Fc receptors of liver sinusoidal endothelium in normal rats and humans

Enhancement of colorectal tumor targeting using a novel biparatopic monoclonal antibody against carcinoembryonic antigen in experimental radioimmunoguided surgery

“…Increased accretion to the liver in fragment-and biparatopic MAb-pretreated mice was explained by antigen-antibody and antigen-mediated. 16,17 High kidney activity in F(abЈ) 2 -and FabЈ-pretreated mice appears to occur from tubular reabsorption after glomerular filtration. 5 Biparatopic MAb was newly constructed to improve tumor targeting and tumor retention time of MAb.…”

“…1 One clinical trial of RIGS using 99m Tc-PR1A3 demonstrated a lower sensitivity (66%) probably from the significant background activity of 99m Tc. 17 Although the sensitivity has been raised with various immune probes in RIGS, limited specificity without specific tumor marker remains to be determined. A newly designed biparatopic anti-CEA MAb would appear to solve these problems.…”

Kupffer cell depletionin vivoresults in preferential elimination of IgG aggregates and immune complexes via specific Fc receptors on rat liver endothelial cells

“…Studies in humans and experimental models have established that the liver is primarily responsible for the removal of circulating IC from the circulation (Rifai & Mannik, 1984;Skogh et al, 1985;Lobatto et al, 1987;Halma et al, 1989;Rifai et al, 1989;Bogers et al, 1989Bogers et al, , 1990. Within the liver, the liver macrophages (Kupffer cells, KC) are considered to be responsible for IC clearance (Benacerraf, Sebestyen & Cooper, 1959; Rifai & Mannik, 1984;Bogers et al, 1990 Very recently it has been reported that endothelial cells (EC) in rat and human liver sections are able to bind IgG-IC in vitro (Muro et al, 1987). Furthermore, in tiro studies reported binding of IgG-IC to liver EC (Skogh et al, 1985;Van Der Laan-Klamer et al, 1985, 1986bMuro etal., 1987).…”

Establishment of a novel monoclonal antibody, SE-1, which specifically reacts with rat hepatic sinusoidal endothelial cells.

“…Therefore, we suggest that the antigen recognized by SE-1 is not directly related to the immunological response of endothelial cells. The immunohistochemical staining pattern of SE-1 in the liver, i.e., positive for HSE cells but negative for portal and central vascular endothelial cells, is similar to that of the Fc receptor reported by Muro et al (19). However, unlike the Fc receptor, Kupffer cells were negative for SE-1, suggesting a difference between these two antigens.…”