Fast detection of atrazine in corn using thermometric biosensors

Qie, Zhiwei; Ning, Baoan; Liu, Ming; Bai, Jintao; Peng, Yuan; Song, Nan; Lv, Zhiqiang; Wang, Ying; Sun, Siming; Su, Xuan; Zhang, Yihong; Gao, Zhixian

doi:10.1039/c3an00490b

Cited by 14 publications

(5 citation statements)

References 11 publications

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“…Meanwhile the simultaneously generated heat can be precisely and continuously detected with high resolution thermistor sensors ( Ramanathan and Danielsson, 2001 ; Zhou et al., 2013 ). The thermistor sensor converts the heat generated by the reaction into a temperature signal ( Xie et al., 2000 ; Qie et al., 2013 ). In an adiabatic system, if all the heat released is used to increase the temperature of the reaction system, the following formula can be used:…”

Section: Methodsmentioning

confidence: 99%

Activity fingerprinting of AMR β-lactamase towards a fast and accurate diagnosis

Song,

Sun,

Wang

et al. 2023

Front. Cell. Infect. Microbiol.

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Antibiotic resistance has become a serious threat to global public health and economic development. Rapid and accurate identification of a patient status for antimicrobial resistance (AMR) are urgently needed in clinical diagnosis. Here we describe the development of an assay method for activity fingerprinting of AMR β-lactamases using panels of 7 β-lactam antibiotics in 35 min. New Deli Metallo β-lactamase-1 (NDM-1) and penicillinase were demonstrated as two different classes of β-lactamases. The panel consisted of three classes of antibiotics, including: penicillins (penicillin G, piperacillin), cephalosporins (cefepime, ceftriaxone, cefazolin) and carbapenems (meropenem and imipenem). The assay employed a scheme combines the catalytic reaction of AMR β-lactamases on antibiotic substrates with a flow-injected thermometric biosensor that allows the direct detection of the heat generated from the enzymatic catalysis, and eliminates the need for custom substrates and multiple detection schemes. In order to differentiate classes of β-lactamases, characterization of the enzyme activity under different catalytic condition, such as, buffer composition, ion strength and pH were investigated. This assay could provide a tool for fast diagnosis of patient AMR status which makes possible for the future accurate treatment with selected antibiotics.

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Section: Methodsmentioning

confidence: 99%

Activity fingerprinting of AMR β-lactamase towards a fast and accurate diagnosis

Song,

Sun,

Wang

et al. 2023

Front. Cell. Infect. Microbiol.

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“…Ferentions et al 100 developed a novel artificial neural network combined with a bioelectric cellular biosensor to detect and classify correctly the presence of investigated pesticide groups with an overall success rate of 83.6%. Qie et al 101 reported that thermometric biosensors evidently simplify sample pretreatment, and exhibit a potentially powerful capability for fast quantitative analysis of pesticide residues. The short assay time per sample of biosensors makes them suitable for the fast detection of large-scale samples.…”

Section: New Rapid Detection Methods Of the Safety Monitoring Of Tcm mentioning

confidence: 99%

Safety issues and new rapid detection methods in traditional Chinese medicinal materials

Wang¹,

Kong²,

Yang³

et al. 2015

Acta Pharmaceutica Sinica B

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The safety of traditional Chinese medicine (TCM) is a major strategic issue that involves human health. With the continuous improvement in disease prevention and treatment, the export of TCM and its related products has increased dramatically in China. However, the frequent safety issues of Chinese medicine have become the ‘bottleneck’ impeding the modernization of TCM. It was proved that mycotoxins seriously affect TCM safety; the pesticide residues of TCM are a key problem in TCM international trade; adulterants have also been detected, which is related to market circulation. These three factors have greatly affected TCM safety. In this study, fast, highly effective, economically-feasible and accurate detection methods concerning TCM safety issues were reviewed, especially on the authenticity, mycotoxins and pesticide residues of medicinal materials.

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“…13 Unfortunately, the assay possessed limited sensitivity. We suspected that this was primarily due to inefficiency and/or instability in the formation of ATZ-antibody complexes.…”

Section: Introductionmentioning

confidence: 99%

“…We have previously reported the development of a FIA-TELISA assay for detecting ATZ in corn using an enzyme thermistor (ET). 13 Unfortunately, the assay possessed limited sensitivity. We suspected that this was primarily due to inefficiency and/or instability in the formation of ATZ-antibody complexes.…”

Section: Introductionmentioning

confidence: 99%

Sensitive detection of atrazine in tap water using TELISA

Qie¹,

Bai²,

Xie

et al. 2015

Analyst

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A highly sensitive flow injection analysis (FIA)-based thermal enzyme-linked immunoassay, TELISA, was developed for the rapid detection of atrazine (ATZ) in tap water. ATZ and β-lactamase-labeled ATZ were employed in a competitive immunoassay using a monoclonal antibody (mAb). After the off-column liquid-phase competition, the mAb was captured on the Protein G Sepharose™ 4 Fast Flow (PGSFF) column support material. Injected β-lactamase substrate ampicillin was degraded by the column-bound ATZ-β-lactamase, generating a detectable heat signal. Several assay parameters were optimized, including substrate concentration, flow rates and regeneration conditions, as well as the mAb and ATZ-β dilution ratios and concentrations. The assay linear range was 0.73-4.83 ng mL(-1) with a detection limit of 0.66 ng mL(-1). An entire heat signal requires 10 min for generation, and the cycle time is less than 40 min. The results were reproducible and stable. ATZ-spiked tap water samples exhibited a recovery rate of 103%-116%, which correlated with the UHPLC-MS/MS measurements. We attributed this significant increase in sensitivity over our previously published work to the following factors: (i) the capture of already-formed immune complexes on the column via immobilized Protein G, which eliminated chemical immobilization of the antibody; (ii) off-column preincubation allows the formation of immune complexes under nearly ideal conditions; and (iii) multiple buffers can be used to, in one case, enhance immune-complex formation and in the other to maximize enzymatic activity. Furthermore, the scheme creates a universal assay platform in which sensing is performed in the off-column incubation and detection after capture in the enzyme thermistor (ET) detector, which opens up the possibility of detecting any antigen for which antibodies were available.

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Fast detection of atrazine in corn using thermometric biosensors

Cited by 14 publications

References 11 publications

Activity fingerprinting of AMR β-lactamase towards a fast and accurate diagnosis

Activity fingerprinting of AMR β-lactamase towards a fast and accurate diagnosis

Safety issues and new rapid detection methods in traditional Chinese medicinal materials

Sensitive detection of atrazine in tap water using TELISA

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