Fatty acid composition and mycolic acid pattern of some chromogenic mycobacteria

Valero-Guillén, Pedro L.; Pacheco, F.; Martin-Luengo, F.

doi:10.1111/j.1365-2672.1985.tb03309.x

Cited by 19 publications

(15 citation statements)

References 16 publications

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“…As determined by TLC, M. alvei contains a-mycolates and 0-1 methoxymycolates. Since as determined by gas chromatography M. alvei produces lipid profiles similar to those of the other rapidly growing nonphotochromogenic mycobacteria (18,20,25,45), analysis of the mycolic acid pattern allows a clear distinction between M. alvei and the previously described Mycobactenurn species. Recently, we have found that M. porcinurn, M. senegalense, and some strains of M. fortuitum and "Mycobactenurn peregrinum" also contain appreciable amounts of 0-1 rnethoxymycolate, but in these species this mycolate is always accompanied by an epoxy- (Table 3) (26,27,31).…”

Section: Resultsmentioning

confidence: 99%

“…Six types of mycolates have been recognized in this way; these mycolates are mycolate types I and I1 (long and short mycolates with no additional oxygenated functions), I11 (methoxymycolates), IV (ketomycolates), V (epoxymycolates), and VI (dicarboxymycolates). Mycolate TLC patterns for the different mycobacterial species have been published previously either in catalogs or in previous studies (4, 21,30,32,33,45).…”

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confidence: 99%

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Mycobacterium alvei sp. nov.

Ausina

Luquin²,

Barceló³

et al. 1992

International Journal of Systematic Bacteriology

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A new species of rapidly growing, nonphotochromogenic mycobacteria, Mycobacterium alvei, is described. The inclusion of this organism in the genus Mycobacterium is based on its acid fastness, its mycolate pattern, and its G+C content. A study of six strains showed that they form a homogeneous group with an internal phenotypic similarity value of 97 f 2.22%. DNA relatedness studies showed that the six M. ulvei strains which we studied form a single DNA hybridization group which is less than 4% related to 14 other species of the genus Mycobacterium; the AT,,, values determined for the strains which exhibited higher levels of DNA homology were all greater than 7.9OC. A lipid analysis showed that tuberculostearic acid was present. Docosanoic and tetracosanoic acid methyl esters were detected as mycolic acid cleavage products. All six isolates which we tested contained a-mycolic acids and relatively large amounts of a new kind of mycolic acid containing a methoxy group at w-1 position, a characteristic that has not been described previously in mycobacteria. Strain CR-21 is the type strain; a culture of this strain has been deposited in the Collection Nationale

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Mycobacterium alvei sp. nov.

Ausina

Luquin²,

Barceló³

et al. 1992

International Journal of Systematic Bacteriology

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“…Since the 1950s, GLC and TLC have been the chromatographic techniques most widely used to analyse the composition of mycobacterial mycolic acids as methyl esters (Daffe! et al, 1983 ;Minnikin et al, 1984 ;Luquin et al, 1991 ;Valero-Guille! n et al, 1985).…”

Section: Discussionmentioning

confidence: 99%

“…The mycolate patterns obtained upon separation of mycolic acid methyl esters by TLC have been widely used in the classification and identification of mycobacteria (Daffe! et al, 1983 ;Minnikin et al, 1984 ;Luquin et al, 1991 ;Valero-Guille! n et al, 1985).…”

Section: Abbreviations : Ei-ms Electron-impact Ms ; Fab-ms Fast-atomentioning

confidence: 99%

The HPLC-double-cluster pattern of some Mycobacterium gordonae strains is due to their dicarboxy-mycolate content

et al. 2002

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The mycolic acids of several strains of Mycobacterium gordonae were examined by chromatographic and spectroscopic techniques. Both HPLC and TLC revealed two patterns of mycolates among the M. gordonae strains studied. As determined by TLC, one pattern was composed of α-, methoxy-and keto-mycolates ; the other was composed of these mycolates plus an additional component, which was identified as dicarboxy-mycolates. The dicarboxymycolates were only found in those M. gordonae strains that displayed a so-called HPLC-double-cluster pattern. Detailed structural analyses of the dicarboxy-mycolates indicated that these compounds contained predominantly 61-65 carbon atoms (C 63 was the major component) and a trans-1,2-disubstituted cyclopropane ring. Thus, the dicarboxy-mycolate content of strains of M. gordonae determines their HPLC pattern. In spite of the differences in their HPLC patterns, and although they belonged to different PCR-restriction length polymorphism clusters, all of the M. gordonae strains examined in this study were closely related on the basis of the structural features of their α-, keto-and methoxy-mycolates ; the predominant α-mycolates contained two cis-1,2-disubstituted cyclopropane rings, the major keto-mycolates contained a trans-1,2-disubstituted cyclopropane ring and the methoxy-mycolates contained one cis-or one trans-1,2-disubstituted cyclopropane ring. It is noteworthy that the strains containing dicarboxymycolates also displayed significant amounts of α-mycolates that contained one cis-1,2-disubstituted cyclopropane ring and one cis double bond. The results obtained in this study demonstrate heterogeneity among M. gordonae strains.

