Ratiometric Electrochemiluminescence of  Zirconium Metal–Organic Framework as a Single Luminophore for Sensitive Detection of HPV-16 DNA

Chen, Gaoxu; Dai, Wenjie; Hu, Congyi; Zang, Hao; Sun, Shiyi; Zhen, Shujun; Zhan, Lei; Huang, Chengzhi; Li, Yuanfang

doi:10.1021/acs.analchem.3c04710

Cited by 8 publications

(3 citation statements)

References 32 publications

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“…For Ni/Co−MOF−TCPP−Fc, the absorption peak of TCPP at 1270 cm −1 was weakened, indicating that the hydrogen on −OH was replaced by metal ions and formed an Me−O bond. The reduction in peak intensity at 1600 and 1690 cm −1 was possibly attributed to alterations in the electronic structure that occurred when porphyrin was incorporated as a ligand within the MOF [ 37 , 38 ]. A broad absorption band was observed for the pure substance of Fc−COOH between 3310~2500 cm −1 due to the O−H stretching vibration of the carboxyl group in Fc−COOH, and a stretching vibration peak of the C=C in cyclopentadiene was observed at 1475 cm −1 [ 39 , 40 ].…”

Section: Resultsmentioning

confidence: 99%

Construction of Metal–Organic Framework as a Novel Platform for Ratiometric Determination of Cyanide

Sun,

Wu,

Zong

et al. 2024

Biosensors

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Metal–organic frameworks (MOFs) are frequently utilized as sensing materials. Unfortunately, the low conductivity of MOFs hinder their further application in electrochemical determination. To overcome this limitation, a novel modification strategy for MOFs was proposed, establishing an electrochemical determination method for cyanides in Baijiu. Co and Ni were synergistically used as the metal active centers, with meso−Tetra(4−carboxyphenyl)porphine (TCPP) and Ferrocenecarboxylic acid (Fc−COOH) serving as the main ligands, synthesizing Ni/Co−MOF−TCPP−Fc through a hydrothermal method. The prepared MOF exhibited improved conductivity and stable ratio signals, enabling rapid and sensitive determination of cyanides. The screen−printed carbon electrodes (SPCE) were suitable for in situ and real−time determination of cyanide by electrochemical sensors due to their portability, low cost, and ease of mass production. A logarithmic linear response in the range of 0.196~44 ng/mL was demonstrated by this method, and the limit of detection (LOD) was 0.052 ng/mL. Compared with other methods, the sensor was constructed by a one−step synthesis method, which greatly simplifies the analysis process, and the determination time required was only 4 min. During natural cyanide determinations, recommended readouts match well with GC−MS with less than 5.9% relative error. Moreover, this electrochemical sensor presented a promising method for assessing the safety of cyanides in Baijiu.

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Section: Resultsmentioning

confidence: 99%

Construction of Metal–Organic Framework as a Novel Platform for Ratiometric Determination of Cyanide

Sun,

Wu,

Zong

et al. 2024

Biosensors

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“…Lu et al reported a sandwich-type ECL immunosensor based on Zr 6 -5,10,15,20-tetrakis (4-carboxyphenyl) porphyrin framework (PCN-224) for the determination of cardiac troponin I . Li’s team proposed a new zirconium metal–organic framework (Zr-DPA@TCPP) as a single luminophore, which showed synchronous anodic and cathodic ECL signals . Chen et al exploited a cage-like porous complex as the microreactor to attenuate the aggregation-caused quenching effect of meso-tetra(4-carboxyphenyl) porphine .…”

Section: Introductionmentioning

confidence: 99%

Silver Nanoparticles In Situ Enhanced Electrochemiluminescence of the Porphyrin Organic Matrix for Highly Sensitive and Rapid Monitoring of Tetracycline Residues

