Facile single-step bioconjugation of the antifungal agent caspofungin onto material surfaces via an epoxide plasma polymer interlayer

Michl, Thomas D.; Giles, Carla; Cross, Alasdair T.; Griesser, Hans J.; Coad, Bryan R.

doi:10.1039/c7ra03897f

Cited by 16 publications

(23 citation statements)

References 20 publications

(4 reference statements)

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“…A promissory polymer for antifungal coatings made of allyl glycidyl ether polymerized by plasma treatment was functionalized via the spontaneous reaction between amine and epoxide groups on the surface, as can be seen in Figure 11. Caspofungingrafted polymers were very efficient against the two fungal pathogens C. albicans and C. glabrata; additionally, the materials showed biocompatibility (Michl, Giles, Cross, et al, 2017). More recently, caspofungin has been grafted onto polymethacrylates (Alex et al, 2020) such as poly(2-hydroxyethylmethacrylate) (PHEMA) (Michl, Giles, Mocny, et al, 2017) for antifungal activity against Candida and Aspergillus species.…”

Section: Addition Of Organic-fungicide Compounds To Polymersmentioning

confidence: 99%

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Antifungal polymers for medical applications

Velazco‐Medel¹,

Camacho‐Cruz²,

Lugo-González³

et al. 2020

Med Devices & Sens

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The application of polymers as medical devices has steadily increased in almost all medical fields because of the versatility of these materials. Thus, research has focused both on the development of more appropriate materials for specific situations and on the modification of already useful materials for the improvement of their intrinsic properties. Modifications on this kind of materials have increased their potential uses by adapting their mechanical properties to specific needs. Moreover, biocompatibility of the polymeric materials has been improved by the inclusion of certain functional groups, providing responses to physical and chemical stimuli present in physiological conditions.Until recently, one of the most worrying problems in hospitals has been infections derived from medical devices usage. Typically, this kind of infections was handled with the use of prophylactic and therapeutic treatments with 'classic' (low-molecular weight) antimicrobial agents. This strategy has been effective in most patients suffering from nosocomial infections. However, it has the disadvantage of substantially increasing the probability of antimicrobial-resistant pathogens appearance, which continue to be especially dangerous in hospital environments (Cohen et al., 2017; World Health Organization, n.d.;Zegers et al., 2017). Additionally, due to

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Section: Addition Of Organic-fungicide Compounds To Polymersmentioning

confidence: 99%

“…Covalent immobilization of caspofungin by amine nucleophilic attack to epoxy groups onto polymeric surface (Michl, Giles, Cross, et al, 2017) molecules inside attached by hydrogen bond and other non-covalent interactions. The chemical nature of the pendant groups allows micelle formation, as represented in Figure 12.…”

Section: Drug Delivery Sys Temsmentioning

confidence: 99%

Antifungal polymers for medical applications

Velazco‐Medel¹,

Camacho‐Cruz²,

Lugo-González³

et al. 2020

Med Devices & Sens

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“…Coad et al demonstrated irreversible attachment of the antifungal drug caspofungin using a substrate-independent coating technique which could be applicable to different bulk biomaterials as an essentially two-dimensional surface coating (Coad et al, 2015;Griesser et al, 2015;Michl et al, 2017a). It was shown that using only water or buffer washes was not sufficient in removing the reversibly-bound caspofungin.…”

Section: Grafted Antifungalsmentioning

confidence: 99%

“…The surface coating was also found to have no adverse effects on attachment and growth of mammalian primary fibroblasts (Griesser et al, 2015). Finally, the killing ability of surfaces with attached caspofungin was not diminished even after pre-conditioning the surface with adsorbed layers of protein (Michl et al, 2017a). Kucharikova et al examined caspofungin binding to titanium surfaces (Kucharíková et al, 2015).…”

Section: Grafted Antifungalsmentioning

confidence: 99%

“…An alternative quantitative method that can be utilized to examine antifungal surfaces is the microtiter plate biofilm assay (Coenye and Nelis, 2010) where the detailed methodology has been described previously (Chandra et al, 2008;Michl et al, 2017a;Michl et al, 2017b). This assay provides a quantitative measurement of the colony forming units and the biofilm formation on antifungal and control surfaces.…”

Section: Assay Selectionmentioning

confidence: 99%

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The importance of fungal pathogens and antifungal coatings in medical device infections

Giles

Lamont-Friedrich

Michl

et al. 2018

Biotechnology Advances

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In recent years, increasing evidence has been collated on the contributions of fungal species, particularly Candida, to medical device infections. Fungal species can form biofilms by themselves or by participating in polymicrobial biofilms with bacteria. Thus, there is a clear need for effective preventative measures, such as thin coatings that can be applied onto medical devices to stop the attachment, proliferation, and formation of device-associated biofilms. However, fungi being eukaryotes, the challenge is greater than for bacterial infections because antifungal agents are often toxic towards eukaryotic host cells. Whilst there is extensive literature on antibacterial coatings, a far lesser body of literature exists on surfaces or coatings that prevent attachment and biofilm formation on medical devices by fungal pathogens. Here we review strategies for the design and fabrication of medical devices with antifungal surfaces. We also survey the microbiology literature on fundamental mechanisms by which fungi attach and spread on natural and synthetic surfaces. Research in this field requires close collaboration between biomaterials scientists, microbiologists and clinicians; we consider progress in the molecular understanding of fungal recognition of, and attachment to, suitable surfaces, and of ensuing metabolic changes, to be essential for designing rational approaches towards effective antifungal coatings, rather than empirical trial of coatings.

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Plasma polymerization for biomedical applications: A review

Coad

Favia

Vasilev

et al. 2022

Plasma Processes & Polymers

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Plasma polymers have long been of interest as thin film coatings on biomedical devices and products, to generate desirable surface properties for favorable biointerfacial interactions. Plasma polymers have also been used as platforms for the covalent immobilization of bioactive molecules. More recently, additional aspects have been investigated, such as selective prevention of adhesion of microbial pathogens, either via plasma polymers per se or including antimicrobial drugs. Plasma polymers have also been investigated for the release of silver ions and small organic molecules. Complementing lowpressure plasma approaches, processes at atmospheric pressure have attracted interest recently, including for nano/ biocomposite coatings. This contribution reviews the use of plasma polymers for intended biomedical applications, with a focus on more recent topic areas.

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Facile single-step bioconjugation of the antifungal agent caspofungin onto material surfaces via an epoxide plasma polymer interlayer

Cited by 16 publications

References 20 publications

Antifungal polymers for medical applications

Antifungal polymers for medical applications

The importance of fungal pathogens and antifungal coatings in medical device infections

Plasma polymerization for biomedical applications: A review

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