Fabrication of Nano-Structured SPR Chip for Sensitivity Enhancement

Abstract

We used the Langmuir-Blodgett (LB) method for preparation of large area, defect-free monolayer silica particle film as a template for the fabrication of nano-structure Au pattern on a conventional Au substrate for Surface Plasmon Resonance (SPR). Well organized, trigonal pyramid shaped Au nano-structures were able to construct on 34 separate chips in one fabrication process. The dimensions of trigonal pyramid nano-structures were precisely controlled by changing the particle size of the silica LB template. The nano-structure Au patterned SPR chips provides the enhancement of sensitivity in SPR analysis. The sensitivity enhancement of nano-structure patterned chips was evaluated by comparison with conventional flat Au chips. The nano-structure patterned substrate demonstrated a sensitivity enhancement up to 120% compared to a conventional SPR chip when ethanol solution was used as an analyte.

Retroreflective Janus Microparticle as a Nonspectroscopic Optical Immunosensing Probe

“…Using the Langmuir–Blodgett (LB) film technique and the electron beam physical vapor deposition (EBPVD), 40 nm aluminum and 20 nm gold layers were sequentially constructed on the hemispherical surface of the silica particles (Scheme ). , The aluminum layer exhibiting high reflectance was chosen as a retroreflectivity enhancer and as an intermediate adhesive layer for the deposition of gold, which adheres poorly to the silica surface. The gold layer was employed to enhance the spatio-selective functionalization of the reacting antibodies using the self-assembled monolayer (SAM) technique.…”