Han,

Shi,

Kang

et al. 2024

J. Agric. Food Chem.

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Accurate monitoring of tetracycline (TC) residues in the environment is crucial for avoiding contaminant risk. Herein, a novel TC biosensor was facilely designed by integrating silver nanoparticles (Ag NPs) into the porphyrin metal−organic matrix (Ag@AgPOM) as a bifunctional electrochemiluminescence (ECL) probe. Different from the step-by-step synthesis of the coreaction accelerator and ECL emitter, the co-reaction accelerators Ag NPs were in situ-grown on the surface of 5,10,15,20-tetrakis (4-carboxyphenyl) porphyrin (TCPP) via a simple one-pot approach. Symbiotic Ag NPs on Ag@AgPOM formed an intimate interface and increased the collision efficiency of the ECL reaction, achieving the ECL enhancement of TCPP. Under the optimized conditions, the ternary ECL biosensor showed a wide linear detection range toward TC with a low detection limit of 0.14 fmol L −1 . Compared with the traditional HPLC and ELISA methods, satisfied analytical adaptability made this sensing strategy feasible to monitor TC in complex environmental samples.

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“…For instance, Lei’s team developed the m-MOF integrated with two ligands, with DPA as a luminophore and D-H2 as an intramolecular coreactant, which generated ECL without exogenous coreactants . Our group not only prepared a dual-ligand Zr-DPA@TCPP MOF, which displayed dual ECL emission at the cathode and anode, but also developed Zn-MOF with Hcptpy as the ligand which realized coreactant-free and dual-emission ECL . However, there are still disadvantages of low ECL efficiency.…”

Section: Introductionmentioning

confidence: 99%

Coreactant-Free Zirconium Metal–Organic Framework with Dual Emission for Ratiometric Electrochemiluminescence Detection of HIV DNA

Chen,

Hu,

Dai

et al. 2024

Anal. Chem.

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Owing to the limitations of dual-signal luminescent materials and coreactants, constructing a ratiometric electrochemiluminescence (ECL) biosensor based on a single luminophore is a huge challenge. This work developed an excellent zirconium metal−organic framework (MOF) Zr-TBAPY as a single ECL luminophore, which simultaneously exhibited cathodic and anodic ECL without any additional coreactants. First, Zr-TBAPY was successfully prepared by a solvothermal method with 1,3,6,8tetra(4-carboxyphenyl)pyrene (TBAPY) as the organic ligand and Zr 4+ cluster as the metal node. The exploration of ECL mechanisms confirmed that the cathodic ECL of Zr-TBAPY originated from the pathway of reactive oxygen species (ROS) as the cathodic coreactant, which is generated by dissolved oxygen (O 2 ), while the anodic ECL stemmed from the pathway of generated Zr-TBAPY radical itself as the anodic coreactant. Besides, N,Ndiethylethylenediamine (DEDA) was developed as a regulator to ECL signals, which quenched the cathodic ECL and enhanced the anodic ECL, and the specific mechanisms of its dual action were also investigated. DEDA can act as the anodic coreactant while consuming the cathodic coreactant ROS. Therefore, the coreactant-free ratiometric ECL biosensor was skillfully constructed by combining the regulatory role of DEDA with the signal amplification reaction of catalytic hairpin assembly (CHA). The ECL biosensor realized the ultrasensitive ratio detection of HIV DNA. The linear range was 1 fM to 100 pM, and the limit of detection (LOD) was as low as 550 aM. The outstanding characteristic of Zr-TBAPY provided new thoughts for the development of ECL materials and developed a new way of fabricating the coreactant-free and single-luminophore ratiometric ECL platform.

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Ratiometric Electrochemiluminescence of Zirconium Metal–Organic Framework as a Single Luminophore for Sensitive Detection of HPV-16 DNA

Cited by 8 publications

References 32 publications

Construction of Metal–Organic Framework as a Novel Platform for Ratiometric Determination of Cyanide

Construction of Metal–Organic Framework as a Novel Platform for Ratiometric Determination of Cyanide

Silver Nanoparticles In Situ Enhanced Electrochemiluminescence of the Porphyrin Organic Matrix for Highly Sensitive and Rapid Monitoring of Tetracycline Residues

Coreactant-Free Zirconium Metal–Organic Framework with Dual Emission for Ratiometric Electrochemiluminescence Detection of HIV DNA

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