Fatty acids in the lipids ofDrosophila heads: Effects of visual mutants, carotenoid deprivation and dietary fatty acids

Stark, William S.; Lin, Teng‐Nan; Brackhahn, David; Christianson, J. Scott; Sun, Grace Y.

doi:10.1007/bf02536321

Cited by 28 publications

(18 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been shown clearly in the present studies that feeding Drosophila on the two different diets resulted in significant modulation in mitochondrial phospholipid fatty acyl composition. This is consistent with previous results 36 .…”

Section: Discussionsupporting

confidence: 94%

“…The result is that increasing the level of one fatty acid in the diet of an animal does not lead to high levels of just that fatty acid in the animal's membrane lipids, but it also affects the composition of other fatty acids, resulting in a fairly constant level of ratio of unsaturated to saturated fatty acids. This homeostatic control has been reported in Drosophila 36 and was apparent in the present studies.…”

Section: Discussionsupporting

confidence: 88%

“…Dietary fatty acid manipulation is a wellestablished method known to alter membrane phospholipid fatty acyl profiles in several organisms. The effect of dietary fatty acids on cellular and sub-cellular membrane lipid fatty acid composition has been widely reported in cell cultures 16 , bacteria 35 , yeast 25 , insects 36 , birds 30 and mammals 32 . Dietary lipid modulates membrane lipid composition by two main mechanisms: (i) By providing the fatty acids available to be incorporated into membrane phospholipids, or (ii) By providing the precursors for other membrane lipid fatty acids.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Dietary fatty acids alter mitochondrial phospholipid fatty acyl composition and proton leak in Drosophila melanogaster

Ocloo¹

2010

Biokemistri

View full text Add to dashboard Cite

Two groups of fruit flies (Drosophila melanogaster) were maintained on different diets.Mitochondria were isolated, proton leak was measured and phospholipid fatty acid composition determined. Mitochondria from flies fed on corn-base meal (containing high amounts of polyunsaturated fatty acids, 18:2(n-6) and 18:3(n-3) and low amounts of monounsaturated fatty acid, 18:1(n-9)) contained more polyunsaturated fatty acids in their membranes than mitochondria from flies fed on yeast-base meal (containing less amount of polyunsaturates and high amount of monounsaturates). The more polyunsaturated mitochondria were leakier to protons than the more monounsaturated mitochondria.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Dietary fatty acids alter mitochondrial phospholipid fatty acyl composition and proton leak in Drosophila melanogaster

Ocloo¹

2010

Biokemistri

View full text Add to dashboard Cite

show abstract

“…Our results suggest that the diet of the larva contributes significantly to the high content of polyunsaturated fatty acids in the butterfly. Dietary effects on the fatty acid composition of tissues has been shown in several other insect species (9)(10)(11)(12). In silkworm, Unni et al (13) observed that the fatty acid composition of the diet affected its growth.…”

Section: Discussionmentioning

confidence: 99%

The predominance of polyunsaturated fatty acids in the butterfly Morpho peleides before and after metamorphosis

