Establishment of a novel monoclonal antibody, SE-1, which specifically reacts with rat hepatic sinusoidal endothelial cells.

Ohmura, Tosei; Enomoto, Katsuhiko; Satoh, Hiroshi; Sawada, Norimasa; Mori, Makoto

doi:10.1177/41.8.8331290

Cited by 56 publications

(42 citation statements)

References 7 publications

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“…SECs were isolated from the fraction by the immunomagnetic method using 4.5-m-diameter magnetic beads (Dynabeads, CELLection Pan Mouse IgG kit; Dynal, Oslo, Norway) conjugated with SE-1 monoclonal antibody, 23 which specifically recognizes an antigen expressed in rat SECs. As described previously, this method enabled us to isolate SECs with high purity (98%), which was better than that obtained by the conventional Percoll method.…”

Section: Isolation and Culture Of Secsmentioning

confidence: 99%

Maintenance of Bad Phosphorylation Prevents Apoptosis of Rat Hepatic Sinusoidal Endothelial Cells in Vitro and in Vivo

Ohi

Nishikawa

Tokairin

et al. 2006

The American Journal of Pathology

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To elucidate the mechanism of apoptosis of liver sinusoidal endothelial cells (SECs), we examined the phosphorylation status of Bad and its upstream signaling molecules during apoptosis in culture and after ischemia-reperfusion injury. Rat SECs were isolated by the immunomagnetic method, and 2 days after culture, most SECs underwent apoptosis, which was associated with decreased tyrosine phosphorylation of cellular proteins. Addition of orthovanadate (OV), a protein tyrosine phosphatase inhibitor, sustained cellular protein phosphorylation and strongly inhibited apoptosis. Bad was dephosphorylated at Ser-112 and Ser-136 during apoptosis, but the phosphorylation status of Bad was maintained in the presence of OV. OV activated the Akt, extracellular signalregulated protein kinase, and p38 mitogen-activated protein kinase pathways, which are involved in Bad phosphorylation. In the absence of OV, depletion of Bad by RNA interference conferred resistance to apoptosis. Hepatic injury after ischemia-reperfusion was alleviated by OV treatment, with significant inhibition of SEC apoptosis. SEC apoptosis in vivo was associated with dephosphorylation of Bad, Akt, and extracellular signal-regulated protein kinase, which was blocked by OV treatment. Our data suggest that maintenance of Bad phosphorylation is important in the prevention of SEC apoptosis and that the anti-apoptotic property of OV might have therapeutic utility.

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Section: Isolation and Culture Of Secsmentioning

confidence: 99%

Maintenance of Bad Phosphorylation Prevents Apoptosis of Rat Hepatic Sinusoidal Endothelial Cells in Vitro and in Vivo

Ohi

Nishikawa

Tokairin

et al. 2006

The American Journal of Pathology

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“…The reaction products were found along the surface of plasma membrane of SEC (Fig. 3a), but the positive reaction at this stage was weaker than that of the adult rat liver as reported previously (12) and sometimes showed segmental distribution on the plasma membrane of SEC. The SE-1 positive SEC (Fig.…”

Section: Results Smentioning

confidence: 81%

“…The monoclonal antibody, SE-1, has been established as an antibody that reacts especially with SECof the adult rat liver but not with any other type of vascular endothelial cell (12). While the nature and function of SE-1 antigen have yet to be clarified, the antigen is considered to be related to the specific function of SEC in adult rat livers.…”

Section: Discussionmentioning

confidence: 99%

“…room temperature. Specific reaction products were detected in 0.02% diaminobenzidine-4HCl (Wako Pure Chemical, Osaka, Japan) and 0.0045% H2O2 solution as previously described (12). They were post-fixed in 1% osmium solution, dehydrated in a series of concentrated ethanol, and embedded in epoxy resin using propylene oxide.…”

Section: Animalsmentioning

confidence: 99%

“…45 kD) expressed only in rat liver SEC and in no other type of endothelial cell (7,12). Although the nature of SE-1 antigen has yet to be elucidated, the expression of SE-1 antigen has been considered to be related to some specific function of SEC.…”

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confidence: 99%

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Analysis of the Sinusoidal Endothelial Cell Organization during the Developmental Stage of the Fetal Rat Liver Using a Sinusoidal Endothelial Cell Specific Antibody, SE-1.

