Safety issues and new rapid detection methods in traditional Chinese medicinal materials

Wang, Lili; Kong, Weijun; Yang, Meihua; Han, Jianping; Chen, Shilin

doi:10.1016/j.apsb.2014.12.005

Cited by 41 publications

(17 citation statements)

References 69 publications

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“…Therefore, use of authentic species is essential to maintain the growth and development of medicinal plant sectors. DNA diagnostics have become the gold standard for scientific validation of herbal products 14 , 24 . Previously, random amplification polymorphic DNA (RAPD) markers were developed to authenticate Shankhpushpi species 25 as well as for genetic diversity analysis 1 , 26 .…”

Section: Resultsmentioning

confidence: 99%

Internal transcribed spacer guided multiplex PCR for species identification of Convolvulus prostratus and Evolvulus alsinoides

Sharma

Shrivastava

2016

Acta Pharmaceutica Sinica B

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Shankhpushpi is a reputed drug from an Indian system of medicine for treating mental disorders and enhancing memory. Two herbs, namely Convolvulus prostratus Forssk. and Evolvulus alsinoides (L.) L., are commonly known as Shankhpushpi. Ambiguous vernacular identity can affect the scientific validity of the Shankpushpi-based herbal drug therapy. In the present investigation, a novel and sensitive multiplex PCR method based on polymorphism in the internal transcribed spacer (ITS) region was developed to establish the molecular identity of C. prostratus and E. alsinoides. DNA was isolated and the ITS region was amplified, sequenced and assembled. Sequences were aligned to identify variable nucleotides in order to develop plant-specific primers. Primers were validated in singleplex reactions and eventually a multiplex assay was developed. This assay was tested for sensitivity and validated by amplifying DNA isolated from the simulated blended powdered plant material. Primers developed for C. prostratus resulted into a 200 bp amplicon and 596 bp for E. alsinoides. The assay was found to be sensitive enough for amplification of low quantities of DNA. The method can detect 10% of the mixing of plants with each other in blended material. This PCR assay can be used for rapid botanical identification of Shankhpushpi plant materials and will improve evidence-based herbal drug therapy.

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Section: Resultsmentioning

confidence: 99%

Internal transcribed spacer guided multiplex PCR for species identification of Convolvulus prostratus and Evolvulus alsinoides

Sharma

Shrivastava

2016

Acta Pharmaceutica Sinica B

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“…Environmental pollution, non-standardized planting, processing and storage, and other factors contribute to excessive accumulation of exogenous toxic residues. The residues of heavy metals and harmful elements [62] , pesticide residues [63] , mycotoxins [64] , and pigment residues [65] are considered to be the most primary exogenous toxic residues and seriously affect the safety of TCMs [66] . The testing methods and maximum residue limits (MRLs) were set in the pharmacopoeia of each country.…”

Section: Elements Of “Ivarious” Standard Systemmentioning

confidence: 99%

A holistic strategy for quality and safety control of traditional Chinese medicines by the “iVarious” standard system

Chen

Sun

Yuan

et al. 2017

Journal of Pharmaceutical Analysis

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An effective quality control system is the key to ensuring the quality, safety and efficacy of traditional Chinese medicines (TCMs). However, the current quality standard research lacks the top-design and systematic design, mostly based on specific technologies or evaluation methods. To resolve the challenges and questions of quality control of TCMs, a brand-new quality standard system, named “iVarious”, was proposed. The system comprises eight elements in a modular format. Meaning of every element was specifically illustrated via corresponding research instances. Furthermore, frankincense study was taken as an example for demonstrating standards and research process, based on the “iVarious” system. This system highlighted a holistic strategy for effectiveness, security, integrity and systematization of quality and safety control standards of TCMs. The establishment of “iVarious” integrates multi-disciplinary technologies and progressive methods, basis elements and key points of standard construction. The system provides a novel idea and technological demonstration for regulation establishment of TCMs quality standards.

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“…There are increasing applications of Chinese herbal medicines (CHMs) for the prevention and treatment of various illnesses, and in parallel, the increased use has been accompanied by an emerging concern regarding the safety of CHMs ( Zeng and Jiang, 2010 ; Willamson et al, 2013 ; Wang et al, 2015 ). Cases of severe adverse effects caused by CHMs, particularly hepatotoxicity, have been frequently reported over the past few decades ( Teschke et al, 2014 ; Chan et al, 2015 ; Coghlan et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

The Modulatory Role of CYP3A4 in Dictamnine-Induced Hepatotoxicity

Jiang

Dong-sheng

et al. 2018

Front. Pharmacol.

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Dictamni Cortex (DC) has been reported to be associated with acute hepatitis in clinic and may lead to a selective sub-chronic hepatotoxicity in rats. Nevertheless, the potent toxic ingredient and the underlying mechanism remain unknown. Dictamnine (DTN), the main alkaloid from DC, possesses a furan ring which was suspected of being responsible for hepatotoxicity via metabolic activation primarily by CYP3A4. Herein, the present study aimed to evaluate the role of CYP3A4 in DTN-induced liver injury. The in vitro results showed that the EC50 values in primary human hepatocytes (PHH), L02, HepG2 and NIH3T3 cells were correlated with the CYP3A4 expression levels in corresponding cells. Furthermore, the toxicity was increased in CYP3A4-induced PHH by rifampicin, and CYP3A4 over-expressed (OE) HepG2 and L02 cells. Contrarily, the cytotoxicity was decreased in CYP3A4-inhibited PHH and CYP3A4 OE HepG2 and L02 cells inhibited by ketoconazole (KTZ). In addition, the hepatotoxicity of DTN in enzyme induction/inhibition mice was further investigated in the aspects of biochemistry, histopathology, and pharmacokinetics. Administration of DTN in combination with KTZ resulted in attenuated liver injury, including lower alanine transaminase and aspartate transaminase activities and greater AUC and Cmax of serum DTN, whereas, pretreatment with dexamethasone aggravated the injury. Collectively, our findings illustrated that DTN-induced hepatotoxicity correlated well with the expression of CYP3A4, namely inhibition of CYP3A4 alleviated the toxicity both in vitro and in vivo, and induction aggravated the toxicity effects.

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Safety issues and new rapid detection methods in traditional Chinese medicinal materials

Cited by 41 publications

References 69 publications

Internal transcribed spacer guided multiplex PCR for species identification of Convolvulus prostratus and Evolvulus alsinoides

Internal transcribed spacer guided multiplex PCR for species identification of Convolvulus prostratus and Evolvulus alsinoides

A holistic strategy for quality and safety control of traditional Chinese medicines by the “iVarious” standard system

The Modulatory Role of CYP3A4 in Dictamnine-Induced Hepatotoxicity

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