Electrochemiluminescence immunosensor based on ferrocene functionalized ZIF-8 quenching the electrochemiluminescence of Ru(bpy)32+-doped silica nanoparticles embodied N-butyl diethanolamine

Xu, Peng; Zhang, Yihe; Li, Xiaojian; Ren, Xiang; Fan, Dawei; Wang, Huan; Wei, Qin; Ju, Huangxian

doi:10.1016/j.snb.2020.129101

Cited by 15 publications

(5 citation statements)

References 45 publications

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“…The limit of detection (LOD) is 7 pg mL -1 . Table 1 demonstrates comparison of PCT detection performance using different methods ( Li et al, 2015 ; Ghrera, 2019 ; Molinero-Fernández et al, 2019 ; Gao et al, 2020 ; Xu et al, 2021a ; Xu et al, 2021b ; Yue et al, 2022 ). The LOD obtained on the fabricated sensor is lower than that obtained using ITO electrode modified with quantum dots (QD) labelled Ab ( Ghrera, 2019 ), or sandwich-type electrochemical immunoassay based on magnetic beads (MBs) ( Molinero-Fernández et al, 2019 ), or g-C 3 N 4 -NiCo 2 S 4 -carbon nanotubes-silver nanoparticles (g-C 3 N 4 -NiCo 2 S 4 -CNTs-AgNPs) sensor ( Xu et al, 2021b ).…”

Section: Resultsmentioning

confidence: 99%

A highly sensitive immunosensor based on nanochannel-confined nano-gold enhanced electrochemiluminescence for procalcitonin detection

Chang,

Gu,

et al. 2023

Front. Chem.

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Sensitive detection of procalcitonin (PCT) in serum is crucial for the timely diagnosis and treatment of rheumatoid arthritis. In this work, an electrochemiluminescence (ECL) detection platform is developed based on in-situ growth of Au nanoparticles (AuNPs) in nanochannels and an analyte-gated detection signal, which can realize ECL determination of PCT with high sensitivity. Vertically ordered mesoporous silica films with amine groups and uniform nanochannel array (NH2-VMSF) is easily grown on the supporting indium tin oxide (ITO) electrode through electrochemical assisted self-assembly method (EASA). Anchored by the amino groups, AuNPs were grown in-situ within the nanochannels to catalyze the generation of reactive oxygen species (ROS) and amplify the ECL signal of luminol. An immuno-recognitive interface is constructed on the outer surface of NH2-VMSF, through covalent immobilization of PCT antibodies. In the presence of PCT, the immunocomplex will hinder the diffusion of luminol and co-reactants, leading to a gating effect and decreased ECL signals. Based on this principle, the immunosensor can detect PCT in the range from 10 pg/mL to 100 ng mL-1 with a limit of detection (LOD) of 7 pg mL-1. The constructed immunosensor can also be used for detecting PCT in serum. The constructed sensor has advantages of simple fabrication and sensitive detection, demonstrating great potential in real sample analysis.

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Section: Resultsmentioning

confidence: 99%

A highly sensitive immunosensor based on nanochannel-confined nano-gold enhanced electrochemiluminescence for procalcitonin detection

Chang,

Gu,

et al. 2023

Front. Chem.

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“…Procalcitonin (PCT) secreted by thyroid C cells has shown a strong correlation with the severity of sepsis, which is recognized as a representative biomarker in the early diagnosis of sepsis. Xu et al [209] developed a quenching-typed ECL immunosensor based on ferrocene carboxylic acid (Fca) quenching the ECL of the Ru(bpy) 3 2+ and TPrA co-encapsulated ECL SiO 2 NPs. In this assay, good sensitivity for PCT detection was achieved by an electron-transfer mediated quenching mechanism which contributed to the low LOD of 0.85 pg/mL.…”

Section: Protein-based Non-cancer Biomarkersmentioning

confidence: 99%

Recent Advances in Electrochemiluminescence Emitters for Biosensing and Imaging of Protein Biomarkers

Yang

2023

Chemosensors

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Electrochemiluminescence (ECL) is a light-emitting process triggered by the high energy redox between electrochemically oxidized and reduced luminophores or some coreactive intermediate radicals, representing a blooming hot topic over decades with a wide variety of bioanalytical applications. Due to the superb sensitivity, ultralow background noise, specificity, ease of integration, and real-time and in situ analysis, ECL has been developed as a convenient and versatile technique for immunodiagnostics, nucleic acid analysis, and bioimaging. Discovering highly-efficient ECL emitters has been a promising subject that will benefit the development of sensitive bioanalytical methods with prominent potential prospects. To date, the interdisciplinary integrations of electrochemistry, spectroscopy, and nanoscience have brought up the continuous emergences of novel nanomaterials which can be flexibly conjugated with specific bio-recognition elements as functional ECL emitters for bioassays. Therefore, a critical overview of recent advances in developing highly-efficient ECL emitters for ultrasensitive detection of protein biomarkers is presented in this review, where six kinds of the most promising ECL nanomaterials for biosensing and imaging of various disease-related protein biomarkers are separately introduced with references to representative works. Finally, this review discusses the ongoing opportunities and challenges of ECL emitters in developing advanced bioassays for single-molecule analysis and spatiotemporally resolved imaging of protein biomarkers with future perspectives.

