -KEY WORDS: coronavirus, feline, feline infectious peritonitis, plaque, virulence.J. Vet. Med. Sci. 59(4): 253-258, 1997 serotype II FCoV has arisen by recombination between serotype I FCoV and CCV. In the present paper, three serotype II FCoV isolates from clinical FIP cases were examined in respects of antigenic property and in vivo pathogenic virulence especially in relation to plaque characteristics. Discussion was also made regarding relationships between plaque properties and virulence of coronaviruses in animals. MATERIALS AND METHODSViruses and cell culture: KUK-H strain was isolated in 1987 by using CRFK cells (ATCC CCL94), and both M91-266 and M91-267 strains were isolated in 1991 by using fcwf-4 cells. All derived from the spleen samples taken at the postmortem examination of effusive form FIP field cases. Stock viruses were prepared after further several passages in fcwf-4 cell culture. Strain C3663, a local isolate from an effusive form FIP case, was used as a reference serotype I FCoV. The cells were cultured as described previously [18].Plaque assay: The stock viruses were characterized by plaque assay, and then the virus was plaque-purified for further experiments. After serial tenfold virus dilutions, 0.2 ml of each dilution was inoculated onto fcwf-4 cell monolayer in 60-mm plastic plates. The plates were incubated at 37°C for 1 hr, and 4 ml of an overlay medium was added. The plates were further incubated at 37°C for 2 or 3 days and then stained with either an overlay medium According to the recent concept about feline coronaviru...

show abstract

Expression of Feline Infectious Peritonitis Coronavirus Antigens on the Surface of Feline Macrophage-like Cells

Cited by 48 publications

References 22 publications

Sites of feline coronavirus persistence in healthy cats

Sites of feline coronavirus persistence in healthy cats

Characterization of determinants involved in the feline infectious peritonitis virus receptor function of feline aminopeptidase N.

Antigenic and Plaque Variations of Serotype II Feline Infectious Peritonitis Coronaviruses.

Contact Info

Product

Resources

About