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“…structures of mycolic acids have proved to be valuable criteria in the classification and identification of members of these taxa (1,2,6,11,15,(19)(20)(21)(22)(23)28). Two types of nonoxygenated mycolates (with ethylenic double bounds or cyclopropane rings in their longest chains [the mero-chains]) and four types of mycolates with oxygenated functions in their mero-chains (methoxymycolates, ketomycolates, epoxymycolates, and wax ester mycolates) have been identified in the genus Mycobacterium (1-3, 5-9, 13, 18, 25, 26).…”

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Distribution of a Novel Mycolic Acid in Species of the Genus Mycobacterium

Luquin

Lanéelle²,

Ausina³

et al. 1991

International Journal of Systematic Bacteriology

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We found that Mycobacterium porcinum ATCC 33776T (T = type strain) contains a new kind of mycolic acid with a methoxy group at the w-1 position. This mycolic acid was identified by comparing it with the previously described methoxymycolic acids. The patterns of mycolic acid methyl esters from 418 strains belonging to 44 species of mycobacteria were studied by using thin-layer chromatography. In addition to M . porcinum ATCC 33776T, representative strains of M. porcinum, Mycobacterium fortuitum, "Mycobacterium peregrinum," Mycobacterium senegalense, and a recently isolated Mycobacterium sp. contained appreciable amounts of the newly described mycolic acid.Mycolic acids are characteristic 2-branched, 3-hydroxy acids with very long chains (up to 90 carbon atoms) and are found only in mycobacteria, nocardiae, rhodococci, corynebacteria, and related taxa (1,12,14,20). Differences in the structures of mycolic acids have proved to be valuable criteria in the classification and identification of members of these taxa (1,2,6,11,15,(19)(20)(21)(22)(23)28). Two types of nonoxygenated mycolates (with ethylenic double bounds or cyclopropane rings in their longest chains [the mero-chains]) and four types of mycolates with oxygenated functions in their mero-chains (methoxymycolates, ketomycolates, epoxymycolates, and wax ester mycolates) have been identified in the genus Mycobacterium (1-3, 5-9, 13, 18, 25, 26). Recently, a fifth type of oxygenated mycolate was found in a Mycobacterium sp. isolated from the environment (17). Structural analysis showed that this mycolate contained a methoxy group at the 0-1 position (instead of the 0-17 or 0-18 position, as in previously described methoxymycolates) and two double bounds in the long mero-chain (instead of the one double bond found in the previously described mycolates with oxygenated groups). Thin-layer chromatography showed that the Mycobacteriurn porcinum type strain contains, in addition to a-and epoxymycolates (16), a more polar component with the same chromatographic behavior as the w-1 methoxymycolate mentioned above. In this study we found that the structure of the most polar mycolic acid of the M . porcinum type strain is the same as the structure of the recently describe 0-1 methoxymycolate, and the patterns of the mycolates of representative strains of 44 mycobacterial species were investigated to assess the distribution of the novel 0-1 methoxymycolate. MATERIALS AND METHODSThe sources of the 418 strains selected for this study are shown in Table 1. All of the strains were identified by conventional methods (27,29,30). For isolation of lipid components, bacteria were cultivated on plates of Middlebrook 7H10 agar and incubated at 37°C in 5% CO, for 21 days, except as noted below. Strains of Mycobacterium fallax, Mycobacterium marinum, Mycobacterium gadium, * Corresponding author.Mycobacterium aichiense, Mycobacterium chubuense, Mycobacterium obuense, and Mycobacterium rhodesiae were incubated at 30"C, and Mycobacterium thermoresistibile strains were incubated at 42°C.A...

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Fatty acid composition and mycolic acid pattern of some chromogenic mycobacteria

Cited by 19 publications

References 16 publications

Mycobacterium alvei sp. nov.

Mycobacterium alvei sp. nov.

The HPLC-double-cluster pattern of some Mycobacterium gordonae strains is due to their dicarboxy-mycolate content

Distribution of a Novel Mycolic Acid in Species of the Genus Mycobacterium

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