Facile synthesis of 2D Europium-metal organic frameworks nanosheets for highly efficient electrochemiluminescence in DNA detection

Dai, Wenjie; Wang, Xiaoyan; Chen, Gaoxu; Wang, Xue; Hu, Congyi; Zhen, Shu Jun; Huang, Chengzhi; Li, Yuanfang

doi:10.1016/j.cej.2023.143037

Cited by 24 publications

(8 citation statements)

References 37 publications

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“…Furthermore, the X-ray photoelectron spectroscopy (XPS) of the Eu-MOGs (Figure A) presented obvious peaks of Eu 3d, C 1s, N 1s, and O 1s. In addition, the binding energies of typical Eu 3d 3/2 and 3d 5/2 peaks were located at 1165 and 1135 eV, respectively (Figure B), indicating that europium ions coordinated with H3-tctpy in trivalent form …”

Section: Resultsmentioning

confidence: 98%

Renewable Electrochemiluminescence Biosensor Based on Eu-MOGs as a Highly Efficient Emitter and a DNAzyme-Mediated Dual-drive DNA Walker as a Signal Amplifier for Ultrasensitive Detection of miRNA-222

Chen,

Guo,

Xiao

et al. 2024

Anal. Chem.

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Herein, novel europium metal−organic gels (Eu-MOGs) with excellent cathode electrochemiluminescence (ECL) emission are first used to construct biosensors for the ultrasensitive detection of miRNA-222. Impressively, N and O elements of organic ligand 2,2′:6,2″-terpyridine 4,4′,4″-tricarboxylic acid (H3-tctpy) can perfectly coordinate with Eu 3+ to form Eu-MOGs, which not only reduce nonradiative transition caused by the intramolecular free rotation of phenyl rings in other MOGs to enhance the ECL signal with extraordinary ECL efficiency as high as 37.2% (vs the [Ru(bpy) 3 ] 2+ /S 2 O 8 2− ECL system) but also reinforce ligand-to-metal charge transfer (LMCT) by the strong affinity between Eu 3+ and N and O elements to greatly improve the stability of ECL signals. Besides, an improved nucleic acid cascade amplification reaction is developed to greatly raise the conversion efficiency from target miRNA-222 to a DNAzyme-mediated dual-drive DNA walker as output DNA, which can simultaneously shear the specific recognition sites from two directions. In that way, the proposed biosensor can further enhance the detection sensitivity of miRNA-222 with a linear range of 10 aM−1 nM and a detection limit (LOD) of 8.5 aM, which can also achieve an accurate response in cancer cell lysates of MHCC-97L and HeLa. Additionally, the biosensor can be self-regenerated by the folding/unfolding of related triplets with pH changes to simplify experimental operations and reduce the cost. Hence, this work proposed novel MOGs with stable and intense ECL signals for the construction of a renewable ECL biosensor, supplying a reliable detection method in biomarker analysis and disease diagnosis.

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Section: Resultsmentioning

confidence: 98%

Renewable Electrochemiluminescence Biosensor Based on Eu-MOGs as a Highly Efficient Emitter and a DNAzyme-Mediated Dual-drive DNA Walker as a Signal Amplifier for Ultrasensitive Detection of miRNA-222

Chen,

Guo,

Xiao

et al. 2024

Anal. Chem.

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“…The metal–organic framework (MOF) is a type of crystalline material which is composed of central metal ions or clusters and bridged organic ligands. − Due to its excellent performance of designable and tunable function, the MOF material has strong application outlooks in the electrochemical biosensor domain, particularly in terms of the MOF as the ECL luminophore. − More impressively, some dual-ligand MOF materials with better performance have been developed that combine different functional ligands to achieve dual functionalization. For instance, Zhang’s group prepared the M6-MOF with two links of 1,3,6,8-tetra (4′-carboxyphenyl) pyrene (TABPy) and 5,10,15,20-tetra(4-carboxyphenyl)porphyrin (TCPP), where TCPP facilitated electron transfer to enhance the ECL emission of TABPy .…”

Section: Introductionmentioning

confidence: 99%

Ratiometric Electrochemiluminescence of Zirconium Metal–Organic Framework as a Single Luminophore for Sensitive Detection of HPV-16 DNA

Chen,

Dai,

et al. 2023

Anal. Chem.

