The Ultrastructure of Feline Infectious Peritonitis Virus in Feline Embryonic Lung Cells

Beesley, Julian E.; Hitchcock, L M

doi:10.1099/0022-1317-59-1-23

Cited by 8 publications

(6 citation statements)

References 8 publications

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“…In some cases, the progeny coronavirus does not appear until 24 hr p.i. [Beesley and Hitchcock, 1982]. This phenomenon might cause the acute syndrome of SARS.…”

Section: Discussionmentioning

confidence: 99%

The life cycle of SARS coronavirus in Vero E6 cells

Zhang

Cui

Huang

et al. 2004

Journal of Medical Virology

121

113

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The aim of the study was to establish the life cycle of severe acute respiratory syndrome-associated coronavirus (SARS CoV) in host cells and determine the pathogenesis of SARS. Vero E6 cells (African green monkey kidney cells) were inoculated with SARS coronavirus for 3, 7, 24, 48, and 72 hr, respectively, and were observed under electron microscope. It was found that the SARS coronavirus entered the cells through membrane fusion instead of endocytosis, and then the nucleocapsids assembled in the RER and matured by budding into the smooth vesicles, which were derived from the Golgi apparatus. The smooth vesicles fused with the cell membrane, and the mature particles were released. A special phenomenon was that some virus-like particles appeared in the nucleus. We propose a scheme of the life cycle of SARS coronavirus and discuss the mechanism of its replication in Vero E6 cells.

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“…In some cases, the progeny coronavirus does not appear until 24 hr p.i. [Beesley and Hitchcock, 1982]. This phenomenon might cause the acute syndrome of SARS.…”

Section: Discussionmentioning

confidence: 99%

The life cycle of SARS coronavirus in Vero E6 cells

Zhang

Cui

Huang

et al. 2004

Journal of Medical Virology

121

113

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“…The reason for these negative results is unknown; the number of virions in the mucosa may be small or their presence of short duration. In contrast, several studies have been published of coronavirus-infected cells in in vitro systems Beesley and Hitchcock 1982;Chasey and Alexander 1976;David-Ferreira and Manaker 1965;Everman et al 1989;Hamre et al 1967;McIntosh et al 1967; Os- et al 1971), or in an in vivo system (Takeuchi et al 1976), although none to my knowledge on a ciliated epithelium.…”

Section: Discussionmentioning

confidence: 99%

“…This scheme of virus replication differs somewhat from that seen in studies of cell cultures. Coronavirusinfected cells are reported to contain virions in the perinuclear cisterna and in the endoplasmic reticulum, where they also replicate (Becket et al 1967;Beesley and Hitchcock 1982;Chasey and Alexander 1976;Hamre et al 1967;Takeuchi et al 1976). These compartments were free from virions in the present case and budding was seen only in the Golgi apparatus.…”

Section: Discussionmentioning

confidence: 99%

“…Replicating coronaviruses have been studied in some cell cultures, such as human fetal diploid lung cells (Oshiro et al 1971), human WI-38 cells Hamre et al 1967), canine kidney cells (Takeuchi et al 1976), feline kidney cells (Everman et al 1989), feline embryonic lung cells (Beesley and Hitchcock 1982), and chick kidney cells (Chasey and Alexander 1976); these cells are non-ciliated. Virions are recorded in several components within these cells: endoplasmic reticulum, perinuclear cisterna, Golgi apparatus, lysosomes, and cytoplasmic vesicles.…”

Section: Introductionmentioning

confidence: 99%

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Ultrastructure of human nasal epithelium during an episode of coronavirus infection

Afzelius

1994

Vichows Archiv A Pathol Anat

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The nasal epithelium from a young girl was examined by electron microscopy and found to be infected by coronavirus. Virions are seen within and outside the ciliated cells, but not outside or within the goblet cells or other cells of the nasal mucosa. Some virions are located near the microvilli, others in pockets in the apical cell membrane. The cytoplasm contains many small vesicles with a single virion, large apical vesicles containing hundreds of virions, and lysosome-like cytosomes with a moderate number of virions. Some virus-like particles devoid of an electron-dense interior are seen both in the cytosomes and extracellularly. Virus budding was observed in the Golgi apparatus but nowhere else in the cell. The ciliated cells seem not to be destroyed by the viruses, although in many cases the cilia are withdrawn into the cell body. The loss of cilia is likely to cause rhinorrhoea.

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“…These results are consistent with prior data on the limited in vitro growth potential of the virus in a variety of cell types and the tendency for the immunofluorescence patterns to be confined primarily to the perinuclear region of the infected cells. The electron microscopy of the cytolytic coronaviruses, FECV 79-1683 and FIPV 79-1146 revealed similarities in terms of multiple immature and mature virus particles in the cytoplasmic cisternae and the accumulation of mature virions with peplomers in and at the vicinity of the cell surface (Chasey and Alexander, 1976;Beesley and Hitchcock, 1982).…”

Section: Discussionmentioning

confidence: 99%

Comparative features of a coronavirus isolated from a cheetah with feline infectious peritonitis

et al. 1989

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A coronavirus which was isolated from a cheetah (Acinonyx jubatus) that succumbed to feline infectious peritonitis was characterized in vitro. The virus was determined to be highly cell-associated with Crandell feline kidney (CrFK) cells and was routinely maintained as a persistent infection (CrFK 83-4497). The cheetah coronavirus was compared with other members of the feline coronavirus group including the feline enteric coronavirus (FECV) 79-1683 and the feline infectious peritonitis viruses (FIPV), 79-1146, and UCD-1. The cheetah coronavirus was demonstrated to have a restricted host-cell range with limited cytopathic effect. Indirect immunofluorescence with antisera to FIPV UCD-1 revealed the concentration of viral antigens in the perinuclear region of cells infected with the cheetah coronavirus. Ultrastructural studies of the cheetah coronavirus indicated a limited number of immature viral particles within cytoplasmic vesicles and at the cell surface. This was in contrast to electron microscopy results of FECV 79-1683 and FIPV 79-1146, which had numerous mature virus particles within the cytoplasmic vesicles, as well as at the cell surface. The cheetah coronavirus was tentatively placed in the feline coronavirus family based upon its antigenic reactivity by immunofluorescence; however, the possibility that it represents a unique coronavirus of cheetahs should not be dismissed without further analyses at the host and genomic levels.

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The Ultrastructure of Feline Infectious Peritonitis Virus in Feline Embryonic Lung Cells

Cited by 8 publications

References 8 publications

The life cycle of SARS coronavirus in Vero E6 cells

The life cycle of SARS coronavirus in Vero E6 cells

Ultrastructure of human nasal epithelium during an episode of coronavirus infection

Comparative features of a coronavirus isolated from a cheetah with feline infectious peritonitis

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