Fast determination of myo-inositol in milk powder by ultra high performance liquid chromatography coupled to tandem mass spectrometry

Flores, María Isabel Alarcón; Moreno, José L.; Frenich, Antonia Garrido; Vidal, José Luis Martı́nez

doi:10.1016/j.foodchem.2011.05.045

Cited by 20 publications

(3 citation statements)

References 25 publications

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“…Myo-inositol, as a conditionally essential nutrient, has been shown to have chemopreventive and chemotherapeutic properties in cancer, cataracts, neuropathy, and nephropathy (Grases et al, 2002;Rizvi et al, 2006;Liu et al, 2009;Schlemmer et al, 2009;Corrado et al, 2011). Additionally, myo-inositol is a compound necessary for babies, and when this substance cannot be administered through breast milk, it must be sup- plied in the diet by inositol-enriched milk or formula (Hallman et al, 1987;Flores et al, 2011;Woollard et al, 2014;Indyk et al, 2016). In this study, myo-inositol was also identified in donkey milk.…”

Section: Discussionmentioning

confidence: 63%

Comparative metabolomics analysis of donkey colostrum and mature milk using ultra-high-performance liquid tandem chromatography quadrupole time-of-flight mass spectrometry

Liang

Kang

Zheng

et al. 2020

Journal of Dairy Science

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Donkey milk has been widely shown to be an ideal substitute for human milk because of its similar composition. However, alterations to the composition of donkey milk during lactation have not been well studied. In this study, untargeted metabolomics with ultra-high-performance liquid tandem chromatography quadrupole time-of-flight mass spectrometry were used to analyze and compare the metabolites in donkey colostrum (DC) and mature milk (DMM). Two hundred seventy metabolites were characterized in both DC and DMM. Fifty-two of the metabolites in the DC were significantly different from those in the DMM; 8 were downregulated and 44 were upregulated. This demonstrated that the composition of the donkey milk changed with lactation. Additionally, the interactions and metabolic pathways were further analyzed to explore the mechanisms that altered the milk during lactation. Our results provide comprehensive insights into the alterations in donkey milk during lactation. The results will aid in future investigations into the nutrition of donkey milk and provide practical information for the dairy industry.

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Section: Discussionmentioning

confidence: 63%

Comparative metabolomics analysis of donkey colostrum and mature milk using ultra-high-performance liquid tandem chromatography quadrupole time-of-flight mass spectrometry

Liang

Kang

Zheng

et al. 2020

Journal of Dairy Science

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“…According to a study published in the journal, the polarity of the existing ESI mass detector was set when the inositol assay was analyzed in the positive ( Flores et al ., 2011 ) or negative mode ( Kim et al ., 2012 ). In this study, the test was carried out in both positive and negative modes to determine a suitable polarity for the ESI mass detector used in the inositol LC-MS/MS assays.…”

Section: Resultsmentioning

confidence: 99%

Development Rapid Analytical Methods for Inositol as a Trace Component by HPLC and LC-MS/MS in Infant Formula

Shin

Park

Kim

et al. 2015

Korean Journal for Food Science of Animal Resources

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A rapid and simple analytical method, using liquid chromatography tandem mass spectrometry (LC-MS/MS), was developed to detect myo-inositol (MI) in infant formulas. For protein removal: acid hydrolysis and lipid removal through organic solvent extraction. The operating conditions for instrumental analysis were determined based on previously reported analogous methods that used LC-MS/MS. Quantitative analysis was used for the detection limit test, infant formula recovery test, and standard reference material (SRM) 1849a to verify the validity of our LC-MS/MS analytical method, which was developed to quantify MI. For validation, the results of our method were compared with the results of quantitative analyses of certified values. The test results showed that the limit of detection was 0.05 mg/L, the limit of quantitation was 0.17 mg/L, and the method detection limit was 17 mg/kg. The recovery test exhibited a recovery between 98.07-98.43% and a relative standard deviation between 1.93-2.74%. Therefore, the result values were good. Additionally, SRM 1849a was measured to have an MI content of 401.84 mg/kg and recovery of 98.25%, which is comparable to the median certified value of 409 mg/kg. From the aforementioned results, we judged that the instrumental analysis conditions and preparation method used in this study were valid. The rapid analytical method developed herein could be implemented in many laboratories that seek to save time and labor.

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“…The analysis of free myo-inositol in foods and biological tissues has generally been facilitated following a simple protein precipitation step in advance of end-point analysis. Methods targeting the aggregate of free and bound forms have used acidic or alkaline hydrolysis followed by traditional microbiological assay (83), enzymatic assay (84), GC subsequent to previous derivatization (85-88), HPLC or high-performance anion-exchange chromatography (HPAEC) techniques using UV, evaporative light-scattering detection (ELSD), or pulsed amperometric detection (PAD) (82, 89-94),and HPLC or UHPLC coupled with MS(95)(96)(97). A review has summarized the techniques that are applicable for the analysis of inositol and related compounds, albeit specific to soybean tissues(98).…”

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confidence: 99%

Current Methods for the Analysis of Selected Novel Nutrients in Infant Formulas and Adult Nutritionals

Gill

Indyk

Woollard³

2016

Journal of AOAC INTERNATIONAL

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Infant formula is designed to provide the human infant with a sole source of nutrition and it is intended to imitate breast milk. In recent years, advances in the science of infant nutrition have led to an increasing number of novel ingredients that are supplemented into infant formula. As the list of these nutritionally important nutrients is lengthy, this review summarizes contemporary analytical methods that have been applied to a representative selection (lutein, carnitine, choline, nucleotides, inositol, taurine, sialic acid, gangliosides, triacylglycerides, oligosaccharides, α-lactalbumin, and lactoferrin).

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Fast determination of myo-inositol in milk powder by ultra high performance liquid chromatography coupled to tandem mass spectrometry

Cited by 20 publications

References 25 publications

Comparative metabolomics analysis of donkey colostrum and mature milk using ultra-high-performance liquid tandem chromatography quadrupole time-of-flight mass spectrometry

Comparative metabolomics analysis of donkey colostrum and mature milk using ultra-high-performance liquid tandem chromatography quadrupole time-of-flight mass spectrometry

Development Rapid Analytical Methods for Inositol as a Trace Component by HPLC and LC-MS/MS in Infant Formula

Current Methods for the Analysis of Selected Novel Nutrients in Infant Formulas and Adult Nutritionals

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