This study prepared a novel Phosphorene (PN) and load it onto TiO2 to fabricated PN-TiO2, to effectively photo degrade the hydrophobic environmental hormone 17ß-estradiol in aqueous solutions. First, the corresponding characterization of prepared materials was implemented by myriads of methods (e.g, atomic force microscopy), transmission electron microscopy, scanning electron microscopy, energy dispersive spectrometer, X-ray diffraction and Raman spectroscopy. Next, the effect of the PN on the degradation efficiency was systematically investigated. Apparently, the doping of TiO2 by PN significantly enhanced its photocatalytic and adsorption properties with the addition of PN, compared to that in the absence of TiO2. That is the addition improved the adsorption capability of the composite. The optimal PN weight content was found to be 0.5%. However, the photo degradation efficiency gradually decreased when the PN content increased further. This optimal PN content directly suggested synergistic interactions upon the photo degrading efficiency. Compare to other PN-based photocatalysts mentioned in literature, this PN-based material owned striking advantages, such as higher energy efficiency, greater remove capacity, and superior cost-effectiveness. Further, biotoxicity of the water after treatment decreased was evident by observing the development of zebrafish embryos. In conclusion, the prepared PN-based materials exhibited promising photocatalytic capabilities for removal and biotoxicity reduction of 17ß-estradiol in aqueous solutions.