Fabrication of double W1/O/W2 nano-emulsions loaded with oleuropein in the internal phase (W1) and evaluation of their release rate

Gharehbeglou, Pouria; Jafari, Seid Mahdi; Homayouni, Aziz; Hamishekar, Hamed; Mirzaei, Habibollah

doi:10.1016/j.foodhyd.2018.10.020

Cited by 79 publications

(21 citation statements)

References 49 publications

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“…In light of the characterization results, NLC-OLE50 seemed to be a promising carrier for OLE with enhanced antioxidant activity and thus we continued to study its efficacy in lung epithelial cell models. Several authors have reported micro-and nanoencapsulation methods for olive leaf extracts but to the best of our knowledge, there is a lack of studies regarding the impact that the formulation process could have on the bioactivity of this polyphenol in a biological system [22,23,52].…”

Section: Cell Experimentsmentioning

confidence: 99%

“…In this context, micro and nanoencapsulation technologies have been proposed as promising strategies [20]. Currently, some encapsulation methods for Olea europaea leaf extracts have been described such as W/O nanoemulsions and W/O/W double emulsions [21][22][23], spray-drying [24,25], formation of inclusion complexes with cyclodextrin [26], electrostatic extrusion [27], biopolymer complexes/double emulsions [21], and liposomes [28]. However, they have some limitations such as the use of organic solvents, tedious and time consuming manufacturing processes, high particle sizes, and low yield (for spray drying).…”

Section: Introductionmentioning

confidence: 99%

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Encapsulation of Oleuropein in Nanostructured Lipid Carriers: Biocompatibility and Antioxidant Efficacy in Lung Epithelial Cells

et al. 2020

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Oxidative damage has been linked to a number of diseases. Oleuropein (OLE), a natural occurring polyphenol from olive leaves (Olea europaea L.), is known to be a potent antioxidant compound with inherent instability and compromised bioavailability. Therefore, in this work, nanostructured lipid carriers (NLCs) were proposed for OLE encapsulation to protect and improve its antioxidant efficacy. The lipid matrix, composed of olive oil and Precirol, was optimized prior to OLE encapsulation. The characterization of the optimized oleuropein-loaded NLCs (NLC-OLE) showed a mean size of 150 nm, a zeta potential of −21 mV, an encapsulation efficiency of 99.12%, sustained release profile, and improved radical scavenging activity. The cellular in vitro assays demonstrated the biocompatibility of the NLCs, which were found to improve and maintain OLE antioxidant efficacy in the A549 and CuFi-1 lung epithelial cell lines, respectively. Overall, these findings suggest a promising potential of NLC-OLE to further design a pulmonary formulation for OLE delivery in lung epithelia.

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Section: Cell Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Encapsulation of Oleuropein in Nanostructured Lipid Carriers: Biocompatibility and Antioxidant Efficacy in Lung Epithelial Cells

et al. 2020

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“…Double (W/O/W) emulsions were used as carriers for resveratrol in order to improve its bioavailability (Wang et al, 2017 Tween 80 is related to the potential health risks and because of this, it is reasonable to use natural biomolecules such as pectin-WPC complex. The formulation of WPC-pectin double W/O/W nano-emulsions with oleuropein was also optimized by considering Span 80 (lipophilic emulsifier) content, WPC and pectin content, the ratio of inner to outer phase and pH (Gharehbeglou et al, 2019b). Optimal conditions were found to be 8.74% Span, 8% WPC, 1.97% pectin, 1:4 ratio of inner to outer phase and pH 6.1.…”

Section: Application Of Double Emulsions Loaded With Plant Bioactivesmentioning

confidence: 99%

Double emulsions (W/O/W emulsions): Encapsulation of plant bioactives

Mudrić¹,

Šavikin²,

Ibrić³

et al. 2019

Lekovite sirovine

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This article describes the preparation, characterization, and application of W/O/W emulsions, with emphasis on the encapsulation of plant bioactives. The main limitations preventing commercialization of double emulsions with plant bioactive substances, used for the preparation of food, nutraceuticalsand pharmaceuticals for oral administration, are low thermodynamic stability and the limited range of the available lipophilic emulsifiers. In that regard, strategies for stability improvement of W/O/W emulsions with bioactive substances are highlighted.

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“…[ 3 ] Surface‐active properties of various emulsifiers such as proteins (caseinate and whey proteins), polysaccharides (pectin, gum Arabic, and xanthan gum), protein–polysaccharides conjugates or complexes can be explored for the preparation of stable DE matrix. [ 7–10 ] Owing to their high molecular weight, proteins were used to form a durable and cohesive film around droplets to stabilize emulsion. [ 11 ] Polysaccharides such as pectin and gum arabic have a strong tendency to adsorb at the interface and protect droplets from aggregation by reducing Laplace pressure.…”

Section: Introductionmentioning

confidence: 99%

“…However, protein–polysaccharides conjugates/complexes are widely used for stiff interface and elastic layer around a droplet to intensify the DE stability. [ 7–10,14 ] DE stability can be enhanced by adding thickeners that increase the viscosity of the outer phase and by decreasing the mobility of oil droplets by reducing oil droplet size. [ 15 ] The stability of DE strongly depends on the electrostatic and steric stability, a viscosity of the continuous phase, and density difference between dispersed and continuous phases.…”

Section: Introductionmentioning

confidence: 99%

Emblicanin Rich Emblica officinalis Encapsulated Double Emulsion and its Antioxidant Stability during Storage

Chaudhary

Sabikhi

Hussain

et al. 2020

Euro J Lipid Sci & Tech

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Emblicanin rich water‐soluble extract of Emblica officinalis (EEO) is encapsulated in the inner phase of double emulsion (DE) by using emulsifiers in different phases at different concentrations. The effects of other variables like homogenization speed, salt and herbal concentration are also investigated on various phases of DE to obtain a stable matrix. Finally, optimized EEO encapsulated DE has 2% w/w NaCl and 50% w/w EEO in inner (W1) phase, 4% w/w polyglycerol polyricinoleate (PGPR) in middle oil‐phase and 2% w/w low‐methoxy‐pectin and reverse osmosis water in outer (W2) phase. Ultra‐Turrax high shear homogenizer is employed to prepare primary emulsion (W1/O) at 20 000 rpm and DE (W1/O in W2) at 12 000 rpm. The EEO encapsulated DE has been characterized for encapsulation efficiency (>90%), viscosity (0.715 ± 0.18 Pa s), sedimentation stability, zeta potential (−32.17 ±1.17 mV), and particle size. Light and confocal laser microscopy are used for elaborating the microscopic structure of EEO encapsulated DEs. DE has shown storage stability up to 42 days and protect antioxidant activities as compared to control (herbal extract was not encapsulated in the inner phase). The present study demonstrates that the optimized DE matrix can be used to protect the bioactive properties of EEO for its use in functional food formulation.

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Fabrication of double W1/O/W2 nano-emulsions loaded with oleuropein in the internal phase (W1) and evaluation of their release rate

Cited by 79 publications

References 49 publications

Encapsulation of Oleuropein in Nanostructured Lipid Carriers: Biocompatibility and Antioxidant Efficacy in Lung Epithelial Cells

Encapsulation of Oleuropein in Nanostructured Lipid Carriers: Biocompatibility and Antioxidant Efficacy in Lung Epithelial Cells

Double emulsions (W/O/W emulsions): Encapsulation of plant bioactives

Emblicanin Rich Emblica officinalis Encapsulated Double Emulsion and its Antioxidant Stability during Storage

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