Retroreflective Janus Microparticle as a Nonspectroscopic Optical Immunosensing Probe

Han, Yong Duk; Kim, Hyo-Sop; Park, Yoo Min; Chun, Hyeong Jin; Kim, Jae-Ho

doi:10.1021/acsami.6b02014

Cited by 45 publications

(42 citation statements)

References 35 publications

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“…According to the empirical model of retroreflection signaling, the incidence angle of white LED light to the focusing surface was 40°. 10 To achieve this angle, the white LED module should be installed adjacent to the objective lens of the microscope to face the sample stage. To acquire retroreflection signals, the microscope was set to the bright-field acquisition mode with the light source turned off.…”

Section: Methodsmentioning

confidence: 99%

Nonspectroscopic Migratory Cell Monitoring Method Using Retroreflective Janus Microparticles

et al. 2020

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This study aims to suggest a simple migratory cell monitoring method in the Transwell system by utilizing retroreflective Janus microparticles (RJPs) as an optical probe. The RJP could be internalized on cells without compromising the cell viability and can be registered as bright spots within the cell body by inducing retroreflection from nonspectroscopic light sources. Conventional optical probes (e.g., fluorophores, chromogens, and nanoparticles) have been extensively studied and applied across diverse platforms (e.g., Boyden chamber, wound closing, and microfluidic chips) for understanding in vitro kinetic cell behavior. However, the complexities of running such platforms and setting up analytical instruments are limiting. In this regard, we aimed to demonstrate a modified Transwell migration assay by introducing the retroreflection principle to the cell quantification procedures that ensure a simplified optical setup, assure easy signal acquisition, and are compatible with conventional platforms. To demonstrate retroreflection as a signaling principle, a half-metal-coated silica particle that can induce interior retroreflection was synthesized. Because the RJPs can concentrate incident light and reflect it back to the light source, retroreflection was distinctively recognizable and enabled sensitive visualization. To verify the applicability of the developed migration assay, cell quantification during the incremental progress of macrophage migration, and cell quantification under gradients of chemoattractant monocyte protein-1, was accomplished by obtaining phagocytosed RJP-mediated retroreflection signals. Considering that conventional assays are designed as endpoint measurements, we anticipate the proposed retroreflection-based cell quantification technique to be a promising solution, bypassing current limitations.

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Section: Methodsmentioning

confidence: 99%

Nonspectroscopic Migratory Cell Monitoring Method Using Retroreflective Janus Microparticles

et al. 2020

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“…The intensity of the light re-ected at the particles is signicantly brighter than the incoming light. 165 Synthesis and the phenomenon of retrore-ection are described in ref. 165. 4.8.2.…”

Section: Alternately Binding Quenching Probe Competitive Lamp (Abc-lamp)mentioning

confidence: 99%

Loop-mediated isothermal amplification (LAMP) – review and classification of methods for sequence-specific detection

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et al. 2020

Anal. Methods

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“…The authors have used monoclonal Ab clones 19C7 and 560 which target areas between amino acids 41–49 and 83–93, respectively, targeting epitopes within the stable region of cTnI. Han et al have developed novel retroreflective Janus microparticles as immunosensing probes in an optical detection-based immunoassay [38]. This work presents a nonspectroscopic optical technique that relies on basic instrumentation for cTnI measurement, hence it is an excellent candidate for POC integration.…”

Section: Complexity Of Ctni Detectionmentioning

confidence: 99%

“…Figure 3 and Figure 4: [37,38,48,49,50,57,58,61,65,66,68,69,70,71,74,84,85,86,87,88,89,93,94,95,96,97,104,105,111,112,115,116,117,120,121,122,123,125,126,127,128,130,132,133,134,136,138,144,145,148,150,151,159,161,174,175,176,184,185,186,187,188,189,190,…”

Section: Reprinting Figuresmentioning

confidence: 99%

Point-of-Care Compatibility of Ultra-Sensitive Detection Techniques for the Cardiac Biomarker Troponin I—Challenges and Potential Value

2018

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Cardiac biomarkers are frequently measured to provide guidance on the well-being of a patient in relation to cardiac health with many assays having been developed and widely utilised in clinical assessment. Effectively treating and managing cardiovascular disease (CVD) relies on swiftly responding to signs of cardiac symptoms, thus providing a basis for enhanced patient management and an overall better health outcome. Ultra-sensitive cardiac biomarker detection techniques play a pivotal role in improving the diagnostic capacity of an assay and thus enabling a better-informed decision. However, currently, the typical approach taken within healthcare depends on centralised laboratories performing analysis of cardiac biomarkers, thus restricting the roll-out of rapid diagnostics. Point-of-care testing (POCT) involves conducting the diagnostic test in the presence of the patient, with a short turnaround time, requiring small sample volumes without compromising the sensitivity of the assay. This technology is ideal for combatting CVD, thus the formulation of ultra-sensitive assays and the design of biosensors will be critically evaluated, focusing on the feasibility of these techniques for point-of-care (POC) integration. Moreover, there are several key factors, which in combination, contribute to the development of ultra-sensitive techniques, namely the incorporation of nanomaterials for sensitivity enhancement and manipulation of labelling methods. This review will explore the latest developments in cardiac biomarker detection, primarily focusing on the detection of cardiac troponin I (cTnI). Highly sensitive detection of cTnI is of paramount importance regarding the rapid rule-in/rule-out of acute myocardial infarction (AMI). Thus the challenges encountered during cTnI measurements are outlined in detail to assist in demonstrating the drawbacks of current commercial assays and the obstructions to standardisation. Furthermore, the added benefits of introducing multi-biomarker panels are reviewed, several key biomarkers are evaluated and the analytical benefits provided by multimarkers-based methods are highlighted.

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Retroreflective Janus Microparticle as a Nonspectroscopic Optical Immunosensing Probe

Cited by 45 publications

References 35 publications

Nonspectroscopic Migratory Cell Monitoring Method Using Retroreflective Janus Microparticles

Nonspectroscopic Migratory Cell Monitoring Method Using Retroreflective Janus Microparticles

Loop-mediated isothermal amplification (LAMP) – review and classification of methods for sequence-specific detection

Point-of-Care Compatibility of Ultra-Sensitive Detection Techniques for the Cardiac Biomarker Troponin I—Challenges and Potential Value

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