We present a model applicable to ultrasound contrast agent bubbles that takes into account the physical properties of a lipid monolayer coating on a gas microbubble. Three parameters describe the properties of the shell: a buckling radius, the compressibility of the shell, and a break-up shell tension. The model presents an original non-linear behavior at large amplitude oscillations, termed compression-only, induced by the buckling of the lipid monolayer. This prediction is validated by experimental recordings with the high-speed camera Brandaris 128, operated at several millions of frames per second. The effect of aging, or the resultant of repeated acoustic pressure pulses on bubbles, is predicted by the model. It corrects a flaw in the shell elasticity term previously used in the dynamical equation for coated bubbles. The break-up is modeled by a critical shell tension above which gas is directly exposed to water.

The ability of collapsing (cavitating) bubbles to focus and concentrate energy, forces and stresses is at the root of phenomena such as cavitation damage, sonochemistry or sonoluminescence. In a biomedical context, ultrasound-driven microbubbles have been used to enhance contrast in ultrasonic images. The observation of bubble-enhanced sonoporation--acoustically induced rupture of membranes--has also opened up intriguing possibilities for the therapeutic application of sonoporation as an alternative to cell-wall permeation techniques such as electroporation and particle guns. However, these pioneering experiments have not been able to pinpoint the mechanism by which the violently collapsing bubble opens pores or larger holes in membranes. Here we present an experiment in which gentle (linear) bubble oscillations are sufficient to achieve rupture of lipid membranes. In this regime, the bubble dynamics and the ensuing sonoporation can be accurately controlled. The use of microbubbles as focusing agents makes acoustics on the micrometre scale (microacoustics) a viable tool, with possible applications in cell manipulation and cell-wall permeation as well as in microfluidic devices.

International audienceWe depict and analyse the successive steps of atomization of a liquid jet when a fast gas stream blows parallel to its surface. Experiments performed with various liquids in a fast air flow show that the liquid destabilization proceeds from a two-stage mechanism: a shear instability first forms waves on the liquid. The transient acceleration experienced by the liquid suggests that a Rayleigh–Taylor type of instability is triggered at the wave crests, producing liquid ligaments which further stretch in the air stream and break into droplets. The primary wavelength $\lambda\,{\sim}\,\delta (\rho_{1}/\rho_{2})^{1/2}$ is set by the vorticity thickness $\delta$, in the fast air stream and the liquid/gas density ratio $\rho_{1}/\rho_{2}$. The transverse corrugations of the crests have a size $\lperp\,{\sim}\,\delta {\We_{\delta}}^{-1/3} (\rho_{1}/\rho_{2})^{1/3}$, where $\We_{\delta}\,{=}\,\rho_{2}u_{2}^{2}\delta/\sigma$ is the Weber number constructed on the gas velocity $u_{2}$ and liquid surface tension $\sigma$. The ligament dynamics gives rise, after break-up, to a well-defined droplet size distribution whose mean is given by $\lperp$. This distribution bears an exponential tail characteristic of the broad size statistics in airblast sprays

Vascular plant mortality during drought has been strongly linked to a failure of the internal water transport system caused by the rapid invasion of air and subsequent blockage of xylem conduits. Quantification of this critical process is greatly complicated by the existence of high water tension in xylem cells making them prone to embolism during experimental manipulation. Here we describe a simple new optical method that can be used to record spatial and temporal patterns of embolism formation in the veins of water-stressed leaves for the first time. Applying this technique in four diverse angiosperm species we found very strong agreement between the dynamics of embolism formation during desiccation and decline of leaf hydraulic conductance. These data connect the failure of the leaf water transport network under drought stress to embolism formation in the leaf xylem, and suggest embolism occurs after stomatal closure under extreme water stress.

Cell aggregates are a tool for in vitro studies of morphogenesis, cancer invasion, and tissue engineering. They respond to mechanical forces as a complex rather than simple liquid. To change an aggregate's shape, cells have to overcome energy barriers. If cell shape fluctuations are active enough, the aggregate spontaneously relaxes stresses (“fluctuation-induced flow”). If not, changing the aggregate's shape requires a sufficiently large applied stress (“stress-induced flow”). To capture this distinction, we develop a mechanical model of aggregates based on their cellular structure. At stress lower than a characteristic stress τ*, the aggregate as a whole flows with an apparent viscosity η*, and at higher stress it is a shear-thinning fluid. An increasing cell–cell tension results in a higher η* (and thus a slower stress relaxation time t c ). Our constitutive equation fits experiments of aggregate shape relaxation after compression or decompression in which irreversibility can be measured; we find t c of the order of 5 h for F9 cell lines. Predictions also match numerical simulations of cell geometry and fluctuations. We discuss the deviations from liquid behavior, the possible overestimation of surface tension in parallel-plate compression measurements, and the role of measurement duration.