Wang

Lin

Bolewicz

et al. 2006

Journal of Lipid Research

View full text Add to dashboard Cite

We hypothesized that the polyunsaturated fatty acids of the butterfly were probably derived from the diet and that there might be a great loss of body fat during metamorphosis. To substantiate these hypotheses, we analyzed the fatty acid composition and content of the diet, the larva, and the butterfly Morpho peleides. Both the diet and the tissues of the larva and butterfly had a high concentration of polyunsaturated fatty acids. In the diet, linolenic acid accounted for 19% and linoleic acid for 8% of total fatty acids. In the larva, almost 60% of the total fatty acids were polyunsaturated: linolenic acid predominated at 42% of total fatty acids, and linoleic acid was at 17%. In the butterfly, linolenic acid represented 36% and linoleic acid represented 11% of total fatty acids. The larva had a much higher total fatty acid content than the butterfly (20.2 vs. 6.9 mg). Our data indicate that the transformation from larva to butterfly during metamorphosis drastically decreased the total fatty acid content. There was bioenhancement of polyunsaturated fatty acids from the diet to the larva and butterfly. Insects and vertebrates share many common metabolic pathways. Although lipid metabolism in vertebrates has been well studied, there are fewer studies in insects. Insects may be useful models that can facilitate our general understanding of biology (1). For most insects, there is a dietary requirement for polyunsaturated fatty acids (2, 3). Using an analysis of the fatty acid composition of seven insect orders, Thompson (4) identified possible phylogenetic trends. It was also observed that in the order Lepidoptera, most of the species had a high content of polyunsaturated fatty acids. The linolenic acid of the 49 species analyzed varied from 0.1% to 51.0% of total fatty acids, with a mean of 22.2%. These trends provided insights into potential relationships between insect orders. The fatty acid composition of many insect species has been analyzed in the past (5, 6). However, in view of the multitude of insect species that exist, most knowledge of the fatty acid compositions of insects is derived from studies of a relatively small number of insect species.The butterfly of the order Lepidoptera undergoes metamorphosis from larva to butterfly. The larva feed on the leaves of the plants and then spin a cocoon. The butterfly feeds on flower nectar, which is available later in the year. We found no information about the fatty acid composition and fat content of the butterfly before and after metamorphosis and no information about how the diet of the larva might influence its fatty acid composition.On a recent trip, we visited the Butterfly Farm at Chaa Creek, Belize, in Central America. Chaa Creek is a tourist attraction and hostel for travelers in Belize. As we have developed an interest in the evolutionary patterns of fatty acids, having previously studied snails and slugs (7), we saw the potentialities of butterfly research and discussed our ideas with the scientific director, Mike Green. We decided to collab...

show abstract

“…In the Drosophila, there were changes in fatty acid composition such that the peroxidation index of lipids increased with age and flight activity and was associated with an increased susceptibility to lipid peroxidation (215). The fatty acid composition of phospholipids from the heads of wild-type Drosophila shows them to have short-chain PUFA but lack long-chain PUFA (338,379). Although there are considerable data for fatty acid composition of invertebrates in general, they are almost never presented in relationship to aging or maximum life span of the particular species.…”

Section: Ectothermic Vertebrates and Invertebratesmentioning

confidence: 99%

Life and Death: Metabolic Rate, Membrane Composition, and Life Span of Animals

Hulbert¹,

Pamplona²,

Buffenstein

et al. 2007

Physiological Reviews

749

725

View full text Add to dashboard Cite

Maximum life span differences among animal species exceed life span variation achieved by experimental manipulation by orders of magnitude. The differences in the characteristic maximum life span of species was initially proposed to be due to variation in mass-specific rate of metabolism. This is called the rate-of-living theory of aging and lies at the base of the oxidative-stress theory of aging, currently the most generally accepted explanation of aging. However, the rate-of-living theory of aging while helpful is not completely adequate in explaining the maximum life span. Recently, it has been discovered that the fatty acid composition of cell membranes varies systematically between species, and this underlies the variation in their metabolic rate. When combined with the fact that 1) the products of lipid peroxidation are powerful reactive molecular species, and 2) that fatty acids differ dramatically in their susceptibility to peroxidation, membrane fatty acid composition provides a mechanistic explanation of the variation in maximum life span among animal species. When the connection between metabolic rate and life span was first proposed a century ago, it was not known that membrane composition varies between species. Many of the exceptions to the rate-of-living theory appear explicable when the particular membrane fatty acid composition is considered for each case. Here we review the links between metabolic rate and maximum life span of mammals and birds as well as the linking role of membrane fatty acid composition in determining the maximum life span. The more limited information for ectothermic animals and treatments that extend life span (e.g., caloric restriction) are also reviewed.

show abstract

Fatty acids in the lipids ofDrosophila heads: Effects of visual mutants, carotenoid deprivation and dietary fatty acids

Cited by 28 publications

References 14 publications

Dietary fatty acids alter mitochondrial phospholipid fatty acyl composition and proton leak in <i>Drosophila melanogaster</i>

Dietary fatty acids alter mitochondrial phospholipid fatty acyl composition and proton leak in <i>Drosophila melanogaster</i>

The predominance of polyunsaturated fatty acids in the butterfly Morpho peleides before and after metamorphosis

Life and Death: Metabolic Rate, Membrane Composition, and Life Span of Animals

Contact Info

Product

Resources

About