Fabrication and biocompatibility of cell scaffolds of poly(L-lactic acid) and poly(L-lactic-co-glycolic acid)

Shi, Gaoquan; Cai, Qing; Wang, Changyong; Lu, Ning; Wang, Shenguo; Bei, Jianzhong

doi:10.1002/pat.178.abs

Cited by 24 publications

(28 citation statements)

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“…Biocompatibility is an important issue for applying biomaterials in vivo . Although the distinguishing biocompatibility of PLGA has been well accepted [27] , it is still necessary to find out the toxic effect of the PLGA microspheres prepared. The MTT assay results suggested that the microspheres are biocompatible and can be used for controlled drug release.…”

Section: Resultsmentioning

confidence: 99%

Controlled Release of Dutasteride from Biodegradable Microspheres: In Vitro and In Vivo Studies

et al. 2014

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The aim of the present work was to study the in vitro/in vivo characteristics of dutasteride loaded biodegradable microspheres designed for sustained release of dutasteride over four weeks. An O/W emulsion-solvent evaporation method was used to incorporate dutasteride, which is of interest in the treatment of benign prostatic hyperplasia (BPH), into poly(lactide-co-glycolide) (PLGA). A response surface method (RSM) with central composite design (CCD) was employed to optimize the formulation variables. A prolonged in vitro drug release profile was observed, with a complete release of the entrapped drug within 28 days. The pharmacokinetics study showed sustained plasma drug concentration-time profile of dutasteride loaded microspheres after subcutaneous injection into rats. The in vitro drug release in rats correlated well with the in vivo pharmacokinetics profile. The pharmacodynamics evaluated by determination of the BPH inhibition in the rat models also showed a prolonged pharmacological response. These results suggest the potential use of dutasteride loaded biodegradable microspheres for the management of BPH over long periods.

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Section: Resultsmentioning

confidence: 99%

Controlled Release of Dutasteride from Biodegradable Microspheres: In Vitro and In Vivo Studies

et al. 2014

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“…The PLLA and PLGA were purified by dissolution of the polymer in chloroform and precipitation in ethanol, followed by drying in vacuum at room temperature for 48 h. PLGA (85/15) porous scaffolds were prepared by a solvent casting/particulate leaching technique using NaCl as the particulate porogen. 30,31 The technique used for characterization of the scaffold (pore size:194 Ϯ 44 m; porosity: 91.4%; thickness: 1.48 mm) can be seen in detail in our previous work. 32…”

Section: Methodsmentioning

confidence: 99%

Plasma‐treated, collagen‐anchored polylactone: Its cell affinity evaluation under shear or shear‐free conditions

Yang

Wan

Yang

et al. 2003

J Biomedical Materials Res

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Poly(L-lactic acid)(PLLA) and poly(L-lactic-co-glycolic acid) (PLGA) (85/15) were modified by plasma treatment. Then they were collagen anchored (PT/CA), and the cell affinity was evaluated by cell culture under shear or shear-free conditions. A convenient and "intuitionistic" dyeing method has been proposed for measuring the modified depth when plasma treatment is applied for the treatment of porous scaffolds. A parallel plate flow chamber was developed in order to study the cell affinity of a material under shear stress. Our results show that a porous scaffold can be modified by plasma treatment and that a depth of about 4.0 mm for this modification can be reached with NH(3) plasma treatment (50 w, 20 Pa, 5 min). PT/CA modification is an effective surface modification method for facilitating cell transplantation and improving the cell affinity of three-dimensional porous cell scaffolds in tissue engineering. It can solve the problem of non-uniform cell distribution in most synthetic porous cell scaffolds. Using the flow chamber system, a series of quantitative data, including cell adherent fraction, cell area, and mean shape, were compared to evaluate the cell affinity of PLLA before and after PT/CA modification. The results indicate that the quality of cell attachment on PT/CA-modified PLLA apparently is better than that on unmodified PLLA. The flow chamber system potentially may be a tool for evaluating surface modification methods.

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“…The distribution of the uniformity and interconnection of the pores is fundamental in the fabrication of scaffolds as these features facilitate the penetration of fluids via the material and promote the formation of tissue in an organized network 38. Cells that adhere to the surface of the scaffold absorb nutrients and remove metabolite through these pores 39.…”

Section: Discussionmentioning

confidence: 99%

Tailoring the surface architecture of poly(3‐hydroxybutyrate‐co‐4‐hydroxybutyrate) scaffolds

Vigneswari

Majid

Amirul

2011

J of Applied Polymer Sci

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This study was designed to determine whether the surface modifications of the various poly (3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] copolymer scaffolds fabricated would enhance mouse fibroblast cells (L929) attachment and proliferation. The P(3HB-co-4HB) copolymer with a wide range of 4HB monomer composition (16-91 mol %) was synthesized by a local isolate Cupriavidus sp. USMAA1020 by employing the modified two-stage cultivation and by varying the concentrations of 4HB precursors, namely c-butyrolactone and 1,4-butanediol. Five different processing techniques were used in fabricating the P(3HB-co-4HB) copolymer scaffolds such as solvent casting, salt-leaching, enzyme degradation, combining salt-leaching with enzyme degradation, and electrospinning. The increase in 4HB composition lowered melting temperatures (T m ) but increased elongation to break. P(3HB-co-91 mol % 4HB) exhibited a melting point of 46C and elongation to break of 380%. The atomic force analysis showed an increase in the average surface roughness as the 4HB monomer composition increased. The mouse fibroblasts (L929) cell attachment was found to increase with high 4HB monomer composition in copolymer scaffolds. These results illustrate the importance of a detailed characterization of surface architecture of scaffolds to provoke specific cellular responses.

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Fabrication and biocompatibility of cell scaffolds of poly(L-lactic acid) and poly(L-lactic-co-glycolic acid)

Cited by 24 publications

References 0 publications

Controlled Release of Dutasteride from Biodegradable Microspheres: In Vitro and In Vivo Studies

Controlled Release of Dutasteride from Biodegradable Microspheres: In Vitro and In Vivo Studies

Plasma‐treated, collagen‐anchored polylactone: Its cell affinity evaluation under shear or shear‐free conditions

Tailoring the surface architecture of poly(3‐hydroxybutyrate‐co‐4‐hydroxybutyrate) scaffolds

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