Fabrication of Tyrosinase Biosensor Based on Multiwalled Carbon Nanotubes‐Chitosan Composite and Its Application to Rapid Determination of Coliforms

Yang, Cheng; Liu, Yajun; Huang, Jingjing; Xian, Yuezhong; Zhang, Zhonghai; Jin, Litong

doi:10.1002/elan.200704195

Cited by 12 publications

(3 citation statements)

References 40 publications

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“…In Figure 3 c,d, representative 5 μm × 5 μm morphology images of the chitosan film before and after the EPEG grafting are reported. The images confirmed the well-film forming properties of chitosan 71 and the successful process of deposition by inkjet printing. In fact, AFM revealed a continuous and homogeneous coating, suitable for the subsequent fabrication steps.…”

Section: Resultssupporting

confidence: 66%

Aqueous Processed Biopolymer Interfaces for Single-Cell Microarrays

Ferrara

Zito

Arrabito

et al. 2020

ACS Biomater. Sci. Eng.

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Single-cell microarrays are emerging tools to unravel intrinsic diversity within complex cell populations, opening up new approaches for the in-depth understanding of highly relevant diseases. However, most of the current methods for their fabrication are based on cumbersome patterning approaches, employing organic solvents and/or expensive materials. Here, we demonstrate an unprecedented green-chemistry strategy to produce single-cell capture biochips onto glass surfaces by all-aqueous inkjet printing. At first, a chitosan film is easily inkjet printed and immobilized onto hydroxyl-rich glass surfaces by electrostatic immobilization. In turn, poly(ethylene glycol) diglycidyl ether is grafted on the chitosan film to expose reactive epoxy groups and induce antifouling properties. Subsequently, microscale collagen spots are printed onto the above surface to define the attachment area for single adherent human cancer cells harvesting with high yield. The reported inkjet printing approach enables one to modulate the collagen area available for cell attachment in order to control the number of captured cells per spot, from single-cells up to double- and multiple-cell arrays. Proof-of-principle of the approach includes pharmacological treatment of single-cells by the model drug doxorubicin. The herein presented strategy for single-cell array fabrication can constitute a first step toward an innovative and environmentally friendly generation of aqueous-based inkjet-printed cellular devices.

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Section: Resultssupporting

confidence: 66%

Aqueous Processed Biopolymer Interfaces for Single-Cell Microarrays

Ferrara

Zito

Arrabito

et al. 2020

ACS Biomater. Sci. Eng.

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“…Because chitosan-CNTs can form a stable complex through noncovalent binding, the stability of CNTs in aqueous chitosan solution is greatly improved. As a result CNTs can be uniformly distributed in a chitosan film [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Selective Simultaneous Determination of Paracetamol and Uric Acid Using a Glassy Carbon Electrode Modified with Multiwalled Carbon Nanotube/Chitosan Composite

2010

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A multiwalled carbon nanotube/chitosan modified glassy carbon electrode (MWCNTs-CHT/GCE) has been used for simultaneous determination of paracetamol (PAR) and uric acid (UA). The measurements were carried out using differential pulse voltammetry (DPV), cyclic voltammetry (CV) and chronoamperometry (CA). DPV measurements showed a linear relationship between oxidation peak current and concentration of PAR and UA in phosphate buffer (pH 7) over the concentration range 2 mM to 250 mM, and 10 mM to 400 mM, respectively. The analytical performance of this sensor has been evaluated for detection of PAR and UA in human serum and human urine with satisfactory results.

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“…However, its poor electrical conductivity may limit its applicability. To overcome this issue, chitosan has generally been combined with nanomaterials, such as graphene [ 49 ], multi-walled carbon nanotubes [ 50 ], and metallic nanoparticles [ 20 , 34 , 36 ], as well as conducting polymers, such as polyaniline and polypyrrole [ 51 , 52 ]. Chitosan nanoparticles (ChitNPs) with a size smaller than 100 nm showed superior chemical and biological performances than pristine chitosan, making them an excellent source for biomedical and biotechnological applications [ 53 , 54 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Amperometric Biosensor Based on Tyrosinase/Chitosan Nanoparticles for Sensitive and Interference-Free Detection of Total Catecholamine

et al. 2022

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The regulation of nervous and cardiovascular systems and some brain-related behaviors, such as stress, panic, anxiety, and depression, are strictly dependent on the levels of the main catecholamines of clinical interest, dopamine (DA), epinephrine (EP), and norepinephrine (NEP). Therefore, there is an urgent need for a reliable sensing device able to accurately monitor them in biological fluids for early diagnosis of the diseases related to their abnormal levels. In this paper, we present the first tyrosinase (Tyr)-based biosensor based on chitosan nanoparticles (ChitNPs) for total catecholamine (CA) detection in human urine samples. ChitNPs were synthetized according to an ionic gelation process and successively characterized by SEM and EDX techniques. The screen-printed graphene electrode was prepared by a two-step drop-casting method of: (i) ChitNPS; and (ii) Tyr enzyme. Optimization of the electrochemical platform was performed in terms of the loading method of Tyr on ChitNPs (nanoprecipitation and layer-by-layer), enzyme concentration, and enzyme immobilization with and without 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as cross-linking agents. The Tyr/EDC-NHS/ChitNPs nanocomposite showed good conductivity and biocompatibility with Tyr enzyme, as evidenced by its high biocatalytic activity toward the oxidation of DA, EP, and NEP to the relative o-quinone derivatives electrochemically reduced at the modified electrode. The resulting Tyr/EDC-NHS/ChitNPs-based biosensor performs interference-free total catecholamine detection, expressed as a DA concentration, with a very low LOD of 0.17 μM, an excellent sensitivity of 0.583 μA μM−1 cm−2, good stability, and a fast response time (3 s). The performance of the biosensor was successively assessed in human urine samples, showing satisfactory results and, thus, demonstrating the feasibility of the proposed biosensor for analyzing total CA in physiological samples.

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Fabrication of Tyrosinase Biosensor Based on Multiwalled Carbon Nanotubes‐Chitosan Composite and Its Application to Rapid Determination of Coliforms

Cited by 12 publications

References 40 publications

Aqueous Processed Biopolymer Interfaces for Single-Cell Microarrays

Aqueous Processed Biopolymer Interfaces for Single-Cell Microarrays

Selective Simultaneous Determination of Paracetamol and Uric Acid Using a Glassy Carbon Electrode Modified with Multiwalled Carbon Nanotube/Chitosan Composite

Novel Amperometric Biosensor Based on Tyrosinase/Chitosan Nanoparticles for Sensitive and Interference-Free Detection of Total Catecholamine

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