Facile nano‐free electrochemiluminescence biosensor for detection of sulphamethoxazole via tris(2,2′‐bipyridyl)ruthenium(II) and N ‐methyl pyrrolidone recognition

Abstract

The electrochemiluminescence (ECL) system based on the ruthenium complex has become a powerful tool in the field of analytical chemistry. However, the non-aqueous ECL luminescence system, which does not involve complex nanomodification, has not been widely used for the determination of analytes. In this study, N-methyl pyrrolidone was selected as the solvent, and it could also act as a co-reactant of Ru bpy 3 2 +. Based on this, a simple ECL system without nanomaterials was established. Strong ECL was generated. Furthermore, a quenching effect between the excited state of Ru bpy 3 2 + and sulphamethoxazole (SMZ) was observed. Based on this, a sensitive ECL sensor for detecting SMZ is constructed. A linear relationship between ECL signal quenching intensity (ΔI) and the logarithm of SMZ concentration (log C) in the concentration range of 1 × 10 −7-1 × 10 −5 mol/l is obtained. The limit of detection is as low as 3.33 × 10 −9 mol/l. The method has been applied to the detection of SMZ in tap water samples with different concentration levels with satisfactory results, and the recovery was 95.3-102.6%.