Morita

Wang

Watanabe

et al. 1998

Cell Struct. Funct.

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Keywords: sinusoidal endothelial cells/SE-1 antibody/fetal rat liver ABSTRACT.The sinusoid organization during the development of fetal rat livers was studied using a SE-1 antibody, which we have previously established as a specific monoclonal antibody against rat sinusoidal endothelial cell (SEC). Expression and localization of the SE-1 antigen in the liver tissues of 13-to 21-day-old fetuses were immunofluorescently and immunoelectron microscopically examined. The first positive fluorescence was observed in the immature liver of 15-day-old fetuses. The initial positive staining was randomly distributed in the liver parenchyma and showed no direct relation to the large vessels which may be derived from the fetal vitelline veins. The positive linear staining increased in number and connected with each other during the course of development. The SE-1 staining pattern and the sinusoidal arrangement became similar to those of the adult liver after 20th day of gestation. Immunoelectron microscopically, the immature SEC showed a weak positive reaction for the SE-1 antigen at their membraneand was observed together with immature hepatocytes and hematopoietic cells in the 15-day-old fetal liver. Along with the liver development, SEC formed a sinusoid structure closely associated with hepatocytes and came to strongly express the SE-1 antigen. These results indicate that the organization of the hepatic sinusoid may start at around 15th day of the gestation and occurs randomly in the fetal liver parenchyma. It is also suggested that the expression of SE-1 antigen is possibly regulated by the intimate association with hepatocytes.The liver sinusoid is a specialized vascular system which consists of endothelial cells with characteristic morphological features, such as open fenestration and lack of basal lamina (10, 16). These features are thought to facilitate the active exchangeof various metabolic substances between blood and hepatocytes through the space of Disse. Although the mechanisms of appearance and maintenance of these morphological featuresare not yet known, recent studies have suggested that these structures are regulated by hepatocyte related factors or by the extracellular matrix (8, 9).Structure and function of SEC have been well studied in adult rat livers as described above. However, only a few studies have reported on the structural changes in SEC during development of the fetal rat liver (1, 2, ll). Barbera-Guillem et al. (2) reported that the number and size of the fenestration of SECdiffer greatly depending on their zonal location in the liver and stage of development. However, the details in the sinusoidal organization in fetal livers have yet to be well established.Wepreviously established a novel monoclonal antibody, SE-1, which specifically recognized an antigen (M.W. 45 kD) expressed only in rat liver SEC and in no other type of endothelial cell (7,12). Although the nature of SE-1 antigen has yet to be elucidated, the expression of SE-1 antigen has been considered to be related to some specific fun...

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The Liver Sinusoidal Endothelial Cell

Sørensen

Smedsrød

2020

The Liver

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Establishment of a novel monoclonal antibody, SE-1, which specifically reacts with rat hepatic sinusoidal endothelial cells.

Cited by 56 publications

References 7 publications

Maintenance of Bad Phosphorylation Prevents Apoptosis of Rat Hepatic Sinusoidal Endothelial Cells in Vitro and in Vivo

Maintenance of Bad Phosphorylation Prevents Apoptosis of Rat Hepatic Sinusoidal Endothelial Cells in Vitro and in Vivo

Analysis of the Sinusoidal Endothelial Cell Organization during the Developmental Stage of the Fetal Rat Liver Using a Sinusoidal Endothelial Cell Specific Antibody, SE-1.

The Liver Sinusoidal Endothelial Cell

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