Fabrication of a biomimetic ZeinPDA nanofibrous scaffold impregnated with BMP‐2 peptide conjugated TiO ₂ nanoparticle for bone tissue engineering

Abstract

A biomimetic Zein polydopamine based nanofiber scaffold was fabricated to deliver bone morphogenic protein-2 (BMP-2) peptide conjugated titanium dioxide nanoparticles in a sustained manner for investigating its osteogenic differentiation potential. To prolong its retention time at the target site, BMP-2 peptide has been conjugated to titanium dioxide nanoparticles owing to its high surface to volume ratio. The effect of biochemical cues from BMP-2 peptide and nanotopographical stimulation of electrospun Zein polydopamine nanofiber were examined for its enhanced osteogenic expression of human fetal osteoblast cells. The sustained delivery of bioactive signals, improved cell adhesion, mineralization, and differentiation could be attributed to its highly interconnected nanofibrous matrix with unique material composition. Further, the expression of osteogenic markers revealed that the fabricated nanofibrous scaffold possess better cell-biomaterial interactions. These promising results demonstrate the potential of the composite nanofibrous scaffold as an effective biomaterial substrate for bone regeneration.

Peptide Self-Assembly into Hydrogels for Biomedical Applications Related to Hydroxyapatite

“…Thus, the bone morphogenetic protein-2 biomimetic peptide (BMPBP) has been proposed considering its reduced size (i.e., between 20 and 30 amino acids) and the presence of typical SSPVT morphogenic sequences. Scaffolds encapsulating BMPBP were able to induce osteogenic differentiation and promote bone tissue regeneration [163,164]. However, these scaffolds could not provide sufficient bioactivity to meet the requirements of clinical application.…”

Modulation of Amyloidogenesis Controlled by the C-Terminal Domain of Islet Amyloid Polypeptide Shows New Functions on Hepatocyte Cholesterol Metabolism

“…A nature-based approach, i.e., biomimetics associated with the adaptation of models inspired by biology, has been the basis for the generation of new systems or materials ( 15 ). The tendency to form IAPP-amyloid structures of hundreds of organisms has not been completely analyzed.…”

Bone regeneration by freeze‐dried composite of octacalcium phosphate collagen and teriparatide

“…In addition, that of OCPcolTPTDf 0.1 or β‐TCPcolTPTDf 0.1 demonstrated about one‐quarter of the TPTD was released within 7 days, and three‐quarters remained at 28 days. These results resembled the release profile of bone morphogenic protein‐2 (BMP‐2) from Zein polydopamine TiO2 BMP‐2, which could have triggered early differentiation markers and activated the expression of late osteogenic markers (Babitha et al, ). The release pattern of OCPcolTPTDf or β‐TCPcolTPTDf suggested that TPTD was retained in OCPcol or β‐TCPcol after initial release.…”