Fabrication of DNA, o-phenylenediamine, and gold nanoparticle bioimprinted polymer electrochemical sensor for the determination of dopamine

Rezaei, Behzad; Boroujeni, Malihe Khalili; Ensafi, Ali A.

doi:10.1016/j.bios.2014.12.009

Cited by 94 publications

(37 citation statements)

References 38 publications

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“…Other reported DNA-based biosensors using binding affinities of analytes to DNA have been employed for various studies, such as in the detection of pathogenic microorganisms, other types of drugs, toxic compounds, carcinogens, and pollutants, such as Sudan II, 6 thalidomide, 18 leuprolide 19 and taxol. 20 In recent years, the inclusion of nanomaterials in biosensors to improve the sensitivities and detection limits has shown great progress and advancement.…”

Section: Introductionmentioning

confidence: 99%

“…Lately, recent developments in DNA-based biosensors have focused on the interaction of drugs and DNA, providing a convenient method for detecting drugs, possible miniaturization as well as portability. 6 Additionally, the construction of DNA sensors has gained considerable attention owing to the excellent ability of DNA to attach molecules covalently (by chemically modifying various DNA components) and/or non-covalently (by outer electrostatic interaction, groove binding, and intercalation) with high affinity and specificity. [7][8][9][10][11][12][13][14] In DNA-electrochemical biosensors, DNA is used as a biomolecular recognition element, where specific binding of molecules with DNA can be measured electrochemically and quantified.…”

Section: Introductionmentioning

confidence: 99%

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A Simple DNA-based Electrochemical Biosensor for Highly Sensitive Detection of Ciprofloxacin Using Disposable Graphene

Lim

Ahmed

2016

ANAL. SCI.

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In this work we exploited the electrostatic interaction of double stranded DNA (dsDNA) with drug components to construct a simple, but highly sensitive, DNA-electrochemical sensor for detecting ciprofloxacin. The following straightforward three-step procedure was performed to determine ciprofloxacin: (i) dsDNA-layer immobilization on the surface of the working graphene-modified screen-printed carbon electrode; (ii) dsDNA-ciprofloxacin interaction for 2 min; and (iii) electrochemical measurement using square-wave voltammetry. An increased oxidation of the guanine component was observed, at +1.0 V, as a result of the electrostatic interaction of positively charged ciprofloxacin with the negatively charged nucleic acid sugar phosphate. Based on the International Conference on Harmonization Guidelines, a linear relationship between the guanine oxidation peak and ciprofloxacin concentration (0.1 to 100 μM) was obtained with a detection limit of 0.1 μM. Our developed sensor is straightforward to construct and use, requiring no multi-step time-consuming preconditioning of electrodes. It is highly sensitive and selective in the detection of ciprofloxacin, and has the potential to be useful in the future fabrication of rapid and portable on-site food safety analysis devices.Keywords Ciprofloxacin, DNA electrochemical biosensor, graphene, screen-printed electrodes

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Simple DNA-based Electrochemical Biosensor for Highly Sensitive Detection of Ciprofloxacin Using Disposable Graphene

Lim

Ahmed

2016

ANAL. SCI.

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“…This factor is very important because major sources of interference, such as ascorbic acid (AA), also exist in body fluids together with DA and show very high concentrations with similar oxidization potentials to that of DA. Hence, a variety of composites, such as tyrosinase-based materials, (7) ion-exchange membranes, (8,9) conducting polymer films, (10) and molecularly imprinted polymer, (11) have been used to enhance the selectivity of DA detection. It is well known that the cation exchange polymer, Nafion, possesses the ability to repel negative ions and combine with positively charged species, because of the appearance of anionic sites in its structure.…”

Section: Introductionmentioning

confidence: 99%

Microelectrode Arrays Modified with Nafion/Nanoporous AuPt Nanoparticles for in vivo Detection of Dopamine

2018

Sensors and Materials

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Electrochemical determination using implantable microelectrode arrays provides a powerful tool for recording neurotransmitter concentrations across multiple spatial locations of brain tissue. In this study, nanoporous AuPt alloy nanoparticles with very rough surfaces and Nafion film were sequentially electrodeposited on Michigan-type microelectrode arrays to improve sensitivity and selectivity to dopamine (DA). The modified microelectrode arrays were characterized by scanning electron microscopy (SEM), X-ray diffractometry, cyclic voltammetry (CV), and amperometry. The results of SEM experiments indicated that the obtained AuPt alloy nanoparticles with many different sizes of pores possessed very rough surfaces and exhibited cauliflower-like shapes. During amperometric measurements, the constructed DA sensors showed a wide linear range of 0.05-12.05 μM with a high sensitivity of 73.4 ± 3.1 pA/μM. Other important features include a low detection limit of 25 nM (signalto-noise ratio: 3), excellent selectivity over ascorbic acid (AA), and good stability. The feasibility of in vivo DA detection using the modified microelectrode arrays was verified by recording electrically evoked DA in the striatum of rats. The developed DA sensors based on microelectrode arrays provide an important tool for electrochemical detection of in vivo DA.

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“…However, electrochemical determination of DA has the challenge of eliminating interfering substances (such as ascorbic acid, uric acid and so on) that exist together with DA in biological fluids. Since these interfering molecules are electroactive and their redox reactions take place at similar potentials to that of DA at unmodified electrodes, results in overlapping of voltammetric response and thus poor selectivity [21][22][23]. As a result, modification of the electrode surface with suitable modifier is the interest of research in electroanalysis to improve sensitivity and selectivity.…”

Section: Introductionmentioning

confidence: 99%

Selective electrochemical determination of dopamine at p-nitroaniline film-hole modified glassy carbon electrodes

Olana¹,

Kitte²,

Geletu³

et al. 2018

Bull. Chem. Soc. Eth.

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ABSTRACT. Herein, we report determination of dopamine (DA) at modified glassy carbon electrode (GCE) with a film produced by reduction of diazonium generated from p-nitroaniline (PNA). Pores were created purposely by stripping pre-deposited gold nanoparticles (AuNPs) in the modifier film. The modified electrodes were characterized electrochemically by common redox probes: hydroquinone (HQ), hexacyanoferrate [(Fe(CN) . Comparison was made for the cyclic voltammetric and amperometric response of DA using the modified electrodes against the bare GCE in phosphate buffer solution (PBS) of pH 7.5. The bare and modified GCE showed a linear response to DA in the concentration range of 0.2-2.2 mM and 5-35 µM with detection limit of 0.015 mM and 0.6112 µM, respectively. The modified electrode showed high sensitivity, well selectivity, good anti-interference ability, durable stability and good electrode reproducibility for determining DA. The reported modified electrode is a promising sensor for use in electroanalysis of DA.

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Fabrication of DNA, o-phenylenediamine, and gold nanoparticle bioimprinted polymer electrochemical sensor for the determination of dopamine

Cited by 94 publications

References 38 publications

A Simple DNA-based Electrochemical Biosensor for Highly Sensitive Detection of Ciprofloxacin Using Disposable Graphene

A Simple DNA-based Electrochemical Biosensor for Highly Sensitive Detection of Ciprofloxacin Using Disposable Graphene

Microelectrode Arrays Modified with Nafion/Nanoporous AuPt Nanoparticles for in vivo Detection of Dopamine

Selective electrochemical determination of dopamine at p-nitroaniline film-hole modified glassy carbon electrodes

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