Fast acoustic tweezers for the two-dimensional manipulation of individual particles in microfluidic channels

Tran, Si Bui Quang; Marmottant, Philippe; Thibault, Pierre

doi:10.1063/1.4751348

Cited by 87 publications

(74 citation statements)

References 19 publications

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“…Sparked by the ambition to dynamically manipulate microparticles in solution, there have been major advances in the development of experimental methods to control ultrasound acoustic fields at the microscale [1,2]: for example, using bulk acoustic waves [3][4][5], surface acoustic waves [6][7][8][9], transducer arrays [10][11][12], and 3D-printed transmission holograms [13]. The acoustic radiation force acting on particles in acoustic fields is used in these systems to manipulate particles and cells, thereby concentrating [14], trapping [15,16], separating [17], and sorting [18] bioparticles and cells based on their acoustomechanical properties.…”

Section: Introductionmentioning

confidence: 99%

Acoustic Tweezing and Patterning of Concentration Fields in Microfluidics

Karlsen

Bruus

2017

Phys. Rev. Applied

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We demonstrate theoretically that acoustic forces acting on inhomogeneous fluids can be used to pattern and manipulate solute concentration fields into spatiotemporally controllable configurations stabilized against gravity. A theoretical framework describing the dynamics of concentration fields that weakly perturb the fluid density and speed of sound is presented and applied to study manipulation of concentration fields in rectangular-channel acoustic eigenmodes and in Bessel-function acoustic vortices. In the first example, methods to obtain horizontal and vertical multilayer stratification of the concentration field at the end of a flow-through channel are presented. In the second example, we demonstrate acoustic tweezing and spatiotemporal manipulation of a local high-concentration region in a lower-concentration medium, thereby extending the realm of acoustic tweezing to include concentration fields.

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Section: Introductionmentioning

confidence: 99%

Acoustic Tweezing and Patterning of Concentration Fields in Microfluidics

Karlsen

Bruus

2017

Phys. Rev. Applied

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“…9 In addition, for a given power input, the acoustic radiation force can be up to five orders of magnitude greater than that that can be achieved with light for macroscopic objects. 10,11 Ultrasonic manipulation started with quasi one-dimensional (1D) 12 and two-dimensional (2D) 13 standing wave devices which trap Rayleigh particles (i.e., a ( k, where a is the particle radius and k is the wavelength) in regular patterns. For example, 4-transducer devices have been used to trap micro-particles in regular grids for applications such as tissue engineering.…”

mentioning

confidence: 99%

Three-dimensional ultrasonic trapping of micro-particles in water with a simple and compact two-element transducer

Franklin

Marzo

Malkin

et al. 2017

Applied Physics Letters

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We report a simple and compact piezoelectric transducer capable of stably trapping single and multiple micro-particles in water. A 3D-printed Fresnel lens is bonded to a two-element kerfless piezoceramic disk and actuated in a split-piston mode to produce an acoustic radiation force trap that is stable in three-dimensions. Polystyrene micro-particles in the Rayleigh regime (radius λ/14 to λ/7) are trapped at the focus of the lens (F# = 0.4) and manipulated in two-dimensions on an acoustically transparent membrane with a peak trap stiffness of 0.43 mN/m. Clusters of Rayleigh particles are also trapped and manipulated in three-dimensions, suspended in water against gravity. This transducer represents a significant simplification over previous acoustic devices used for micro-particle manipulation in liquids as it operates at relatively low frequency (688 kHz) and only requires a single electrical drive signal. This simplified device has potential for widespread use in applications such as micro-scale manufacturing and handling of cells or drug capsules in biomedical assays.

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“…A linear continuous phase modulation can be achieved by shifting a frequency in a small amount, because y = y 0 cos(kx − ωt −φt) = y 0 cos(kx − (ω +φ)t) [22]. Therefore, we altered the frequency of one channel of the function generator in our experiments to move pressure nodes along the direction perpendicular to the flow.…”

mentioning

confidence: 99%

Continuously phase-modulated standing surface acoustic waves for separation of particles and cells in microfluidic channels containing multiple pressure nodes

Lee¹,

Rhyou²,

Kang³

et al. 2017

J. Phys. D: Appl. Phys.

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This paper describes continuously phase-modulated standing surface acoustic waves (CPM-SSAW) and its application for particle separation in multiple pressure nodes. A linear change of phase in CPM-SSAW applies a force to particles whose magnitude depends on their size and contrast factors. During continuous phase modulation, we demonstrate that particles with the target dimension are translated in the direction of moving pressure nodes, whereas smaller particles show oscillatory movements. The rate of phase modulation is optimized for separation of target particles from the relationship between mean particle velocity and period of oscillation. The developed technique is applied to separate particles of the target dimension from the particle mixture. Furthermore, we also demonstrate human keratinocyte cells can be separated in the cell and bead mixture. The separation technique is incorporated with a microfluidic channel spanning multiple pressure nodes, which is advantageous over separation in a single pressure node in terms of high throughput.

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Fast acoustic tweezers for the two-dimensional manipulation of individual particles in microfluidic channels

Cited by 87 publications

References 19 publications

Acoustic Tweezing and Patterning of Concentration Fields in Microfluidics

Acoustic Tweezing and Patterning of Concentration Fields in Microfluidics

Three-dimensional ultrasonic trapping of micro-particles in water with a simple and compact two-element transducer

Continuously phase-modulated standing surface acoustic waves for separation of particles and cells in microfluidic channels containing multiple pressure nodes

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