Biological evaluation of human hair keratin scaffolds for skin wound repair and regeneration

Xu, Songmei; Sang, Lin; Zhang, Jian Ping; Wang, Xiaoliang; Li, Xudong

doi:10.1016/j.msec.2012.10.011

Cited by 117 publications

(69 citation statements)

References 44 publications

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“…Keratin, particularly “hard” keratin, has been used in scaffolds and drug delivery carriers for skin [22], muscle [23], and nerve tissue engineering [23–29]. Furthermore, extracellular “hard” keratin has been successfully used in the treatment of dermal burn wounds on animal models (split-thickness burns in mice, rats [30], or pigs [31]) and on clinical patients (split-thickness burns <10% of total body surface area [22]). We previously reported a method for three-dimensional (3D) printing hair-derived keratin hydrogels using a riboflavin-sodium persulfate-hydroquinone (initiator-catalyst-inhibitor) photosensitive resin [32].…”

Section: Introductionmentioning

confidence: 99%

Development of keratin-based membranes for potential use in skin repair

et al. 2019

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The layers in skin determine its protective and hemostasis functions. This layered microstructure cannot be naturally regenerated after severe burns; we aim to reconstruct it using guided tissue regeneration (GTR). In GTR, a membrane is used to regulate tissue growth by stopping fast-proliferating cells and allowing slower cells to migrate and reconstruct specialized microstructures. Here, we proposed the use of keratin membranes crosslinked via dityrosine bonding. Variables from the crosslinking process were grouped within an energy density (ED) parameter to manufacture and evaluate the membranes. Sol fraction, spectrographs, and thermograms were used to quantify the non-linear relation between ED and the resulting crosslinking degree (CD). Mechanical and swelling properties increased until an ED threshold was reached; at higher ED, the CD and properties of the membranes remained invariable indicating that all possible dityrosine bonds were formed. Transport assays showed that the membranes allow molecular diffusion; low ED membranes retain solutes within their structure while the high ED samples allow higher transport rates indicating that uncrosslinked proteins can be responsible of reducing transport. This was confirmed with lower transport of adipogenic growth factors to stem cells when using low ED membranes; high ED samples resulted in increased production of intracellular lipids. Overall, we can engineer keratin membranes with specific CD, a valuable tool to tune microstructural and transport properties.

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Section: Introductionmentioning

confidence: 99%

Development of keratin-based membranes for potential use in skin repair

et al. 2019

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Section: Introductionmentioning

confidence: 99%

“…[19][20][21][22][23][24][25][26][27][28] KER possesses amino acid sequences similar to those found on an extracellular matrix (ECM), and because ECM is known to interact with integrins, which enable it to support cellular attachment, proliferation, and migration, KER-based materials are expected to have such properties as well. [19][20][21][22][23][24][25][26][27][28] Furthermore, KER is known to possess advantages for wound care, tissue reconstruction, cell seeding and diffusion, and drug delivery. [11][12][13][14][15][16][17][18][19][20] Unfortunately, in spite of its unique properties, KER has relatively poor mechanical properties, and as a consequence, it was not possible to fully exploit the unique properties of KER for various applications.…”

Section: Introductionmentioning

confidence: 99%

“…[11][12][13][14][15][16][17][18][19][20] Unfortunately, in spite of its unique properties, KER has relatively poor mechanical properties, and as a consequence, it was not possible to fully exploit the unique properties of KER for various applications. [19][20][21][22][23][24][25][26][27][28] To increase the structural strength of KER-based materials, attempts have been made to cross-link KER chains with a cross-linking agent or introduce functional groups to its amino acid residues via chemical reaction(s). [19][20][21][22][23][24][25][26][27][28] The rather complicated, costly, and multistep process is not desirable because it may inadvertently alter its unique properties, making the KER-based materials less biocompatible and toxic and removing or lessening its unique properties.…”

Section: Introductionmentioning

confidence: 99%

“…[19][20][21][22][23][24][25][26][27][28] To increase the structural strength of KER-based materials, attempts have been made to cross-link KER chains with a cross-linking agent or introduce functional groups to its amino acid residues via chemical reaction(s). [19][20][21][22][23][24][25][26][27][28] The rather complicated, costly, and multistep process is not desirable because it may inadvertently alter its unique properties, making the KER-based materials less biocompatible and toxic and removing or lessening its unique properties. A new method that can improve the structural strength of KER-based products not by synthetic methods but rather by the use of naturally occurring polysaccharides such as CEL is particularly needed.…”

