Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells

Noh, Yong Kwan; Costa, Avelino Dos Santos Da; Park, Yong Seek; Du, Pu; Kim, Ik Hwan; Park, Kwideok

doi:10.1016/j.carbpol.2019.05.039

Cited by 63 publications

(34 citation statements)

References 50 publications

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“…Therefore, instead of a single-use of natural (e.g., collagen, gelatin, alginate, hyaluronic acid, and chitosan) synthetic polymers (e.g., PLGA, PLA, and polycaprolactone (PCL)) and bioceramics (the calcium phosphates (Ca/P) as hydroxyapatite (HAp), the bioactive glasses and the glass-ceramics), composite forms of them have been widely used for bone tissue engineering [ 4 , 5 ]. Compared with these natural materials, bacterial cellulose nanocrystal (BC) has much higher mechanical properties, which are required in most cases when used as a scaffold in bone tissue engineering There are remarkable features of bacterial cellulose that make it applicable in bone tissue engineering: biocompatibility, promoting cellular interactions and tissue development, having interconnected porous structure and significant effects on cell adhesion and proliferation, high purity level, microporosity, biodegradability, bio-absorbable, non-toxicity, resembling extracellular matrix of living tissue, and crystallinity [ 6 , 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

3D Printed Polycaprolactone/Gelatin/Bacterial Cellulose/Hydroxyapatite Composite Scaffold for Bone Tissue Engineering

et al. 2020

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Three-dimensional (3D) printing application is a promising method for bone tissue engineering. For enhanced bone tissue regeneration, it is essential to have printable composite materials with appealing properties such as construct porous, mechanical strength, thermal properties, controlled degradation rates, and the presence of bioactive materials. In this study, polycaprolactone (PCL), gelatin (GEL), bacterial cellulose (BC), and different hydroxyapatite (HA) concentrations were used to fabricate a novel PCL/GEL/BC/HA composite scaffold using 3D printing method for bone tissue engineering applications. Pore structure, mechanical, thermal, and chemical analyses were evaluated. 3D scaffolds with an ideal pore size (~300 µm) for use in bone tissue engineering were generated. The addition of both bacterial cellulose (BC) and hydroxyapatite (HA) into PCL/GEL scaffold increased cell proliferation and attachment. PCL/GEL/BC/HA composite scaffolds provide a potential for bone tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

3D Printed Polycaprolactone/Gelatin/Bacterial Cellulose/Hydroxyapatite Composite Scaffold for Bone Tissue Engineering

et al. 2020

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“…Furthermore, chitosan was found to play a significant roles in inflammatory cells, and promote granulation [22]. Collagen is known as one of the main proteins in the skin, which is biocompatible and can support cell and tissue growth [23]. Therefore, collagen has become a promoting candidate for wound dressing material, since active wound dressings must be chemically similar to a protein structure that can support the proliferation, and enhance fibroblast cell growth, in human skin [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

“…Previous research is limited in fabricating BC mats with chitosan or collagen. Noh et al, reported the use of BC/collagen for wound dressing or scaffolding on human mesenchymal stem cells [23]. Another study by Jia et al, reported the use of BC/chitosan bio-hydrogel for potential scaffolding in tissue engineering [27].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Bacterial Cellulose-Based Wound Dressing in Different Order Impregnation of Chitosan and Collagen

et al. 2020

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Bacterial cellulose (BC), chitosan (Chi), and collagen (Col) are known as biopolymers which have met some properties that are required as wound dressing. This study focused on investigating the fabrication of BC-based wound dressing with chitosan and collagen, since chitosan has red blood cells binding and anti-bacterial properties, while collagen can support cell and tissue growth for skin wounds. The BC-based wound dressing was prepared by impregnating BC fibers in the chitosan and/or collagen solution for 24 h. FTIR was used to confirm the intermolecular interaction of amine and hydroxyl group of chitosan and/or collagen in BC-based wound dressing. Furthermore, the XRD diffractogram of the wound dressing show broader peaks at 14.2°, 16.6°, and 22.4° due to the presence of chitosan and collagen molecules in BC fibers. These results were then supported by SEM images which confirmed that chitosan and collagen were well penetrated into BC fibers. TGA curves revealed that BC/Chi/Col has better thermal properties based on the Tmax compare to BC/Col/Chi. Feasibility of the mats to be applied as wound dressing was also supported by other tests, i.e., water content, porosity, and hemocompatibility, which indicates that the wound dressing is classified as nonhemolytic materials. However, BC/Col/Chi was considered a more potential wound dressing to be applied compared to BC/Chi/Col since it has larger pores and showed better antibacterial properties (larger zones of inhibition) against S. aureus and E. coli via disk diffusion tests.

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“…The obtained results indicated that FSCP/COS nanofibers are good support for fibroblast proliferation. In another study, Noh et al [116] introduced bacterial cellulose-collagen composite scaffolds in different ratios (1:1, 3:1, 5:1) to assess their impact on human mesenchymal stem cells (hMSCs). In comparison to pure collagen scaffolds, the composite presented better physical stability and higher water uptake by increasing the bacterial cellulose content.…”

Section: Natural Polymer-natural Polymer Composite Nanofibersmentioning

confidence: 99%

Electrospun Nanofibers of Natural and Synthetic Polymers as Artificial Extracellular Matrix for Tissue Engineering

et al. 2020

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Many types of polymer nanofibers have been introduced as artificial extracellular matrices. Their controllable properties, such as wettability, surface charge, transparency, elasticity, porosity and surface to volume proportion, have attracted much attention. Moreover, functionalizing polymers with other bioactive components could enable the engineering of microenvironments to host cells for regenerative medical applications. In the current brief review, we focus on the most recently cited electrospun nanofibrous polymeric scaffolds and divide them into five main categories: natural polymer-natural polymer composite, natural polymer-synthetic polymer composite, synthetic polymer-synthetic polymer composite, crosslinked polymers and reinforced polymers with inorganic materials. Then, we focus on their physiochemical, biological and mechanical features and discussed the capability and efficiency of the nanofibrous scaffolds to function as the extracellular matrix to support cellular function.

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Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells

Cited by 63 publications

References 50 publications

3D Printed Polycaprolactone/Gelatin/Bacterial Cellulose/Hydroxyapatite Composite Scaffold for Bone Tissue Engineering

3D Printed Polycaprolactone/Gelatin/Bacterial Cellulose/Hydroxyapatite Composite Scaffold for Bone Tissue Engineering

Characterization of Bacterial Cellulose-Based Wound Dressing in Different Order Impregnation of Chitosan and Collagen

Electrospun Nanofibers of Natural and Synthetic Polymers as Artificial Extracellular Matrix for Tissue Engineering

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