Factors affecting Zantedeschia Spreng. dedifferentiation in vitro

Abstract

Zantedeschia Spreng. is an economically important ornamental plant species. Improvement of aesthetic parameters and creation of novel variations of decorative plants are important economic goals for the commercial ornamental industry. The effect of the combination of growth regulators and the explant type on dedifferentiation induction in Zantedeschia aethiopica and Zantedeschia elliottianna was investigated. Research was carried out at the Institute of Biology and Plant Biotechnology of Aleksandras Stulginskis University and at the Laboratory of Agrobiotechnology of the Joint Research Centre in 2016-2017. Explants (leaf discs, spathe discs, and petiole segments) were cultured on the MS medium supplemented with different concentrations of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 6-benzylaminopurine (BAP), 30.0 g l -1 sucrose, and 8.0 g l -1 agar. Leaf discs and spathe discs did not show any response in the medium without growth regulators, while 16.3% (Zantedeschia aethiopica) and 24.3% (Zantedeschia elliottianna) of isolated petiole segments formed callus. Our results showed that an appropriate combination of growth regulators for callus induction varied depending on the genetic background and the explant type. It was documented that isolated petiole segments of the arum lily and the calla lily induced more statistically reliable callus in a medium supplemented with combination BAP + IAA, while the combination BAP + IBA promoted callus formation from spathe disc tissues. Petiole segments manifested the highest dedifferentiation capability among the tested explant types. The results of the study showed that the ability of the somatic tissues of the arum lily and the calla lily to induce dedifferentiation seems to be a valuable material for improvement of ornamental values in these plants.

Coping with the cold: unveiling cryoprotectants, molecular signaling pathways, and strategies for cold stress resilience

“…The application of exogenous cryoprotectants containing proline significantly elevates the endogenous proline levels and enhances cold tolerance. Studies have shown that prolinemediated cryoprotectant treatments in rapeseed shoots increase cold tolerance and reduce electrolyte leakage, which is an indicator of membrane injury (Jonytiene et al, 2012). Additionally, prolinetreated Brassica juncea has been shown to protect isolated thylakoid membranes from photoinhibition (Bhandari and Nayyar, 2014) and improve the maintenance of chloroplast ultrastructure and membrane integrity in tobacco plants (Van Rensburg et al, 1993).…”

Calla lily soft rot causal agents, symptoms, virulence and management: a review

“…Calla lilies in the Zantedeschia section have white flowers while those in the Aestivae section have a variety of colours. Consequently, there are several varieties of calla lily used as cut flowers and pot plants depending on the flower shape and colours (Jonytienė et al, 2017) and the vase life (Lazzereschi et al, 2011). Commercially, calla lily is one of the major cut flowers traded on the international market.…”

Establishment of an efficient regeneration and Agrobacterium transformation system in mature embryos of calla lily (Zantedeschia spp.)

“…It has been reported that Z. aethiopica and Z. hybrida (interspecies hybrids mainly generated from Z. elliotiana , Z. pentlandii , Z. albinoscapulata , and Z. rehmannii , etc. ) have been utilized for induction, and variation in induction rates of Z. aethiopica was 16.3% while Z. hybrida was 24.4% ( Jonytienė et al, 2017 ). In conclusion, the combination and concentration of PGRs have been thoroughly examined to evaluate their effects on several plant tissue culture regeneration processes, such as callus formation and root induction.…”