Fabrication of glass microchannels by xurography for electrophoresis applications

Santana, Phillipe Pessoa de; Segato, Thiago Pinotti; Carrilho, Emanuel; Lima, Renato S.; Dossi, Nicolò; Kamogawa, Marcos Yassuo; Gobbi, Ângelo L.; Piazzeta, Maria H.O.; Piccin, Evandro

doi:10.1039/c3an36540a

Cited by 31 publications

(10 citation statements)

References 20 publications

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“…When compared with conventional CE systems, ME devices offer several advantages, including shorter analysis times, lower sample consumption, higher throughput capability, portability, and possibility of integrating multiple analytical steps in the same platform . Moreover, ME devices can be fabricated in many different substrate materials, including glass, quartz, and polymers , and several microfluidic shapes and designs can be achieved.…”

Section: Introductionmentioning

confidence: 99%

Separation of natural antioxidants using PDMS electrophoresis microchips coupled with amperometric detection and reverse polarity

et al. 2014

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This report describes the use of PDMS ME coupled with amperometric detection for rapid separation of ascorbic, gallic , ferulic, p‐coumaric acids using reverse polarity. ME devices were fabricated in PDMS by soft lithography and detection was accomplished using an integrated carbon fiber working electrode aligned in the end‐channel configuration. Separation and detection parameters were investigated and the best conditions were obtained using a run buffer consisting of 5 mM phosphate buffer (pH 6.9) and a detection voltage of 1.0 V versus Ag/AgCl reference electrode. All compounds were separated within 70 s using gated injection mode with baseline resolution and separation efficiencies between 1200 and 9000 plates. Calibration curves exhibited good linearity and the LODs achieved ranged from 1.7 to 9.7 μM. The precision for migration time and peak height provided maximum values of 4% for the intrachip studies. Lastly, the analytical method was successfully applied for the analysis of ascorbic and gallic acids in commercial beverage samples. The results achieved using ME coupled with amperometric detection were in good agreement with the values provided by the supplier. Based on the data reported here, the proposed method shows suitability to be applied for the routine analysis of beverage samples.

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Section: Introductionmentioning

confidence: 99%

Separation of natural antioxidants using PDMS electrophoresis microchips coupled with amperometric detection and reverse polarity

et al. 2014

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“…The development of inexpensive, sensitive, selective and user‐friendly analytical devices for decentralized control, whose assembly can be easily achieved even in developing countries where advanced technologies and infrastructures are not available, is a global challenge of the new century. Thus, micro Total Analytical Systems (µTAS), assembled by applying micromachining technologies to glass or polymeric materials, have received a growing interest in recent years in view of their low reagent consumption, easy portability and high speed of analysis 1–4. Miniaturized instrumentation suitable for disposable‐portable devices designed for decentralized control can be easily prepared by this approach, as proved by the recent proposal of several applications to clinical, environmental and food analysis 5–11.…”

Section: Introductionmentioning

confidence: 99%

Pencil‐Drawn Dual Electrode Detectors to Discriminate Between Analytes Comigrating on Paper‐Based Fluidic Devices but Undergoing Electrochemical Processes with Different Reversibility

et al. 2013

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A simple method is described to discriminate between analytes comigrating under on‐plate separation conditions, whose electrochemical behavior displays different reversible characters. It is based on the use of dual electrode detectors pencil‐drawn at the end of paper‐based fluidic channels defined by hydrophobic barriers. Simultaneous detection of comigrating species is achieved by applying to the upstream pencil‐drawn working electrode a potential for the oxidation (or reduction) of both analytes, while to the downstream pencil‐drawn working electrode a potential is imposed for the reverse process involving the product of the sole analyte undergoing a reversible enough electrochemical process. The performance of these inexpensive devices was preliminarily optimized by adopting hexacyanoferrate(II) as prototype species undergoing a reversible anodic process at carbon electrodes. They were then used as dual electrode detectors for thin‐layer chromatographic runs conducted on paper‐based microfluidic devices. Two types of synthetic solutions, one containing different contents of dopamine (DA) and ascorbic acid (AA) and the other of paracetamol (PA) and AA, were chosen as model samples. This choice was prompted us by the fact that in both cases these analytes comigrated under the adopted experimental conditions and required similar enough oxidation potentials. Nevertheless, DA and PA underwent reversible enough anodic processes while an irreversible electrochemical reaction is involved in the AA oxidation. Satisfactory results were found for both couples of target analytes, whose simultaneous detection was achieved within 230 s and was characterized by good enough repeatability and sensitivity. In particular, this approach appears to be well suited for the rapid and inexpensive assembling of electrochemical detectors for flow analysis systems.

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“…De Santana et al recently evaluated the feasibility of xurography to fabricate masks in a hydrofluoric acid (HF) etching process for the development of electrophoretic devices. Glass microchannels fabricated by xurography provided resolved, well-defined, and highly repetitive electrophoretic separation platforms [ 13 ]. Sundberg et al developed a spinning disk platform for microfluidic digital polymerase chain reactions by patterning spiral designs on 125 μm thick PETG sheets and bonding them thermally to create a laminated three-layer PCR thermocycler [ 14 ].…”

Section: Xurography and In-plane Micromixers For Poc Devicesmentioning

confidence: 99%

Xurography as a Rapid Fabrication Alternative for Point-of-Care Devices: Assessment of Passive Micromixers

Martínez-López

Mojica

Rodrı́guez

et al. 2016

Sensors

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Despite the copious amount of research on the design and operation of micromixers, there are few works regarding manufacture technology aimed at implementation beyond academic environments. This work evaluates the viability of xurography as a rapid fabrication tool for the development of ultra-low cost microfluidic technology for extreme Point-of-Care (POC) micromixing devices. By eschewing photolithographic processes and the bulkiness of pumping and enclosure systems for rapid fabrication and passively driven operation, xurography is introduced as a manufacturing alternative for asymmetric split and recombine (ASAR) micromixers. A T-micromixer design was used as a reference to assess the effects of different cutting conditions and materials on the geometric features of the resulting microdevices. Inspection by stereographic and confocal microscopy showed that it is possible to manufacture devices with less than 8% absolute dimensional error. Implementation of the manufacturing methodology in modified circular shape- based SAR microdevices (balanced and unbalanced configurations) showed that, despite the precision limitations of the xurographic process, it is possible to implement this methodology to produce functional micromixing devices. Mixing efficiency was evaluated numerically and experimentally at the outlet of the microdevices with performances up to 40%. Overall, the assessment encourages further research of xurography for the development of POC micromixers.

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Fabrication of glass microchannels by xurography for electrophoresis applications

Cited by 31 publications

References 20 publications

Separation of natural antioxidants using PDMS electrophoresis microchips coupled with amperometric detection and reverse polarity

Separation of natural antioxidants using PDMS electrophoresis microchips coupled with amperometric detection and reverse polarity

Pencil‐Drawn Dual Electrode Detectors to Discriminate Between Analytes Comigrating on Paper‐Based Fluidic Devices but Undergoing Electrochemical Processes with Different Reversibility

Xurography as a Rapid Fabrication Alternative for Point-of-Care Devices: Assessment of Passive Micromixers

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