Continuously phase-modulated standing surface acoustic waves for separation of particles and cells in microfluidic channels containing multiple pressure nodes

Lee, Junseok; Rhyou, Chanryeol; Kang, Byungjun; Lee, Hyungsuk

doi:10.1088/1361-6463/aa62d5

Cited by 23 publications

(14 citation statements)

References 23 publications

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“…12d). When SSAW is combined with other techniques such as the frequency modulation using chirped IDTs 52 or the phase modulation 21 , it can be possible to arrange different types of microparticles or cells at distinct locations (Supplementary Fig. 13).…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have shown that pressure fields formed by standing surface acoustic waves (SSAWs) are capable of manipulating microparticles at a high resolution in a noninvasive manner 21–26 . SSAW techniques also exhibit the potential to selectively manipulate various types of microparticles 21 , regulate cell–cell distances 22 , and engineer cellular aggregates such as spheroids 25 . Such high-resolution cell engineering is essential to replicate complex and highly ordered tissues in vivo because obtaining such tissues by current methods, including bioprinting, is difficult.…”

Section: Introductionmentioning

confidence: 99%

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High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy

et al. 2018

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The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery. Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function. Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels. Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors. The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue. Our study can be referenced to fabricate various types of artificial tissues that mimic the original functions as well as structures.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy

et al. 2018

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“…In conclusion, we presented an easily reconfigurable method for particle separation in surface wave microfluidic devices increasing the performance of acoustic sorting techniques that are based on the frequency difference between two transducers. 9 Bidirectional sorting has been demonstrated by adjusting the inflow rates and electrical signal of transducers. The separation distance achieved with this method is half the wavelength, which is double that of conventional time-of-flight methods.…”

Section: -3mentioning

confidence: 99%

“…8 Recently, a continuously frequency modulated acoustic field method was applied in a surface acoustic wave (SAW) device. 9 However, in this technique, the particle movement is fluctuating, demonstrating limited selectivity (2.5-fold or greater diameter ratio) and efficiency (up to 83%). We propose a modified method that increases both selectivity and efficiency to open up a wide range of applications in biomedical engineering and beyond.…”

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confidence: 99%

Particle separation in surface acoustic wave microfluidic devices using reprogrammable, pseudo-standing waves

Simon

Pailhas

Andrade

et al. 2018

Applied Physics Letters

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We report size and density/compressibility-based particle sorting using on-off quasi-standing waves based on the frequency difference between two ultrasonic transducers. The 13.3 MHz fundamental operating frequency of the surface acoustic wave microfluidic device allows the manipulation of particles on the micrometer scale. Experiments, validated by computational fluid dynamics, were carried out to demonstrate size-based sorting of 5–14.5 μm diameter polystyrene (PS) particles and density/compressibility-based sorting of 10 μm PS, iron-oxide, and poly(methyl methacrylate) particles, with densities ranging from 1.05 to 1.5 g/cm3. The method shows a sorting efficiency of >90% and a purity of >80% for particle separation of 10 μm and 14.5 μm, demonstrating better performance than similar sorting methods recently published (72%–83% efficiency). The sorting technique demonstrates high selectivity separation of particles, with the smallest particle ratio being 1.33, compared to 2.5 in previous work. Density/compressibility-based sorting of polystyrene and iron-oxide particles showed an efficiency of 97 ± 4% and a purity of 91 ± 5%. By varying the sign of the acoustic excitation signal, continuous batch acoustic sorting of target particles to a desired outlet was demonstrated with good sorting stability against variations of the inflow rates.

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“…The rapid separation and purification of biological particles have attracted considerable research attention recently due to their value in research, especially in medical diagnosis and environmental detection applications [ 1 , 2 , 3 ]. Centrifugation [ 4 , 5 ] is the most frequently used technology for separating biological particles.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoresis Separation of Platelets Using a Novel Zigzag Microchannel

et al. 2020

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Platelet separation and purification are required in many applications including in the detection and treatment of hemorrhagic and thrombotic diseases, in addition to transfusions and in medical research. In this study, platelet separation was evaluated using a novel zigzag microchannel fluidic device while leveraging a dielectrophoresis (DEP) electric field using the COMSOL multiphysics software package and additional experimentation. The zigzag-shaped microchannel was superior to straight channel devices for cell separation because the sharp corners reduced the required horizontal distance needed for separation and also contributed to an asymmetric DEP electric field. A perfect linear relationship was observed between the separation distance and the corner angles. A quadratic relationship (R2 = 0.99) was observed between the driving voltage and the width and the lengths of the channel, allowing for optimization of these properties. In addition, the voltage was inversely proportional to the channel width and proportional to the channel length. An optimal velocity ratio of 1:4 was identified for the velocities of the two device inlets. The proposed device was fabricated using laser engraving and lithography with optimized structures including a 0.5 mm channel width, a 120° corner angle, a 0.3 mm channel depth, and a 17 mm channel length. A separation efficiency of 99.4% was achieved using a voltage of 20 V and a velocity ratio of 1:4. The easy fabrication, lower required voltage, label-free detection, high efficiency, and environmental friendliness of this device make it suitable for point-of-care medicine and biological applications. Moreover, it can be used for the separation of other types of compounds including lipids.

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Continuously phase-modulated standing surface acoustic waves for separation of particles and cells in microfluidic channels containing multiple pressure nodes

Cited by 23 publications

References 23 publications

High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy

High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy

Particle separation in surface acoustic wave microfluidic devices using reprogrammable, pseudo-standing waves

Dielectrophoresis Separation of Platelets Using a Novel Zigzag Microchannel

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