Fabrication of hesperidin nanoparticles loaded by poly lactic co-Glycolic acid for improved therapeutic efficiency and cytotoxicity

Abstract

Hesperidin, as a flavonone, is recognized as promising anti-inflammatory, antioxidant, and anticancer agent. Its poor bioavailability is crucial bottleneck for therapeutic efficacy. To enhance the stability and bioactive potentials, hesperidin-PLGA-Poloxamer 407 was successfully prepared to minimize or overcome problems associated with hesperidin absorption. The characteristics of nanohesperidin were testing by in vitro dissolution study, XRD, FTIR, PSA and SEM. Antioxidant effects of nanohesperidin were studied. The structure-activity relationship analysis with antioxidant pharmacophore has been performed by using density functional theory method and quantum chemical calculations. The structural properties were investigated using Becke three-parameter hybrid exchange and the Lee-Yang-Parr correction functional methods. Nanohesperidin was found to decrease the H 2 O 2 activity-induced DNA instability. Blood compatibility on human erythrocytes was confirmed by haemolytic and in vitro toxicity assessments. The in vitro anticancer activity of nanohesperidin towards MCF-7 cells using various parameters was carried out. The nanohesperidin was found to exert cell growth arrest, activated DNA fragmentation and induced apoptotic cell death through caspase-3 and p53-dependent pathways. These findings showed that nanohesperidin play an important role in its anticancer effects, suggesting might be used for clinical trials and can represent driving formulation for novel chemotherapeutic agents.

PLGA nanoparticles embedding molybdenum cluster salts: Influence of chemical composition on physico-chemical properties, encapsulation efficiencies, colloidal stabilities and in vitro release

“…The C=O stretch recorded for the polymer at 1750 cm -1 did not change in CNPs while the peak representing the C-O ester stretching slightly moved from 1088 cm -1 for the polymer to 1078 cm -1 and 1083 cm -1 for both molybdenum halide CNPs with a P/C ratio of 2.5, TBA 2 and CMB, respectively. The vibration corresponding to the sp3 C-H stretching was detected at 2914 cm -1 for the polymer and moved significantly between 2921 and 2924 cm -1 for all CNPs tested, indicating the existence of interactions between the polymer and the or hesperidin where authors concluded to the lack (Jang et al, 2019;Gaoncar et al, 2017) or to minor (Ali et al, 2019) chemical interaction between drug and polymer.…”

Hesperidin Loaded on Gold Nanoparticles as a Drug Delivery System for a Successful Biocompatible, Anti-Cancer, Anti-Inflammatory and Phagocytosis Inducer Model

“…Furthermore, AuNPs have the properties of being bio-compatible and non-toxic, making them preferable drug carriers 53 . Previous investigations revealed that Hsp exerts remarkable cytotoxicity towards selected human cancer cell lines such as those of the cervix, liver, larynx, and breast 23,54 . Moreover, it was demonstrated that Hsp exerts chemo-preventive activities along with apoptotic impacts that lead to the inhibition of colon carcinogenesis in mice 55 .…”

“…Hemolysis test. Hemolysis test was performed for the prepared Hsp, AuNPs and Hsp-AuNPs samples based on a previously described method 23 , with some modifications. Briefly, whole blood (100 µL) was mixed with PBS (700 µL) and 100 µL of Hsp, AuNPs or Hsp-AuNPs at five concentrations (20,80,160,240 and 320 µg mL −1 ) were added; phosphate buffer saline served as a negative control (0% hemolysis), whereas deionized distilled water served as a positive control (100% hemolysis).…”

Preparation of Aluminum Oxide Nanoparticles by Laser Ablation and a Study of Their Applications as Antibacterial and Wounds Healing Agent

“…The SEM image showed the interaction between alumina NPs and bacterial cells, clearly indicating the change in cell morphology and the aggregated particles on the cell wall. The aluminainteracted cells showed distorted cell morphology, indicating the distortion of bacterial cells [26]. Also, Murdock et al [27] observed that the behavior of alumina NPs was also influenced by particle size, shape and charge of surface.…”