Synthesis, characterization, and biological screening of metal nanoparticles loaded gum acacia microgels

Abstract

We report novel gum acacia (GA) based microgels composites for multifunctional biomedical application. High yield of spherical GA microgels particles within 5-50 μm size range was obtained via crosslinking the polymer in the reverse micelles of surfactantsodium bis (2-ethylhexyl) sulfosuccinate (NBSS) in gasoline medium. The prepared microgels were then utilized for in situ silver (Ag) and cobalt (Co) nanoparticles (NPs) synthesis to subsequently produce GNAg and GNCo nanocomposite microgels, respectively. Ag and Co NPs of particle of almost less than 40 nm sizes were homogenously distributed over the matrices of the prepared microgels, and therefore, negligible agglomeration effect was observed. Pristine GA microgels, and the nanocomposite microgels were thoroughly characterized through FTIR, DSC, TGA, XRD, SEM, EDS, and TEM. The well-characterized pristine GA microgels and the nanocomposite microgels were then subjected to multiple in vitro bioassays including antioxidant, antidiabetic, and antimicrobial activities as well as biocompatibility investigation. Our results demonstrate that the prepared nanocomposites in particular GNAg microgels exhibited excellent biomedical properties as compared to pristine GA microgels. Among the prepared samples, GNAg nanocomposites were highly active against Fusarium oxysporum and Aspergillus niger that show 47.73% ± 0.25 inhibition and 32.3% ± 2.0 with IC-50 of 220 μg ml −1 and 343 μg ml −1 , respectively. Moderate antidiabetic activity was also observed for GNAg nanocomposites with considerable inhibition of 15.34% ± 0.20 and 14.7% ± 0.44 for both α-glucosidase and α-amylase, respectively. Moreover, excellent antioxidant properties were found for both the GNAg and GNCo nanocomposites as compared to pristine GA microgels. A remarkable biocompatible nature of the nanocomposites in particular GNAg makes the novel GA composites, to be exploited for diverse biomedical applications.

Exploring the therapeutic potential of Hibiscus rosa sinensis synthesized cobalt oxide (Co3O4-NPs) and magnesium oxide nanoparticles (MgO-NPs)

“…The assay was carried out using well optimized previously reported methodology with couple of alterations ( Ihsan et al 2021 ). In the experiment, in an eppendorf tube an aliquot of 100 µL of the sample was mixed with 900 mL of TAC reagent (0.6 M sulphuric acid, 28 mM sodium phosphate and 4 mM ammonium molybdate, in 50 mL dH 2 0).…”

Phytochemistry and antibacterial efficacy of northeastern pakistani artemisia rutifolia stephan ex spreng. extracts against some clinical and phyto-pathogenic bacterial strains

“…The quantitative determination of total flavonoids content (TFC) was performed using the aluminum chloride colorimetric technique 16 with little modifications. Briefly, 20 μl test samples were taken from each stock solution, with the addition of 10 μl of aluminum chloride in 90 μl of water (w/v).…”

“…A calibration curve (R2= 0.98) was obtained by using gallic acid as a positive standard. The experiment was repeated thrice and results were noted, the expression of the result is mentioned with unit μg GAE/mg DW (as gallic acid equivalent milligram dry weight) 16 .…”

Complete life of cobalt nanoparticles loaded into cross-linked organic polymers: a review

“…This unique combination of metal nanoparticles and organic cross-linked polymeric network microgels has received great interest during the last few years because of its many applications in different fields such as medicine, 13 the purification of water, 14 and catalysis. 15 Hybrid microgels are mostly used for the catalytic generation of H 2 gas from NaBH 4 or NH 3 BH 3 and then used to produce hydrogen for the reduction of toxic chemicals such as nitroarenes 16 and organic dyes 17 from water.…”