Facile green synthesis of gold nanoparticles from marine algae Gelidiella acerosa and evaluation of its biological Potential

Abstract

In this present research, the Gold nanoparticles (GA-AuNPs) produced from Gelidiella acerosa, marine algae using green synthesis method was evaluated for its antidiabetic, antibacterial and antioxidant activity. Well-developed GA-AuNPs were effectively characterized using advanced analytical instruments like HRTEM, UV-visible, SEM and XRD. This analysis exposed GA-AuNPs to be present with spherical, hexagonal structure and crystalline nature. The average size of GA-AuNPs particles was estimated to lie between 5.81 nm to 117.59 nm by using Debye-Scherer equation. The enzyme inhibitory property of (IC 50 ) of G. acerosa aqueous extract and synthesized gold nanoparticles from G. acerosa against α amylase and α-glucosidase enzyme was found to be 2.1 ± 0.01, 3.7 ± 0.01 µg/mL and 2.8 ± 0.02, 4.1 ± 0.01, respectively. Further antioxidant assessment of GA-AuNPs were examined using DPPH and FRAP assays, the results exhibited that the strong antioxidant activity were observed in all the concentrations when compared with standard. Finally antibacterial activity was evaluated the GA-AuNPs against gram positive and gram negative bacteria, the strong antibacterial activity was observed in both the bacterial strains when compared with standard antibiotic. The GA-AuNPs from marine algae was exhibited to be strong inhibitory activity against alpha amylase and alpha glucosidase enzyme, antioxidant activity and antibacterial activity, these GA-AuNPs are projected to have potential biological applications.

Synthesis, characterization, and biological screening of metal nanoparticles loaded gum acacia microgels

“…For instance, GNCo microgels exhibited 8.3% ± 0.32 α-amylase and 6.43% ± 0.18 α-glucosidase inhibition potential as compared to GA microgels which demonstrated negligible activity as shown in the Table 3. The moderate antidiabetic activity of GNAg microgels is due to the inherent ability of AgNPs to inhibit both α-amylase and α-glucosidase, thus support and augment the previously reported studies (Balan et al, 2016;Rajaram, Aiswarya, & Sureshkumar, 2015;Senthilkumar, Surendran, Sudhagar, & Ranjith Santhosh Kumar, 2019).…”

Bactericidal effect of gentamicin conjugated gold nanoparticles

“…AFM images again confirm the finding that GNPs 2 is more effective in bactericidal effect than GNPs 1 in conjugation with the GTM. The most probable mechanism for antibacterial activity of the GNPs could be due to the oxidative stress on the microbial cell leading to the formation of extracellular vacuole using the intracellular enzyme lactate dehydrogenase [22] and it thus facilitates GTM to internalise into the cell and kill bacteria by binding to ribosomal 16S ribosomal RNA in the 30S subunit of the ribosome, inhibiting protein synthesis [23]. These combined activities leading to the complete cell destruction could be seen in Fig.…”

Green Synthesis of Metal and Metal Oxide Nanoparticles: Principles of Green Chemistry and Raw Materials

“…Gold nanoparticles (AuNPs) are considered the most attractive among noble metal nanoparticles, because of their potential use in catalysis, optics, biomedicine, etc. The widespread application of AuNPs has aroused considerable interest for new AuNPs synthesis methods, avoiding hazardous chemicals [53,177,[193][194][195][196]. Alternative methods, including "green" synthetic approaches to produce AuNPs, are important for maintaining sustanable development.…”