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“…25 tris(2,2′-Bipyridyl) ruthenium(ii) [Ru(bpy) 3 2+ ], as the traditional ECL reagent, is frequently used on ECL-based sensing platforms owing to the high ECL efficiency, chemical stability, and good biocompatibility. 26,27 However, Ru(bpy) 3…”

Section: ■ Introductionmentioning

confidence: 99%

“…Especially, ECL gained much attention in clinical diagnostics because it is not disturbed by scattered light and excitation light sources . tris(2,2′-Bipyridyl) ruthenium(ii) [Ru(bpy) 3 2+ ], as the traditional ECL reagent, is frequently used on ECL-based sensing platforms owing to the high ECL efficiency, chemical stability, and good biocompatibility. , However, Ru(bpy) 3 2+ is difficult to immobilize on electrodes due to its good water solubility, limiting the ECL property . Several nanocarriers have been explored to enhance the efficiency and stability of Ru(bpy) 3 2+ , such as mesoporous silica and poly(ethylenimine).…”

Section: Introductionmentioning

confidence: 99%

Dual-Mode Immunosensor for Electrochemiluminescence Resonance Energy Transfer and Electrochemical Detection of Rabies Virus Glycoprotein Based on Ru(bpy)₃²⁺-Loaded Dendritic Mesoporous Silica Nanoparticles

Wang

et al. 2022

Anal. Chem.

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Rabies is a serious zoonotic disease in almost all warm-blooded animals and causes fatal encephalitis. The detection of rabies virus (RABV) is critical and remains a significant challenge. Herein, an electrochemiluminescence resonance energy transfer (ECL-RET) and electrochemical (EC) dual-mode immunosensor was developed for highly sensitive detection of RABV glycoprotein. Dendritic mesoporous silica nanoparticles (DMSNs) were employed to load Ru(bpy)3 2+ and to obtain ECL probes (Ru@DMSNs). Ru@DMSNs were decorated on the electrode surface, followed by the modification of the RABV antibody (Ab1). RABV was specifically recognized and captured by Ab1, causing the decline of the ECL signal due to the obstruction of electron transfer. Additionally, manganese oxide nanoparticles (MnOx) modified with Ab2 can further quench the ECL signal of Ru@DMSNs via the RET between Ru@DMSNs and MnOx. Meanwhile, MnOx can catalyze the oxidation of o-phenylenediamine (o-PD), generating a significant differential pulse voltammetry (DPV) signal as a second signal to monitor RABV glycoprotein concentration. Consequently, an immunosensor was developed to achieve dual-signal detection of RABV and improve reliability. Under the optimal conditions, detection ranges of 0.10 pg·mL–1 to 10 ng·mL–1 for ECL (with an 88 fg·mL–1 detection limit) and 1 pg·mL–1 to 2 ng·mL–1 for EC (with a 0.1 pg·mL–1 detection limit) were obtained for RABV detection. The reliability of this immunoassay was validated by eight brain tissue samples. The results were found to be compatible with the results of the real-time reverse transcription-polymerase chain reaction (RT-PCR) assay, indicating the potential applicability of this method for RABV diagnosis.

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Electrochemiluminescence immunosensor based on ferrocene functionalized ZIF-8 quenching the electrochemiluminescence of Ru(bpy)32+-doped silica nanoparticles embodied N-butyl diethanolamine

Cited by 15 publications

References 45 publications

A highly sensitive immunosensor based on nanochannel-confined nano-gold enhanced electrochemiluminescence for procalcitonin detection

A highly sensitive immunosensor based on nanochannel-confined nano-gold enhanced electrochemiluminescence for procalcitonin detection

Recent Advances in Electrochemiluminescence Emitters for Biosensing and Imaging of Protein Biomarkers

Dual-Mode Immunosensor for Electrochemiluminescence Resonance Energy Transfer and Electrochemical Detection of Rabies Virus Glycoprotein Based on Ru(bpy)₃²⁺-Loaded Dendritic Mesoporous Silica Nanoparticles

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Electrochemiluminescence immunosensor based on ferrocene functionalized ZIF-8 quenching the electrochemiluminescence of Ru(bpy)32+-doped silica nanoparticles embodied N-butyl diethanolamine

Cited by 15 publications

References 45 publications

A highly sensitive immunosensor based on nanochannel-confined nano-gold enhanced electrochemiluminescence for procalcitonin detection

A highly sensitive immunosensor based on nanochannel-confined nano-gold enhanced electrochemiluminescence for procalcitonin detection

Recent Advances in Electrochemiluminescence Emitters for Biosensing and Imaging of Protein Biomarkers

Dual-Mode Immunosensor for Electrochemiluminescence Resonance Energy Transfer and Electrochemical Detection of Rabies Virus Glycoprotein Based on Ru(bpy)32+-Loaded Dendritic Mesoporous Silica Nanoparticles

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Dual-Mode Immunosensor for Electrochemiluminescence Resonance Energy Transfer and Electrochemical Detection of Rabies Virus Glycoprotein Based on Ru(bpy)₃²⁺-Loaded Dendritic Mesoporous Silica Nanoparticles