Fabrication and evaluation of biomimetic scaffolds by using collagen–alginate fibrillar gels for potential tissue engineering applications

Biological evaluation of human hair keratin scaffolds for skin wound repair and regeneration

“…1), in agreement with the reported data [21,31]. Freeze-drying of reductive keratin solutions, according to our previously developed protocol [4], yielded keratin scaffolds with open pores and good porous architecture (Fig. 2).…”

“…A previously developed freeze-drying protocol was applied to fabricate keratin scaffolds [4]. For this purpose, three groups of reductive solutions at concentrations of 2 wt.%, 4 wt.% and 8 wt.% keratins were prepared by diluting the dialyzed solution which contained 9.2 wt.% keratins.…”

“…Their porous architecture secures the diffusion of nutrients and metabolites for the cell population and permits in-growth and differentiation of cells [3,4]. Seeding and culturing of selected target cells onto these scaffolds gives rise to different tissue-engineered constructs ex vivo, and meanwhile scaffolds could be also directly used in vivo as tissue substitutes which play critical roles in repairing various tissue defects [5].…”

Collagen-Based Drug Delivery Systems for Tissue Engineering

“…Hyaluronic acid (Davidenko et al, 2010) Matrix, membrane, hydrogel, fibers Silk fibroin (Zhou et al, 2010) Membrane, fibers, matrix, microtubes Chondroitin-6-suphate (Stadlinger et al, 2008) Tube, matrix Elastin Tube, film, fibers, matrix Alginate (Sang et al, 2011) Spongious, filler for bone, Chitosan (Sionkowska et al, 2004) Matrix, membrane, tubular graft, nanofibers, hydrogel, Heparin (Stamov et al, 2008) Matrix Collagensynthetic polymer Poly-L-lactide (PLLA) (Chen et al, 2006) Coating for composite Poly-lactic-co-glycolic-acid (PLGA) (Wen et al, 2007) Fibers, matrix, coated tube ε-caprolactone (Schnell et al, 2007) Nanofibers Poly(ethylene-glycol) (PEG) Films, fibers…”

Bioactive surfaces from seaweed-derived alginates for the cultivation of human stem cells

“…In addition, a transfer to spherical hydrogels is difficult regarding a proper presentation of the proteins at the surface (Terazono et al 2012). Non-covalent mixing of these components for bioactivation represents a more suitable strategy for complete cell encapsulation in hydrogels (Sang et al 2011). Furthermore, defined stem cell culture media do not provide any proteins that can adsorb on the alginate's surface and trigger cell adhesion (Fadeev and Melkoumian 2011).…”