The intricate patterns of veins that adorn the leaves of land plants are among the most important networks in biology. Water flows in these leaf irrigation networks under tension and is vulnerable to embolismforming cavitations, which cut off water supply, ultimately causing leaf death. Understanding the ways in which plants structure their vein supply network to protect against embolism-induced failure has enormous ecological and evolutionary implications, but until now there has been no way of observing dynamic failure in natural leaf networks. Here we use a new optical method that allows the initiation and spread of embolism bubbles in the leaf network to be visualized. Examining embolism-induced failure of architecturally diverse leaf networks, we found that conservative rules described the progression of hydraulic failure within veins. The most fundamental rule was that within an individual venation network, susceptibility to embolism always increased proportionally with the size of veins, and initial nucleation always occurred in the largest vein. Beyond this general framework, considerable diversity in the pattern of network failure was found between species, related to differences in vein network topology. The highest-risk network was found in a fern species, where single events caused massive disruption to leaf water supply, whereas safer networks in angiosperm leaves contained veins with composite properties, allowing a staged failure of water supply. These results reveal how the size structure of leaf venation is a critical determinant of the spread of embolism damage to leaves during drought.ne of the most striking features of leaves is the network of veins that function as microfluidic circuits responsible for importing water and nutrients and exporting sugars. These microfluidic circuits supply water to tissues engaged in photosynthesis (1), thus governing the rate of water and CO 2 exchange between plants and the atmosphere (2, 3). Despite the essential function of the leaf vasculature, its integrity is constantly under threat of failure because of the uncontrolled air embolization of the network when water stress exceeds a critical threshold value (4). Hence, leaf networks have evolved under competing selective pressures to simultaneously maximize efficiency (in terms of flow and construction cost) and safety (from embolism disruption) of water transport within the leaf (5). Major evolutionary transitions have affected the transport efficiency of the leaf vein network (6, 7), but little is known about adaptation of leaf networks to avoid catastrophic failure by air embolism.High water tension in the water-transporting xylem cells of plants originates in drying soils and, according to the "air-seeding" hypothesis, is responsible for pulling air into the continuous xylem water column through submicron "pit" pores in the thin, porous membranes that separate neighboring xylem conduits (8). Typically, the closure of stomatal valves on the leaf surface dramatically slows the pace of plant dehydration b...

The formation of bubbles by flow focusing of a gas and a liquid in a rectangular channel is shown to depend strongly on the channel aspect ratio. Bubble breakup consists in a slow linear 2D collapse of the gas thread, ending in a fast 3D pinch-off. The 2D collapse is predicted to be stable against perturbations of the gas-liquid interface, whereas the 3D pinch-off is unstable, causing bubble polydispersity. During 3D pinch-off, a scaling w_(m) approximately tau(1/3) between the neck width w_(m) and the time tau before breakup indicates that breakup is driven by the inertia of both gas and liquid, not by capillarity.

Carnivorous aquatic Utricularia species catch small prey animals using millimetre-sized underwater suction traps, which have fascinated scientists since Darwin's early work on carnivorous plants. Suction takes place after mechanical triggering and is owing to a release of stored elastic energy in the trap body accompanied by a very fast opening and closing of a trapdoor, which otherwise closes the trap entrance watertight. The exceptional trapping speed-far above human visual perception-impeded profound investigations until now. Using high-speed video imaging and special microscopy techniques, we obtained fully time-resolved recordings of the door movement. We found that this unique trapping mechanism conducts suction in less than a millisecond and therefore ranks among the fastest plant movements known. Fluid acceleration reaches very high values, leaving little chance for prey animals to escape. We discovered that the door deformation is morphologically predetermined, and actually performs a buckling/unbuckling process, including a complete trapdoor curvature inversion. This process, which we predict using dynamical simulations and simple theoretical models, is highly reproducible: the traps are autonomously repetitive as they fire spontaneously after 5 -20 h and reset actively to their ready-to-catch condition.