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This study developed a new zirconium metal− organic framework (MOF) luminophore named Zr-DPA@TCPP with dual-emission electrochemiluminescence (ECL) characteristics at a resolved potential. First, Zr-DPA@TCPP with a core− shell structure was effectively synthesized through the self-assembly of 9,10-di(p-carboxyphenyl)anthracene (DPA) and 5,10,15,20tetra(4-carboxyphenyl)porphyrin (TCPP) as the respective organic ligands and the Zr cluster as the metal node. The reasonable integration of the two organic ligands DPA and TCPP with ECL properties into a single monomer, Zr-DPA@TCPP, successfully exhibited synchronous anodic and cathodic ECL signals. Besides, due to the impressively unique property of ferrocene (Fc), which can quench the anodic ECL but cannot affect the cathodic ECL signal, the ratiometric ECL biosensor was cleverly designed by using the cathode signal as an internal reference. Thus, combined with DNA recycle amplification reactions, the ECL biosensor realized sensitive ratiometric detection of HPV-16 DNA with the linear range of 1 fM−100 pM and the limit of detection (LOD) of 596 aM. The distinctive dual-emission properties of Zr-DPA@TCPP provided a new idea for the development of ECL luminophores and opened up an innovative avenue of fabricating the ratiometric ECL platform.

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“…MOF is another kind of porous material, which is formed by metal ions and organic ligands via coordination effects. 2D MOF can be prepared by choosing suitable experimental conditions [33,34]. For example, Dai et al prepared a 2D Eu-MOF with high crystalline degree and rigid skeletons, and the MOF could facilitate electron transfer [33].…”

Section: Introductionmentioning

confidence: 99%

“…2D MOF can be prepared by choosing suitable experimental conditions [33,34]. For example, Dai et al prepared a 2D Eu-MOF with high crystalline degree and rigid skeletons, and the MOF could facilitate electron transfer [33]. However, it is still necessary to improve the stability and the conductivity of 2D COF or MOF meeting the demands of new applications.…”

Section: Introductionmentioning

confidence: 99%

Porous carbon nanosheets derived from two-dimensional Fe-MOF for simultaneous voltammetric sensing of dopamine and uric acid

Chen,

Zhou,

et al. 2023

Nanotechnology

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It is of great significance for electrochemical sensors to simultaneously detect dopamine (DA) and uric acid (UA) related to biological metabolism. In this work, two-dimensional (2D) porous carbon nanosheets (CNS) was prepared as electrocatalysts to improve the sensitivity, the selectivity, and the detection limit of the simultaneous detection. First, 2D amorphous iron-metal organic frameworks (Fe-MOF) was synthesized with Fe3+ and terephthalic acid via a facile wet chemistry method at room temperature. And then, CNS was prepared by pyrolysis and pickling of Fe-MOF. CNS had large specific surface area, good electrical conductivity and lots of carbon defects. The response currents of the CNS modified electrode was larger than those of the control electrodes in the simultaneous determination. The simultaneous determination were measured via difference pulse voltammetry to reduce the effect of capacitive currents on quantitative analysis. The CNS modified electrodes showed high sensitivity and low detection limit for the simultaneous detection of DA and UA. The modified electrodes have been successfully used to detect DA and UA in normal human serum.

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Facile synthesis of 2D Europium-metal organic frameworks nanosheets for highly efficient electrochemiluminescence in DNA detection

Cited by 24 publications

References 37 publications

Renewable Electrochemiluminescence Biosensor Based on Eu-MOGs as a Highly Efficient Emitter and a DNAzyme-Mediated Dual-drive DNA Walker as a Signal Amplifier for Ultrasensitive Detection of miRNA-222

Renewable Electrochemiluminescence Biosensor Based on Eu-MOGs as a Highly Efficient Emitter and a DNAzyme-Mediated Dual-drive DNA Walker as a Signal Amplifier for Ultrasensitive Detection of miRNA-222

Ratiometric Electrochemiluminescence of Zirconium Metal–Organic Framework as a Single Luminophore for Sensitive Detection of HPV-16 DNA

Porous carbon nanosheets derived from two-dimensional Fe-MOF for simultaneous voltammetric sensing of dopamine and uric acid

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