Fabrication, characterization and evaluation of the effect of PLGA and PLGA–PEG biomaterials on the proliferation and neurogenesis potential of human neural SH‐SY5Y cells

Abstract

In recent years with regard to the development of nanotechnology and neural stem cell discovery, the combinatorial therapeutic strategies of neural progenitor cells and appropriate biomaterials have raised the hope for brain regeneration following neurological disorders. This study aimed to explore the proliferation and neurogenic effect of PLGA and PLGA-PEG nanofibers on human SH-SY5Y cells in in vitro condition.Nanofibers of PLGA and PLGA-PEG biomaterials were synthesized and fabricated using electrospinning method. Physicochemical features were examined using HNMR, FT-IR, and water contact angle assays. Ultrastructural morphology, the orientation of nanofibers, cell distribution and attachment were visualized by SEM imaging. Cell survival and proliferation rate were measured. Differentiation capacity was monitored by immunofluorescence staining of Map-2. HNMR, FT-IR assays confirmed the integration of PEG to PLGA backbone. Water contact angel assay showed increasing surface hydrophilicity in PLGA-PEG biomaterial compared to the PLGA substrate. SEM analysis revealed the reduction of PLGA-PEG nanofibers' diameter compared to the PLGA group. Cell attachment was observed in both groups while PLGA-PEG had a superior effect in the promotion of survival rate compared to other groups (p < .05). Compared to the PLGA group, PLGA-PEG increased the number of Ki67 + cells (p < .01). PLGA-PEG biomaterial induced neural maturation by increasing protein Map-2 compared to the PLGA scaffold in a three-dimensional culture system.According to our data, structural modification of PLGA with PEG could enhance orientated differentiation and the dynamic growth of neural cells.

Construction of recombinant Omp25 or EipB protein loaded PLGA nanovaccines for Brucellosis protection

“…The significant decrease in the intensity of the main peaks at 1650 and 1540 cm −1 attributed to rOmp25 and rEipB in the spectra of O-NPs and E-NPs, with dominant characteristic peaks of PLGA, indicates the encapsulation of recombinant proteins into PLGA nanoparticles. However, the presence of amid-I and amid-II bonds at 1650 and 1540 cm −1 , respectively, albeit with low intensity in the FT-IR spectrum of nanoparticles, suggests some protein adsorption onto the surface of the nanoparticles [35,47].…”

“…The band at 1750 cm −1 represents the stretching vibration of the ester bond C=O, while bands at 1165 and 1087 cm −1 correspond to the stretching vibrations of C-O [35,47]. The significant decrease in the intensity of the main peaks at 1650 and 1540 cm −1 attributed to rOmp25 and rEipB in the spectra of O-NPs and E-NPs, with dominant characteristic peaks of PLGA, indicates the encapsulation of recombinant proteins into PLGA nanoparticles.…”