Loop-mediated isothermal amplification (LAMP) – review and classification of methods for sequence-specific detection

Becherer, Lisa; Borst, Nadine; Bakheit, Mohammed A.; Frischmann, Sieghard; Zengerle, Roland; Stetten, Felix von

doi:10.1039/c9ay02246e

Cited by 267 publications

(250 citation statements)

References 185 publications

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“…Compared with RT-PCR, the RT-LAMP has the advantages of simple operation, short reaction time, no need for special instruments, and high sensitivity, which is particularly suitable for rapid onsite detection. 29,30 Yan et al, developed a RT-LAMP assay for the detection of SARS-CoV-2 in clinical samples and designed ve sets of primers that target the orf1ab gene and the spike gene for optimization of this assay. The detection of 130 clinical samples by RT-LAMP showed that the sensitivity and specicity of this method were 100%, and the detection time was 26.28 AE 4.48 min, demonstrating the RT-LAMP assay is a powerful tool for the detection of SARS-CoV-2.…”

Section: Nucleic Acid-based Detection Methodsmentioning

confidence: 99%

Diagnostic technologies for COVID-19: a review

Chen

Mao

et al. 2020

RSC Adv.

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Section: Nucleic Acid-based Detection Methodsmentioning

confidence: 99%

Diagnostic technologies for COVID-19: a review

Chen

Mao

et al. 2020

RSC Adv.

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“…For the RNA amplification, reverse transcription step must be done before the LAMP process, which is known as the RT-LAMP method. Detection of the amplicon is usually performed by observing the precipitates of magnesium pyrophosphate by-product, DNA-binding dyes, gel electrophoresis, or real-time fluorescence [30,90,91].…”

Section: Isothermal Amplification-based Methodsmentioning

confidence: 99%

Laboratory detection methods for the human coronaviruses

Shabani

Dowlatshahi

Abdekhodaie

2020

Eur J Clin Microbiol Infect Dis

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Coronaviruses are a group of envelop viruses which lead to diseases in birds and mammals as well as human. Seven coronaviruses have been discovered in humans that can cause mild to lethal respiratory tract infections. HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1 are the low-risk members of this family and the reason for some common colds. Besides, SARS-CoV, MERS-CoV, and newly identified SARS-CoV-2, which is also known as 2019-nCoV, are the more dangerous viruses. Due to the rapid spread of this novel coronavirus and its related disease, COVID-19, a reliable, simple, fast, and low-cost detection method is necessary for patient diagnosis and tracking worldwide. Human coronaviruses detection methods were classified and presented in this article. The laboratory detection techniques include RT-PCR, RT-LAMP, electrochemical and optical biosensors for RNA detection, and whole virus or viral proteins detection assays.

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“…Barcoding is already used for research but implementing the same for COVID-19 diagnostic purposes requires proper validation and scaling up. For easy digitalization of results and data management, computer integration of optical sensors for barcode scanning or use of nanomaterials that change electrical or magnetic properties upon binding to target sequences can prove beneficial [91][92][93]. Another report mentions analyzing the color change of gold nanoparticles in a salt solution on amplification of the viral genomic DNA [94].…”

Section: Challenges and Prospectsmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic

Augustine

Hasan

Das

et al. 2020

Biology

185

166

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The rampant spread of COVID-19 and the worldwide prevalence of infected cases demand a rapid, simple, and cost-effective Point of Care Test (PoCT) for the accurate diagnosis of this pandemic. The most common molecular tests approved by regulatory bodies across the world for COVID-19 diagnosis are based on Polymerase Chain Reaction (PCR). While PCR-based tests are highly sensitive, specific, and remarkably reliable, they have many limitations ranging from the requirement of sophisticated laboratories, need of skilled personnel, use of complex protocol, long wait times for results, and an overall high cost per test. These limitations have inspired researchers to search for alternative diagnostic methods that are fast, economical, and executable in low-resource laboratory settings. The discovery of Loop-mediated isothermal Amplification (LAMP) has provided a reliable substitute platform for the accurate detection of low copy number nucleic acids in the diagnosis of several viral diseases, including epidemics like Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS). At present, a cocktail of LAMP assay reagents along with reverse transcriptase enzyme (Reverse Transcription LAMP, RT-LAMP) can be a robust solution for the rapid and cost-effective diagnosis for COVID-19, particularly in developing, and low-income countries. In summary, the development of RT-LAMP based diagnostic tools in a paper/strip format or the integration of this method into a microfluidic platform such as a Lab-on-a-chip may revolutionize the concept of PoCT for COVID-19 diagnosis. This review discusses the principle, technology and past research underpinning the success for using this method for diagnosing MERS and SARS, in addition to ongoing research, and the prominent prospect of RT-LAMP in the context of COVID-19 diagnosis.

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Loop-mediated isothermal amplification (LAMP) – review and classification of methods for sequence-specific detection

Abstract: This comprehensive review provides a systematic classification and a comparative evaluation of current sequence-specific detection methods for LAMP.

Cited by 267 publications

References 185 publications

Diagnostic technologies for COVID-19: a review

Diagnostic technologies for COVID-19: a review

Laboratory detection methods for the human coronaviruses

Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic

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