Factors affecting the growth of bifidobacteria in human milk

Ročková, Šárka; Nevoral, Jiří; Rada, V.; Maršík, Petr; Sklenář, Jan; Hinkova, Andrea; Vlková, Eva; Marounek, M.

doi:10.1016/j.idairyj.2011.02.005

Cited by 16 publications

(11 citation statements)

References 26 publications

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“…In our previous study, the growth of different species of bifidobacteria in human milk was tested and significant differences were discovered (Rockova et al, 2011). The aim of the present work was to test the influence of different kinds of animal milk on the growth of bifidobacteria.…”

mentioning

confidence: 96%

Growth of bifidobacteria in mammalian milk

Ročková¹,

Rada²,

Havlík³

et al. 2013

CZECH J ANIM SCI

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Microbial colonization of the mammalian intestine begins at birth, when from a sterile state a newborn infant is exposed to an external environment rich in various bacterial species. An important group of intestinal bacteria comprises bifidobacteria. Bifidobacteria represent major intestinal microbiota during the breast-feeding period. Animal milk contains all crucial nutrients for babies' intestinal microflora. The aim of our work was to test the influence of different mammalian milk on the growth of bifidobacteria. The growth of seven strains of bifidobacteria in human milk, the colostrum of swine, cow's milk, sheep's milk, and rabbit's milk was tested. Good growth accompanied by the production of lactic acid was observed not only in human milk, but also in the other kinds of milk in all three strains of Bifidobacterium bifidum of different origin. Human milk selectively supported the production of lactic acid of human bifidobacterial isolates, especially the Bifidobacterium bifidum species. The promotion of bifidobacteria by milk is speciesspecific. Human milk contains a key factor for the growth of specific species or strains of human-origin bifidobacteria compared to other kinds of milk. In contrast, some components (maybe lysozyme) of human milk inhibited the growth of Bifidobacterium animalis. Animal-origin strains of bifidobacteria were not able to significantly grow even in milk of animal origin, with the exception of B. animalis subsp. lactis 1,2, which slightly grew in sheep's milk.

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mentioning

confidence: 96%

Growth of bifidobacteria in mammalian milk

Ročková¹,

Rada²,

Havlík³

et al. 2013

CZECH J ANIM SCI

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“…HMOs were isolated and purified from breast milk samples obtained from volunteer mothers as described by Rockova et al [11]. Stock solution of HMOs (c = 23.5 g/L) were prepared using phosphate-buffered saline (PBS) and syringe microfilters (pore size 0.2 μm; Whatman ® Anotop ® IC and LC; Whatman GmbH, Germany) and used immediately after preparation.…”

Section: Hmos Preparationmentioning

confidence: 99%

Influence of human milk oligosaccharides on adherence of bifidobacteria and clostridia to cell lines

Musilová

Modrackova

Doskočil

et al. 2017

AMicr

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Adhesion of gut bacteria to the intestinal epithelium is the first step in their colonization of the neonatal immature gut. Bacterial colonization of the infant gut is influenced by several factors, of which the most important are the mode of delivery and breast-feeding. Breast-fed infants ingest several grams of human milk oligosaccharides (HMOs) per day, which can become receptor decoys for intestinal bacteria. The most abundant intestinal bacteria in vaginally delivered infants are bifidobacteria, whereas infants born by cesarean section are colonized by clostridia. The influence of HMOs on the adhesion of five strains of intestinal bacteria (three bifidobacterial strains and two clostridial strains) to mucus-secreting and non-mucus-secreting human epithelial cells was investigated. Bifidobacterium bifidum 1 and Bifidobacterium longum displayed almost the same level of adhesion in the presence and absence of HMOs. By contrast, adhesion of Clostridium butyricum 1 and 2 decreased from 14.41% to 6.72% and from 41.54% to 30.91%, respectively, in the presence of HMOs. The results of this study indicate that HMOs affect bacterial adhesion and are an important factor influencing bacterial colonization of the gut. Adhesion of the tested bacteria correlates with their ability to autoaggregate.

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“…An infant-associated bacterial commensal shown to use breast milk sialyloligosaccharides (Sela et al, 2011) Human milk oligosaccharides TOF/TOF (DHB) Oligosaccharides Study of factors affecting the growth of bifidobacteria in milk (Rockova et al, 2011) Human milk oligosaccharides FT-ICR Oligosaccharides…”

Section: Sialylated Glycansmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

Harvey

2015

Mass Spectrometry Reviews

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This review is the seventh update of the original article published in 1999 on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2012. General aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, and fragmentation are covered in the first part of the review and applications to various structural types constitute the remainder. The main groups of compound are oligo- and poly-saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Much of this material is presented in tabular form. Also discussed are medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 36:255-422, 2017.

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Factors affecting the growth of bifidobacteria in human milk

Cited by 16 publications

References 26 publications

Growth of bifidobacteria in mammalian milk

Growth of bifidobacteria in mammalian milk

Influence of human milk oligosaccharides on adherence of bifidobacteria and clostridia to cell lines

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

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