Fatty Acid Composition of Cultivated Edible Mushroom Lentinus Tuberregium Vkjm24 (Hm060586)

Gowri, Shymala S

doi:10.20959/wjpr201710-9368

Cited by 1 publication

(1 citation statement)

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“…After that, by dehydration, the smaller but most abundant fragment ion m / z 111.0086 [M – 80 − H] - was produced from the fragment m / z 129.0192, or by the secondary decarboxylation at a higher collision energy, the fragment m / z 82.0295 [M – 62 − H] - was formed. In this way, other 22 organic acids were identified, including mevalonic acid (peak 7) [57], methylsuccinic acid (peak 8) [58], 3-methylglutaric acid (peak 9) [59], suberic acid (peak 26) [60], 3-tertbutyladipic acid (peak 38) [61], 9-hydroxy-10,14-octadecadien-12-ynoic acid (peak 43) [62], 9,12,13-trihydroxy-15-octadecenoic acid (peak 48) [63], moroctic acid (peak 49) [64], 5,8-dihydroxy-9,12-octadecadienoic acid (peak 55) [63], 3-hydroxy-(4,2-hydroxy-6-methyl)-2 heptanyl benzoic acid (peak 57) [65], porrigenic acid (peak 58) [66], 12-oxo-phytodienoic acid (peak 60) [67], 3-methyl-5-pentyl-2-furanundecanoic acid (peak 63) [68], 10-hydroxy-8,12-octadecadienoic acid (peak 67), α-eleostearic acid (peak 68) [69], 8-oxo-9-octadecenoic acid (peak 69) [70], 6,7-epoxystearic acid (peak 71) [63], 9-oxo-octadecadienoic acid (peak 73) [71], 2,3-seco-2,3-dicarboxyplatanic acid (peak 74) [72], diroleuton (peak 83) [73], linoleic acid (peak 93) [74], and 1-(hydroxymethyl)-2[(1-oxohexadecyl)oxy]ethyl-ester-9,12-heptadecadienoic acid (peak 108) [75]. All these organic acids were identified from the genus of Sparassis for the first time.…”

Section: Resultsmentioning

confidence: 99%

Rapid Characterization of Chemical Components in Edible Mushroom Sparassis crispa by UPLC-Orbitrap MS Analysis and Potential Inhibitory Effects on Allergic Rhinitis

et al. 2019

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Sparassis crispa is a kind of edible fungus widely grows in the north temperate zone, which shows various medicinal properties. Due to the complexity of chemical constitutes of this species, few investigations have acquired a comprehensive configuration for the chemical profile of it. In this study, a strategy based on ultra-high performance liquid chromatography (UPLC) combined with Orbitrap mass spectrometer (MS) was established for rapidly characterizing various chemical components in S. crispa. Through the summarized MS/MS fragmentation patterns of reference compounds and systematic identification strategy, a total of 110 components attributed to six categories were identified for the first time. Moreover, allergic rhinitis (AR) is a worldwide inflammatory disease seriously affecting human health, and the development of drugs to treat AR has been a topic of interest. It has been reported that the extracts of S. crispa showed obvious inhibitory effects on degranulation of mast cell- and allergen-induced IgE and proinflammatory mediators, but the active components and specific mechanism were still not clear. Src family kinases (SFKs) participate in the initial stage of allergy occurrence, which are considered the targets of AR treatment. Herein, on the basis of that self-built chemical database, virtual screening was applied to predict the potential SFKs inhibitors in S. crispa, using known crystal structures of Hck, Lyn, Fyn, and Syk as receptors, followed by the anti-inflammatory activity evaluation for screened hits by intracellular calcium mobilization assay. As results, sparoside A was directly confirmed to have strong anti-inflammatory activity with an IC50 value of 5.06 ± 0.60 μM. This study provides a useful elucidation for the chemical composition of S. crispa, and demonstrated its potential inhibitory effects on AR, which could promote the research and development of effective agents from natural resources.

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