Section: Introductionmentioning

confidence: 99%

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One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity

Tran

Prosenc

Franko

et al. 2016

ACS Appl. Mater. Interfaces

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A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag + ) or Ag 0 nanoparticles (Ag + NPs or Ag 0 NPs). In this method, butylmethylimmidazolium chloride ([BMIm + Cl -]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm + Cl -] solution of [CEL+KER] during the dissolution process. The silver in the composites can be maintained as ionic silver (Ag + ) or completely converted to metallic silver (Ag 0 ) by reducing it with NaBH4. The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites. Powder XRD and SEM results show that the silver in the [CEL+KER+Ag + ] and [CEL+KER+Ag 0 ] composites is homogeneously distributed throughout the composites in either Ag + (in the form of AgClNPs) or Ag 0 NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively. Both composites were found to exhibit excellent antibacterial activity against many bacteria including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, methicillin-resistant S. aureus (MRSA), and vancomycin-resistant Enterococus faecalis (VRE). The antibacterial activity of both composites increases with the Ag + or Ag 0 content in the composites. More importantly, for the same bacteria and the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than [CEL+KER+Ag 0 NPs] composite. Experimental results confirm that there was hardly any Ag 0 NPs release from the [CEL+KER+Ag 0 NPs] composite, and hence its antimicrobial activity and NOT THE PUBLISHED VERSION; this is the author's final, peer-reviewed manuscript. The published version may be accessed by following the link in the citation at the bottom of the page. Vol 8, No. 50 (2016): pg. 34791-34801. DOI. This article is © American Chemical Society and permission has been granted for this version to appear in e-Publications@Marquette. American Chemical Society does not grant permission for this article to be further copied/distributed or hosted elsewhere without the express permission from American Chemical Society. ACS Applied materials & Interfaces,3 biocompatibility is due not to any released Ag 0 NPs but rather entirely to the Ag 0 NPs embedded in the composite. Both AgClNPs and Ag 0 NPs were found to be toxic to human fibroblasts at higher concentration (>0.72 mmol), and for the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than the [CEL+KER+Ag 0 NPs] composite. As expected, by lowering the Ag 0 NPs concentration to 0.48 mmol or less, the [CEL+KER+Ag 0 NPs] composite can be made biocompatible while still retaining its antimicrobial activity against bacteria such as E. coli, S. aureus, P. aeruginosa, MRSA, and VRE. These results, together with our previous finding that [CEL+KER] composites can ...

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Insight into the Regulatory Function of Human Hair Keratins in Wound Healing Using Proteomics

Gao

Kan

et al. 2020

Adv. Biosys.

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Keratins derived from human hair possess excellent wound healing qualities. However, their functional contribution to this process is poorly understood. In this study, the regulatory function of human hair keratins in wound healing is investigated using proteomic analysis by dividing keratins into different groups based on their molecular weight distributions: low molecular weight keratins (LMWK, 10–30 kDa), medium molecular weight keratins (MMWK, 30–50 kDa), and high molecular weight keratins (HMWK, >50 kDa). Keratin hydrogels with different molecular weights exhibit various morphologies, rheological properties, degradation rates, and wound healing activities. Using proteomic analysis, LMWK and HMWK hydrogels exhibit a stronger regulatory ability for wound healing at days 1 and 7, respectively. The major functions of LMWK during wound healing are regulation of cells communication and function. In contrast, proteins associated with energy metabolism are significantly expressed after HMWK hydrogel treatment at day 1, and these play an important role in cellular growth and reactive oxygen species scavenging at day 7. These results demonstrate that the wound healing qualities of human hair keratins are influenced by their molecular weight distribution, and the proteomic analysis sheds new light on the regulatory function of human hair keratins during wound healing.

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Biological evaluation of human hair keratin scaffolds for skin wound repair and regeneration

Cited by 117 publications

References 44 publications

Development of keratin-based membranes for potential use in skin repair

Development of keratin-based membranes for potential use in skin repair

One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity

Insight into the Regulatory Function of Human Hair Keratins in Wound Healing Using Proteomics

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